| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 312,237 | G→A | 47.9% | A212A (GCC→GCT) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 312,237 | 0 | G | A | 47.9% | 4.8 / 82.0 | 94 | A212A (GCC→GCT) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base G (38/11); new base A (11/34); total (49/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.87e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01 | |||||||||||
GAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTT > minE/312175‑312293 | gagGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTc > 1:430561/1‑69 (MQ=255)gagGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTc > 1:3264492/1‑69 (MQ=255)gagGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTc > 1:2786224/1‑69 (MQ=255) ggCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCt > 1:2250333/1‑68 (MQ=255) ggCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTg > 1:1431834/1‑69 (MQ=255) ggCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTg > 1:835399/1‑69 (MQ=255) ggCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTg > 1:2164412/1‑69 (MQ=255) ggCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTg > 1:2181951/1‑69 (MQ=255) ggCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTg > 1:1499852/1‑69 (MQ=255) ggCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTg > 1:2259199/1‑69 (MQ=255) ggCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTg > 1:2696083/1‑69 (MQ=255) ggCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTg > 1:1320212/1‑69 (MQ=255) ggCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTg > 1:2878777/1‑69 (MQ=255) ggCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTg > 1:484426/1‑69 (MQ=255) ggCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTg > 1:3295229/1‑69 (MQ=255) aaGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTTACCCGCCGCAACCTGGGCGTTCTGCGCttt < 1:809402/69‑1 (MQ=255) aaGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCACCCTGGGCGTTCTGCGCttt > 1:1705023/1‑69 (MQ=255) aaGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCttt > 1:2232589/1‑69 (MQ=255) aaGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCttt < 1:2277039/69‑1 (MQ=255) aaGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCttt < 1:2255684/69‑1 (MQ=255) aaGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCttt > 1:1302374/1‑69 (MQ=255) tGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATg > 1:600790/1‑69 (MQ=255) tGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATg > 1:1897063/1‑69 (MQ=255) tGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATg > 1:3118144/1‑69 (MQ=255) tGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATg > 1:3252444/1‑69 (MQ=255) gCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGt > 1:2686746/1‑69 (MQ=255) gCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGt > 1:3324381/1‑69 (MQ=255) tttCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAAt > 1:2842782/1‑69 (MQ=255) aCCGGCGGCGTACCACCGAGCTGACCAGCTGCAACCTGAGCGTTc > 1:2855617/1‑45 (MQ=38) aCCGGCGGCGTACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGAc > 1:1694619/1‑68 (MQ=255) aCCGGCGGCGTACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGAc > 1:676711/1‑68 (MQ=255) aCCGGCGGCGTACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGAc > 1:696110/1‑68 (MQ=255) aCCGGCGGCGTACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGAc > 1:2452437/1‑68 (MQ=255) aCCGGCGGCGTACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGAc > 1:1248518/1‑68 (MQ=255) aCCGGCGGCGTACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGAc > 1:1816651/1‑68 (MQ=255) aCCGGCGGCGTACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGAc > 1:1844992/1‑68 (MQ=255) aCCGGCGGCGTACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGAc > 1:3344012/1‑68 (MQ=255) aCCGGCGGCGTACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGAc > 1:2105950/1‑68 (MQ=255) aCCGGCGGCGTACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGAc > 1:889310/1‑68 (MQ=255) ggcggcGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCa > 1:2179174/1‑69 (MQ=255) ggcggcGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCa > 1:1422177/1‑69 (MQ=255) tACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTa < 1:2178183/53‑1 (MQ=255) tACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTa < 1:1410470/53‑1 (MQ=255) tACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTa < 1:603127/53‑1 (MQ=255) tACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTa < 1:1957006/53‑1 (MQ=255) tACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTa < 1:2941744/53‑1 (MQ=255) tACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTa < 1:863649/53‑1 (MQ=255) tACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTa < 1:2375443/53‑1 (MQ=255) tACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTa < 1:145919/53‑1 (MQ=255) tACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTa < 1:1478262/53‑1 (MQ=255) tACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTa < 1:2112361/53‑1 (MQ=255) tACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTa < 1:3185091/53‑1 (MQ=255) tACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTa < 1:1604366/53‑1 (MQ=255) tACCACCGAGCTGACCAGCTGCAACCTGAGCGTTCTGTGCTTTGATGGCGGTa < 1:101998/53‑1 (MQ=255) ccaccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGt < 1:3017226/50‑1 (MQ=255) ccaccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGt < 1:3425509/50‑1 (MQ=255) ccaccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGt < 1:1647967/50‑1 (MQ=255) ccaccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGt < 1:2763983/50‑1 (MQ=255) ccaccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGt < 1:1736255/50‑1 (MQ=255) ccaccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTaa > 1:3361060/1‑52 (MQ=255) ccaccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGtt > 1:89342/1‑69 (MQ=255) ccaccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGtt > 1:366514/1‑69 (MQ=255) ccaccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGtt > 1:3324596/1‑69 (MQ=255) ccaccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGtt > 1:1992864/1‑69 (MQ=255) ccaccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGtt > 1:1114657/1‑69 (MQ=255) ccaccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGtt > 1:216625/1‑69 (MQ=255) ccaccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGtt > 1:3138870/1‑69 (MQ=255) ccaccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGtt > 1:2531617/1‑69 (MQ=255) ccaccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGt > 1:1507303/1‑68 (MQ=255) ccaccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGt > 1:1848539/1‑68 (MQ=255) ccgccgCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGt < 1:455265/69‑1 (MQ=255) ccgccgCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGt < 1:3401244/69‑1 (MQ=255) ccgccgCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGt < 1:1071213/69‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:910123/65‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:91304/65‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:949365/65‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:730190/65‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:565035/65‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:3312114/65‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:3051038/65‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:2942323/65‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:2756571/65‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:2659053/65‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:2592171/65‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:2502872/65‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:2306883/65‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:218778/65‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:2027481/65‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:2014678/65‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:164367/65‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:1623018/65‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:1554093/65‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:1324962/65‑1 (MQ=255) gcAACCTGAGCGTTCTGTGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt < 1:1236630/65‑1 (MQ=255) | GAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTT > minE/312175‑312293 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |