| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 308,299 | T→C | 54.4% | Q30R (CAG→CGG) | maa ← | maltose O‑acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 308,299 | 0 | T | C | 54.4% | 15.9 / 63.8 | 81 | Q30R (CAG→CGG) | maa | maltose O‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (14/23); new base C (20/24); total (34/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATCAGCGAGAATTTGCTGGCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACT > minE/308231‑308353 | gATCAGCGAGAATTTGCTGGCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCt < 1:2639874/69‑1 (MQ=255) tGGCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCg < 1:2144446/68‑1 (MQ=255) tGGCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCg < 1:599551/68‑1 (MQ=255) tGGCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCg < 1:3325195/68‑1 (MQ=255) tGGCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCg < 1:3424896/68‑1 (MQ=255) tGGCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCg < 1:1508055/68‑1 (MQ=255) tGGCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCg < 1:1780479/68‑1 (MQ=255) tGGCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCg < 1:1721521/68‑1 (MQ=255) ggCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCg < 1:2097548/67‑1 (MQ=255) tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:2575175/69‑1 (MQ=255) tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:57660/69‑1 (MQ=255) tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:2174399/69‑1 (MQ=255) tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:2394689/69‑1 (MQ=255) tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:2460302/69‑1 (MQ=255) tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:2507077/69‑1 (MQ=255) tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:2366062/69‑1 (MQ=255) tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:2674367/69‑1 (MQ=255) tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:273641/69‑1 (MQ=255) tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:285632/69‑1 (MQ=255) tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:3043745/69‑1 (MQ=255) tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:3164191/69‑1 (MQ=255) tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:3211920/69‑1 (MQ=255) tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:1059504/69‑1 (MQ=255) tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:1717566/69‑1 (MQ=255) tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:1693061/69‑1 (MQ=255) tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:928397/69‑1 (MQ=255) tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:1956833/69‑1 (MQ=255) tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:1376983/69‑1 (MQ=255) tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:1269587/69‑1 (MQ=255) tgCTCTTCCGACAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:922492/69‑1 (MQ=255) ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:3023059/1‑49 (MQ=255) ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:2962328/1‑49 (MQ=255) ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:3371364/1‑49 (MQ=255) ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:2809542/1‑49 (MQ=255) ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:1660151/1‑49 (MQ=255) ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:2068655/1‑49 (MQ=255) ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:2577174/1‑49 (MQ=255) ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:841058/1‑49 (MQ=255) ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:2605817/1‑49 (MQ=255) ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:2497661/1‑49 (MQ=255) ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:2067107/1‑49 (MQ=255) ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:2015013/1‑49 (MQ=255) ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:746876/1‑49 (MQ=255) ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:2225595/1‑49 (MQ=255) ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:555522/1‑49 (MQ=255) ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:2280686/1‑49 (MQ=255) ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:53849/1‑49 (MQ=255) ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:1863807/1‑49 (MQ=255) ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:504692/1‑49 (MQ=255) ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:393432/1‑49 (MQ=255) ctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:3031295/65‑1 (MQ=255) ccGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGAt < 1:2615585/59‑1 (MQ=255) ccGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGAt < 1:3279468/59‑1 (MQ=255) ccGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGAt < 1:72236/59‑1 (MQ=255) ccGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGAt < 1:3215312/59‑1 (MQ=255) gCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCa < 1:1454033/66‑1 (MQ=255) gCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCa < 1:1814558/66‑1 (MQ=255) ccAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTg < 1:1705059/69‑1 (MQ=255) ccAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTg < 1:2467180/69‑1 (MQ=255) ccAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTg < 1:3102738/69‑1 (MQ=255) gAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGa > 1:2432989/1‑68 (MQ=255) aTGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGc > 1:2693057/1‑68 (MQ=255) aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCTAGATAACGTCTCATCTGCCGAGCGAt > 1:3129215/1‑68 (MQ=255) aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGa > 1:1251056/1‑67 (MQ=255) aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGa > 1:2172645/1‑67 (MQ=255) aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGAt > 1:3073529/1‑68 (MQ=255) aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGAt > 1:2503483/1‑68 (MQ=255) aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGAt > 1:145431/1‑68 (MQ=255) aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGAt > 1:2489707/1‑68 (MQ=255) aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGAt > 1:2295033/1‑68 (MQ=255) aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGAt > 1:3171708/1‑68 (MQ=255) aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGAt > 1:2153388/1‑68 (MQ=255) aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGAt > 1:2041823/1‑68 (MQ=255) aTTGTATCGGTGAATAAGCTGACCAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGAt > 1:2943871/1‑68 (MQ=255) aTCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCa < 1:3001582/50‑1 (MQ=255) tCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACt < 1:2277418/68‑1 (MQ=255) tCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACt < 1:1421016/68‑1 (MQ=255) tCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACt < 1:1713765/68‑1 (MQ=255) tCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACt < 1:442256/68‑1 (MQ=255) ggTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:2947601/41‑1 (MQ=255) tGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGAt < 1:1992857/36‑1 (MQ=255) tGAATAAGCCGACGAGCTCGCTGGCGATCGCGAGATAAc < 1:951636/39‑1 (MQ=37) | GATCAGCGAGAATTTGCTGGCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACT > minE/308231‑308353 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |