| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 34,469 | A→G | 41.6% | V350A (GTG→GCG) | caiC ← | predicted crotonobetaine CoA ligase:carnitine CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 34,469 | 0 | A | G | 41.6% | 13.3 / 33.4 | 48 | V350A (GTG→GCG) | caiC | predicted crotonobetaine CoA ligase:carnitine CoA ligase |
| Reads supporting (aligned to +/- strand): ref base A (7/21); new base G (11/9); total (18/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.81e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.93e-01 | |||||||||||
ACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGG > minE/34402‑34532 | aCCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCa < 1:184136/68‑1 (MQ=255)aCCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCa < 1:839092/68‑1 (MQ=255)aCCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCa < 1:1692420/68‑1 (MQ=255)aCCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCAGCTTCGTAGCAAAACCCCa < 1:2054549/68‑1 (MQ=255)aCCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGACCTCCGCTTCGTAGCAAAACCCCa < 1:863281/68‑1 (MQ=255) gagctcACCAGCCGGGAGGGGACGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAAGcccgcccg < 1:316767/66‑1 (MQ=255) gagctcACCAGCCGGGAGGGGACGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAAGcccgcccg < 1:1883945/66‑1 (MQ=255) gagctcACCAGCCGGGAGGGGACGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAAGcccgcccg < 1:1413923/66‑1 (MQ=255) gagctcACCAGCCGGGAGGGGACGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAAGcccgcccg < 1:1146044/66‑1 (MQ=255) gagctcACCAGCCGGGAGGGGACGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAAGcccgcccg < 1:1133455/66‑1 (MQ=255) gagctcACCAGCCGGGAGGGGACGATTGTGATCGTCGCGGATCTCCGCTACGTAGCAAAAGcccgcccg < 1:759879/66‑1 (MQ=255) gATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCg < 1:1352944/69‑1 (MQ=255) gATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCg < 1:3116157/69‑1 (MQ=255) gATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCg < 1:279620/69‑1 (MQ=255) gATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCg < 1:1266188/69‑1 (MQ=255) agctcACCAGCCGGGAGGGGACGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAAGcccgcccg < 1:2985139/66‑1 (MQ=255) cGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCa < 1:1750914/53‑1 (MQ=255) gggAGCGGGCGATTGTGATCGTCGCGGATCTCAGCTTCGTAGCAAAACCCCACCCg < 1:1707255/56‑1 (MQ=255) ggAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGcc < 1:2438480/69‑1 (MQ=255) gAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGcc < 1:1331444/68‑1 (MQ=255) gCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAACCCCCACCCGACCAATCGACGGCCAGc > 1:2631419/1‑69 (MQ=255) gCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGc > 1:2772182/1‑69 (MQ=255) tgtgATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTAt > 1:3103770/1‑69 (MQ=255) tgtgATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTAt > 1:1673844/1‑69 (MQ=255) tgtgATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTAt > 1:688466/1‑69 (MQ=255) tgtgATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTAt > 1:2005865/1‑69 (MQ=255) aTCGTCGCGGATCTCCGCTTCGTAGCAAAAGCCCGCCCGACCAATCGACGGCCAGCGCCGTTTATCGcc < 1:698033/69‑1 (MQ=255) aTCGTCGCGGATCTCCGCTTCGTAGCAAAAGCCCGCCCGACCAATCGACGGCCAGCGCCGTTTATCGcc < 1:2156192/69‑1 (MQ=255) aTCGTCGCGGATCTCCGCTTCGTAGCAAAAGCCCGCCCGACCAATCGACGGCCAGCGCCGTTTATCGcc < 1:1238546/69‑1 (MQ=255) aTCGTCGCGGATCTCCGCTTCGTAGCAAAAGCCCGCCCGACCAATCGACGGCCAGCGCCGTTTATCGcc < 1:913686/69‑1 (MQ=255) aTCGTCGCGGATCTCCGCTTCGTAGCAAAAGCCCGCCCGACCAATCGACGGCCAGCGCCGTTTATCGcc < 1:2641760/69‑1 (MQ=255) aTCGTCGCGGATCTCCGCTTCGTAGCAAAAGCCCGCCCGACCAATCGACGGCCAGCGCCGTTTATCGcc < 1:534624/69‑1 (MQ=255) aTCGTCGCGGATCTCCGCTTCGTAGCAAAAGCCCGCCCGACCAATCGACGGCCAGCGCCGTTTATCGcc < 1:163544/69‑1 (MQ=255) aTCGTCGCGGATCTCCGCTTCGTAGCAAAAGCCCGCCCGACCAATCGACGGCCAGCGCCGTTTATCGcc < 1:3303440/69‑1 (MQ=255) tcgtcgCGGATCTCCGCTTCGTAGCAAAAGCCCGCCCGACCAATCGACGGCCAGCGCCGTTTATCGcc < 1:738024/68‑1 (MQ=255) cgtcgCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAg < 1:1791720/69‑1 (MQ=255) cgtcgCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAg < 1:2942670/69‑1 (MQ=255) gcgGATCTCCGCTTCGTAGCAAAAGCCCGCCCGACCAATCGACGGCCAGCGCCGTTTATCGCCa > 1:1441268/1‑64 (MQ=255) gcgGATCTCCGCTTCGTAGCAAAAGCCCGCCCGACCAATCGACGGCCAGCGCCGTTTATCGCCAgggct > 1:2083482/1‑66 (MQ=255) gcgGATCTCCGCTTCGTAGCAAAAGCCCGCCCGACCAATCGACGGCCAGCGCCGTTTATCGCCAGGGCg > 1:981021/1‑69 (MQ=255) gcgGATCTCCGCTTCGTAGCAAAAGCCCGCCCGACCAATCGACGGCCAGCGCCGTTTATCGCCAGGGCg > 1:3307254/1‑69 (MQ=255) gcgGATCTCCGCTTCGTAGCAAAAGCCCGCCCGACCAATCGACGGCCAGCGCCGTTTATCGCCAGGGCg > 1:39801/1‑69 (MQ=255) gcgGATCTCCGCTTCGTAGCAAAAGCCCGCCCGACCAATCGACGGCCAGCGCCGTTTATCGCCAGGGCg > 1:617291/1‑69 (MQ=255) gcgGATCTCCGCTTCGTAGCAAAAGCCCGCCCGACCAATCGACGGCCAGCGCCGTTTATCGCCAGGGCg > 1:2035523/1‑69 (MQ=255) gcgGATCTCCGCTTCGTAGCAAAAGCCCGCCCGACCAATCGACGGCCAGCGCCGTTTATCGCCAGGGCg > 1:2054424/1‑69 (MQ=255) gcgGATCTCCGCTTCGTAGCAAAAGCCCGCCCGACCAATCGACGGCCAGCGCCGTTTATCGCCAGGGCg > 1:2724977/1‑69 (MQ=255) gcgGATCTCCGCTTCGTAGCAAAAGCCCGCCCGACCAATCGACGGCCAGCGCCGTTTATCGCCAGGGCg > 1:1742977/1‑69 (MQ=255) gcgGATCTCCGCTTCGTAGCAAAAGCCCGCCCGACCAATCGACGGCCAGCGCCGTTTATCGCCAGGGCg > 1:3080975/1‑69 (MQ=255) gcgGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACg < 1:2150487/69‑1 (MQ=255) gcgGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACg < 1:858926/69‑1 (MQ=255) gcgGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACg < 1:754091/69‑1 (MQ=255) gcgGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACg < 1:1685169/69‑1 (MQ=255) cTTCGTAGCAAAACCCCACCCGACCAATCGACGTCCAGCGTCGTTTATCGCCAGGACGATCGCCGAt < 1:2875741/67‑1 (MQ=255) cTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGAt < 1:2991562/67‑1 (MQ=255) gCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCcac < 1:2233556/69‑1 (MQ=255) gCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCcac < 1:2113037/69‑1 (MQ=255) gCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCcac < 1:2704419/69‑1 (MQ=255) gCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCcac < 1:2087627/69‑1 (MQ=255) gCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCcac < 1:46654/69‑1 (MQ=255) gCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCcac < 1:1216865/69‑1 (MQ=255) aCCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATgg > 1:1046631/1‑69 (MQ=255) | ACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGG > minE/34402‑34532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |