BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	309,067	T→A	40.5%	V37V (GTA→GTT)	ybaJ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	309,067	0	T	A	40.5%	61.0 / 102.4	106	V37V (GTA→GTT)	ybaJ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (43/20); new base A (22/21); total (65/41)
Fisher's exact test for biased strand distribution p-value = 1.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

CGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAAC  >  minE/309010‑309133

cGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATg                                                         <  1:2487173/69‑1 (MQ=255)

cGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATg                                                         <  1:2431345/69‑1 (MQ=255)

cGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATg                                                         <  1:786310/69‑1 (MQ=255)

cGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGGCGTTTACCCAGCCATg                                                         <  1:2441744/69‑1 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTa                                                                   >  1:1926650/1‑52 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCtt                                                  >  1:1877714/1‑69 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCtt                                                  >  1:2769266/1‑69 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCtt                                                  >  1:2593404/1‑69 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCtt                                                  >  1:2388379/1‑69 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCtt                                                  >  1:2369068/1‑69 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCtt                                                  >  1:2349654/1‑69 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCtt                                                  >  1:1000449/1‑69 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCtt                                                  >  1:905138/1‑69 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAAccc                                                                >  1:624067/1‑55 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCAt                                                          >  1:303110/1‑61 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCAt                                                          >  1:3266917/1‑61 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCAt                                                          >  1:3360585/1‑61 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCAt                                                          >  1:207719/1‑61 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCAt                                                          >  1:1728578/1‑61 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCAt                                                          >  1:1607449/1‑61 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCAt                                                          >  1:2329891/1‑61 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCAt                                                          >  1:3099993/1‑61 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCAt                                                          >  1:2430346/1‑61 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCAt                                                          >  1:2539540/1‑61 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCAt                                                          >  1:2941244/1‑61 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCAt                                                          >  1:1082986/1‑61 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCAt                                                          >  1:1491276/1‑61 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCAt                                                          >  1:1444708/1‑61 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCAt                                                          >  1:2738390/1‑61 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCAt                                                          >  1:662555/1‑61 (MQ=255)

       tGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGGCGTTTACCCAGCCATGATTGCtt                                                  >  1:2369988/1‑69 (MQ=255)

       tGCTCAATCAGTTCATTAAACTGGAGGTTGATCGCCGAGGTTGGGTCGttt                                                                    >  1:418770/1‑51 (MQ=255)

         cTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAccc                                                                <  1:1386061/53‑1 (MQ=255)

         cTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAccc                                                                <  1:2930028/53‑1 (MQ=255)

         cTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAccc                                                                <  1:1334782/53‑1 (MQ=255)

         cTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAccc                                                                <  1:1539035/53‑1 (MQ=255)

         cTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAccc                                                                <  1:1588289/53‑1 (MQ=255)

                aGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAgg                                         >  1:1423775/1‑69 (MQ=255)

                aGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAgg                                         >  1:523560/1‑69 (MQ=255)

                aGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAgg                                         >  1:2483771/1‑69 (MQ=255)

                  ttcattcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCATGATTGCTTTCTTCAAGGt                                        <  1:2018233/68‑1 (MQ=255)

                  ttcattcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCATGATTGCTTTCTTCAAGGt                                        <  1:1547397/68‑1 (MQ=255)

                  ttcattcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCATGATTGCTTTCTTCAAGGt                                        <  1:2527401/68‑1 (MQ=255)

                  ttcattcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCATGATTGCTTTCTTCAAGGt                                        <  1:2486947/68‑1 (MQ=255)

                  ttcattcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCATGATTGCTTTCTTCAAGGt                                        <  1:2437302/68‑1 (MQ=255)

                  ttcattcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCATGATTGCTTTCTTCAAGGt                                        <  1:1165933/68‑1 (MQ=255)

                  ttcattcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCATGATTGCTTTCTTCAAGGt                                        <  1:1547453/68‑1 (MQ=255)

                  ttcattcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCATGATTGCTTTCTTCAAGGt                                        <  1:242285/68‑1 (MQ=255)

                  ttcattcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCATGATTGCTTTCTTCAAGGt                                        <  1:497972/68‑1 (MQ=255)

                  ttcattcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCATGATTGCTTTCTTCAAGGt                                        <  1:2143003/68‑1 (MQ=255)

                  ttcattcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCATGATTGCTTTCTTCAAGGt                                        <  1:1584012/68‑1 (MQ=255)

                  ttcattcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCATGATTGCTTTCTTCAAGGt                                        <  1:1313909/68‑1 (MQ=255)

                  ttcattcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCATGATTGCTTTCTTCAAGGt                                        <  1:2391314/68‑1 (MQ=255)

                  ttcattcaACTGGAGGTTGATCGCCGAGGTTGGGGCGTTAACCCAGCCATGATTGCTTTCTTCAAGGt                                        <  1:79358/68‑1 (MQ=255)

                  ttcattcaACTGGAGGTTGATCGCAGAGGTTGGGTCGTTAACCCAGCCATGATTGCTTTCTTCAAGGt                                        <  1:572746/68‑1 (MQ=255)

                   tcattcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTAACCCAGCCATGATTGCTTTCTTCAAGGt                                        <  1:1518746/67‑1 (MQ=255)

                      ttcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGc                                    <  1:926497/68‑1 (MQ=255)

                      ttcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGc                                    <  1:1582902/68‑1 (MQ=255)

                      ttcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGc                                    <  1:1089756/68‑1 (MQ=255)

                      ttcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGc                                    <  1:2521427/68‑1 (MQ=255)

                      ttcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGc                                    <  1:1572599/68‑1 (MQ=255)

                      ttcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGc                                    <  1:2248999/68‑1 (MQ=255)

                      ttcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGc                                    <  1:418209/68‑1 (MQ=255)

                      ttcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGc                                    <  1:2607730/68‑1 (MQ=255)

                      ttcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGc                                    <  1:2843017/68‑1 (MQ=255)

                        caACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCaa                                  >  1:2610202/1‑68 (MQ=255)

                                ggTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCAt                         <  1:934192/69‑1 (MQ=255)

                                ggTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCAt                         <  1:1361067/69‑1 (MQ=255)

                                   tGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGa                       >  1:1418049/1‑68 (MQ=255)

                                   tGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGa                       >  1:1610190/1‑68 (MQ=255)

                                   tGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGa                       >  1:872526/1‑68 (MQ=255)

                                   tGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGa                       >  1:2865968/1‑68 (MQ=255)

                                   tGATAGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGa                       >  1:2967969/1‑68 (MQ=255)

                                              gTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGttt             >  1:238947/1‑67 (MQ=255)

                                              gTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGttt             >  1:3115398/1‑67 (MQ=255)

                                              gTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGttt             >  1:1839559/1‑67 (MQ=255)

                                                 gggTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTca           >  1:662229/1‑66 (MQ=255)

                                                 gggTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTca           >  1:1881262/1‑66 (MQ=255)

                                                 gggTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTca           >  1:1412857/1‑66 (MQ=255)

                                                 gggTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTca           >  1:92363/1‑66 (MQ=255)

                                                 gggTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTca           >  1:1554553/1‑66 (MQ=255)

                                                 gggTCGTTTACCCAGCCATGATTGATTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTca           >  1:364639/1‑66 (MQ=255)

                                                    tCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACagag      >  1:2885676/1‑68 (MQ=255)

                                                    tCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAgaga     >  1:825030/1‑69 (MQ=255)

                                                    tCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAgaga     >  1:1302769/1‑69 (MQ=255)

                                                    tCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAgaga     >  1:1210376/1‑69 (MQ=255)

                                                    tCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAgaga     >  1:101793/1‑69 (MQ=255)

                                                    tCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAgaga     >  1:1431497/1‑69 (MQ=255)

                                                    tCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAgaga     >  1:1860894/1‑69 (MQ=255)

                                                    tCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAgaga     >  1:3235334/1‑69 (MQ=255)

                                                         tACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAAc  >  1:2332777/1‑67 (MQ=255)

                                                         tACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAAc  >  1:781012/1‑67 (MQ=255)

                                                         tACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAAc  >  1:2972708/1‑67 (MQ=255)

                                                         tACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAAc  >  1:1261306/1‑67 (MQ=255)

                                                         tACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAAc  >  1:257860/1‑67 (MQ=255)

                                                         tACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAAc  >  1:2606361/1‑67 (MQ=255)

                                                         tACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAAc  >  1:2862834/1‑67 (MQ=255)

                                                         tACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAAc  >  1:2643657/1‑67 (MQ=255)

                                                         tACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAAc  >  1:1143063/1‑67 (MQ=255)

                                                         tACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAAc  >  1:2804574/1‑67 (MQ=255)

CGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAAC  >  minE/309010‑309133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`