| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 312,078 | G→C | 51.5% | V265V (GTC→GTG) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 312,078 | 0 | G | C | 51.5% | ‑5.1 / 80.5 | 66 | V265V (GTC→GTG) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base G (17/15); new base C (19/15); total (36/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
CCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTC > minE/312025‑312143 | ccGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACgcagcag < 1:1576463/68‑1 (MQ=255)ccGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACgcagcag < 1:2100040/68‑1 (MQ=255) aaaCTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCAcgc > 1:3373956/1‑68 (MQ=255) aaaCTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCAcg > 1:2173572/1‑67 (MQ=255) ctctGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGa < 1:2103654/69‑1 (MQ=255) ctctGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGa < 1:2318535/69‑1 (MQ=255) ctctGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGa < 1:1677583/69‑1 (MQ=255) ctctGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGa < 1:139572/69‑1 (MQ=255) ctctGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGa < 1:3010153/69‑1 (MQ=255) ctctGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGa < 1:3228847/69‑1 (MQ=255) ctctGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGa < 1:1285871/69‑1 (MQ=255) ctctGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGGCACGCAGCAGCACGCGGGa < 1:471545/69‑1 (MQ=255) gTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAAcc > 1:312660/1‑54 (MQ=255) gTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACt > 1:3331587/1‑67 (MQ=255) gTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACt > 1:1839509/1‑67 (MQ=255) gTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACt > 1:2393231/1‑67 (MQ=255) gTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACt > 1:1653997/1‑67 (MQ=255) gTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCATGATTCACt > 1:1573893/1‑67 (MQ=255) ctcACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTc > 1:1800483/1‑67 (MQ=255) caccCAGCTCAATTTTCGCCACATCACGCAGTAGCACGCGGGAACCATGCTGATTCACTTTcagcag < 1:3233701/67‑1 (MQ=255) caccCAGCTCAATTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTcagtag < 1:2544803/67‑1 (MQ=37) caccCAGCTCAATTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTcagcag < 1:206128/67‑1 (MQ=38) caccCAGCTCAATTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTcagcag < 1:2526230/67‑1 (MQ=38) caccCAGCTCAATTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTcagcag < 1:1110998/67‑1 (MQ=38) caccCAGCTCAATTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTcagcag < 1:2762564/67‑1 (MQ=38) caccCAGCTCAATTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTcagcag < 1:624887/67‑1 (MQ=38) caccCAGCTCAATTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTcagcag < 1:1416420/67‑1 (MQ=38) caccCAGCTCAATTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTcagcag < 1:1373969/67‑1 (MQ=38) caccCAGCTCAATTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTcagcag < 1:1378122/67‑1 (MQ=38) caccCAGCTCAATTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTcagcag < 1:1352721/67‑1 (MQ=38) caccCAGCTCAATTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTcagcag < 1:1294240/67‑1 (MQ=38) caccCAGCTCAATTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTcagcag < 1:1637512/67‑1 (MQ=38) caccCAGCTCAATTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTcagcag < 1:1274927/67‑1 (MQ=38) caccCAGCTCAATTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTcagcag < 1:1922523/67‑1 (MQ=38) tCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCg < 1:3238717/69‑1 (MQ=255) tCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCg < 1:2736906/69‑1 (MQ=255) aaTTTTCGCCACATCGCGCAGTAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGa > 1:2782144/1‑68 (MQ=25) aaTTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTg > 1:2254960/1‑42 (MQ=21) aaTTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTg > 1:2588721/1‑64 (MQ=255) aaTTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGaa > 1:443847/1‑69 (MQ=25) aaTTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGaa > 1:33225/1‑69 (MQ=25) aaTTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGaa > 1:2943572/1‑69 (MQ=25) aaTTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGaa > 1:3250340/1‑69 (MQ=25) aaTTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGaa > 1:446922/1‑69 (MQ=25) aaTTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGaa > 1:1135944/1‑69 (MQ=25) aaTTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGaa > 1:553757/1‑69 (MQ=25) aaTTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGaa > 1:2873955/1‑69 (MQ=25) aaTTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGaa > 1:2012804/1‑69 (MQ=25) aaTTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGaa > 1:2710901/1‑69 (MQ=25) aaTTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGaa > 1:2589232/1‑69 (MQ=25) aaTTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGaa > 1:1641630/1‑69 (MQ=25) aaTTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGaa > 1:1889840/1‑69 (MQ=25) aaTTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGaa > 1:1719141/1‑69 (MQ=25) aaTTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGa > 1:2712103/1‑68 (MQ=25) aaTTTTCGCCACATCACGCAGTAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGa > 1:618401/1‑68 (MQ=25) tCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGaa < 1:724151/67‑1 (MQ=255) tCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGaa < 1:1099736/67‑1 (MQ=255) tCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAAct > 1:751086/1‑69 (MQ=255) tCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAAct > 1:57438/1‑69 (MQ=255) tCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAAct > 1:453572/1‑69 (MQ=255) tCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAAct > 1:1691461/1‑69 (MQ=255) tCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAAct > 1:2262731/1‑69 (MQ=255) tCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAAct > 1:3293436/1‑69 (MQ=255) tCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAAct > 1:2656429/1‑69 (MQ=255) tCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAAct > 1:1353835/1‑69 (MQ=255) tCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTAAGCAGGATTTTGCCGaa < 1:151253/67‑1 (MQ=255) cgcgACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTc > 1:1195304/1‑69 (MQ=255) | CCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTC > minE/312025‑312143 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |