| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 310,164 | C→T | 47.5% | L903L (CTG→CTA) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 310,164 | 0 | C | T | 47.5% | ‑0.0 / 31.4 | 40 | L903L (CTG→CTA) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (17/4); new base T (5/14); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
TCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCA > minE/310102‑310210 | tCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATa > 1:1238437/1‑69 (MQ=255)tCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATa > 1:559166/1‑69 (MQ=255)tCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATa > 1:3158245/1‑69 (MQ=255)tCATTGGTCAGGCCACGGAAGGTGGCAGC‑‑‑CAACGCACCGATAACCCCCAGCGGAACGACCAGCATaa > 1:967860/1‑67 (MQ=37) ttGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACTAGCATAACg < 1:3206244/69‑1 (MQ=255) tGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACTAGCATAACg < 1:1168322/68‑1 (MQ=255) tGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACTAGCATAACg < 1:679186/68‑1 (MQ=255) tGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACTAGCATAACg < 1:673985/68‑1 (MQ=255) tGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACTAGCATAACg < 1:3203777/68‑1 (MQ=255) tGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACTAGCATAACg < 1:3051728/68‑1 (MQ=255) tGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACTAGCATAACg < 1:3027354/68‑1 (MQ=255) tGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACTAGCATAACg < 1:295080/68‑1 (MQ=255) tGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACTAGCATAACg < 1:2719557/68‑1 (MQ=255) tGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACTAGCATAACg < 1:1506616/68‑1 (MQ=255) tGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACTAGCATAACg < 1:198675/68‑1 (MQ=255) tGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACTAGCATAACg < 1:2149644/68‑1 (MQ=255) ggTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACTAGCATAACg < 1:171404/67‑1 (MQ=255) ggTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACgg > 1:683741/1‑68 (MQ=255) tCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACTAGCATAACg < 1:3074968/65‑1 (MQ=255) cAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGaa > 1:2464692/1‑69 (MQ=255) cAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGaa > 1:90525/1‑69 (MQ=255) cAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGaa > 1:904266/1‑69 (MQ=255) cAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGaa > 1:2055742/1‑69 (MQ=255) gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACTAGCATAACGGAGAACgg > 1:3232436/1‑69 (MQ=255) gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACTAGCATAACGGAGAACgg > 1:3331191/1‑69 (MQ=255) gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACTAGCATAACGGAGAACgg > 1:1219060/1‑69 (MQ=255) gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACTAGCATAACGGAGAACgg > 1:1014404/1‑69 (MQ=255) gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCACCAGCGGAACGACTAGCATAACGGAGAACgg > 1:2990478/1‑69 (MQ=255) ccACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGa > 1:1458125/1‑69 (MQ=255) ccACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGa > 1:1741860/1‑69 (MQ=255) ccACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGa < 1:2114293/69‑1 (MQ=255) ccACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGa < 1:2510255/69‑1 (MQ=255) cACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGa < 1:1799798/68‑1 (MQ=255) tGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGc > 1:3147406/1‑69 (MQ=255) tGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGc > 1:556363/1‑69 (MQ=255) ggCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGct < 1:847059/69‑1 (MQ=255) gATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCa > 1:309231/1‑68 (MQ=255) gATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCa > 1:1451410/1‑68 (MQ=255) gATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCa > 1:2819440/1‑68 (MQ=255) gATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCCCCa > 1:2244634/1‑68 (MQ=255) | TCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCA > minE/310102‑310210 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |