| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,087 | G→T | 60.9% | H596N (CAT→AAT) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,087 | 0 | G | T | 60.9% | 27.1 / 59.6 | 64 | H596N (CAT→AAT) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base G (21/4); new base T (23/16); total (44/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.26e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAAAGCCGAAGCCGTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTT > minE/311020‑311119 | gCAAAGCCGAAGCCGTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATg < 1:823691/68‑1 (MQ=255)gCAAAGCCGAAGCCGTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATg < 1:2568299/68‑1 (MQ=255) aagccgaagccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGtt > 1:1924022/1‑69 (MQ=255) agccgTTAACGGCGAACACCGAGTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:3109618/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:2108962/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:1169078/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:2136779/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:2461267/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:2648015/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:2676983/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:2730237/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:2774636/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:3048777/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:307403/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:3374087/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:3417124/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:451913/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:452682/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:48193/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:549341/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:580259/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:866652/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:906684/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:1975992/1‑68 (MQ=255) agccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATTCGTTACCTCa > 1:126437/1‑68 (MQ=255) agccgATAACGGCGAACACCGACTCAACGTTGTTCTATTCTTTGCTCAGATAGTAATTCGTTACCTCa > 1:2106779/1‑68 (MQ=255) gCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGt > 1:1319518/1‑50 (MQ=255) gCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGt > 1:3261970/1‑50 (MQ=255) gCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGt > 1:1953871/1‑50 (MQ=255) gCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGt > 1:2341806/1‑50 (MQ=255) gCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGt > 1:2052981/1‑50 (MQ=255) ccGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGc < 1:2125137/56‑1 (MQ=255) ccGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGc < 1:1549019/56‑1 (MQ=255) ccGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGc < 1:1619397/56‑1 (MQ=255) ccGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTa > 1:462114/1‑68 (MQ=255) ccGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTAc > 1:809778/1‑69 (MQ=255) ccGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTAc > 1:773932/1‑69 (MQ=255) ccGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTAc > 1:208300/1‑69 (MQ=255) ccGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTAc > 1:1039075/1‑69 (MQ=255) ccGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTAc > 1:1353359/1‑69 (MQ=255) ccGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTAc > 1:1407571/1‑69 (MQ=255) ccGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTAc > 1:1459490/1‑69 (MQ=255) ccGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTAc > 1:3180752/1‑69 (MQ=255) ccGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTAc > 1:2871096/1‑69 (MQ=255) ccGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTAc > 1:2164282/1‑69 (MQ=255) ccGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTAc > 1:2839009/1‑69 (MQ=255) ccGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTAc > 1:1621248/1‑69 (MQ=255) ccGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTATCTGTGTAc > 1:3420282/1‑69 (MQ=255) ccGACTCAACGGTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTAc > 1:2854097/1‑69 (MQ=255) aCTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGtt < 1:431832/69‑1 (MQ=255) ctttGGTCAGATAGTAATTCGTTACCTCATTGAGCACTTTCtgcg < 1:1981879/45‑3 (MQ=255) ctttGGTCAGATAGTAATTCGTTACCTCATTGAGCACTTTCtgcg < 1:2861774/45‑3 (MQ=255) ctttGGTCAGATAGTAATTCGTTACCTCATTGAGCACTTTCtgcg < 1:1296834/45‑3 (MQ=255) ctttGGTCAGATAGTAATTCGTTACCTCATTGAGCACTTTCtgcg < 1:2311881/45‑3 (MQ=255) ctttGGTCAGATAGTAATTCGTTACCTCATTGAGCACTTTCtgcg < 1:2418949/45‑3 (MQ=255) ctttGGTCAGATAGTAATTCGTTACCTCATTGAGCACTTTCtgcg < 1:2473691/45‑3 (MQ=255) ctttGGTCAGATAGTAATTCGTTACCTCATTGAGCACTTTCtgcg < 1:1326528/45‑3 (MQ=255) ctttGGTCAGATAGTAATTCGTTACCTCATTGAGCACTTTCtgcg < 1:2530714/45‑3 (MQ=255) ctttGGTCAGATAGTAATTCGTTACCTCATTGAGCACTTTCtgcg < 1:1770521/45‑3 (MQ=255) ctttGGTCAGATAGTAATTCGTTACCTCATTGAGCACTTTCtgcg < 1:1754222/45‑3 (MQ=255) ctttGGTCAGATAGTAATTCGTTACCTCATTGAGCACTTTCtgcg < 1:14186/45‑3 (MQ=255) ctttGGTCAGATAGTAATTCGTTACCTCATTGAGCACTTTCtgcg < 1:40179/45‑3 (MQ=255) ctttGGTCAGATAGTAATTCGTTACCTCATTGAGCACTTTCtgcg < 1:2698969/45‑3 (MQ=255) ctttGGTCAGATAGTAATTCGTTACCTCATTGAGCACTTTCtgcg < 1:1733324/45‑3 (MQ=255) ctttGGTCAGATAGTAATTCGTTACCTCATTGAGCACTTTCtgcg < 1:1553748/45‑3 (MQ=255) ctttGGTCAGATAGTAATTCGTTACCTCATTGAGCACTTTCtgcg < 1:1590362/45‑3 (MQ=255) | GCAAAGCCGAAGCCGTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTT > minE/311020‑311119 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |