| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 312,408 | G→A | 32.0% | S155S (TCC→TCT) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 312,408 | 0 | G | A | 32.0% | 100.2 / 56.3 | 112 | S155S (TCC→TCT) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base G (44/32); new base A (22/14); total (66/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01 | |||||||||||
Only 100 of 116 total aligned reads displayed.
AACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGG > minE/312343‑312475 | acTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGgaga < 1:1021338/69‑1 (MQ=255) acTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGgaga < 1:3098469/69‑1 (MQ=255) acTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGgaga < 1:3108848/69‑1 (MQ=255) acTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGgaga < 1:666002/69‑1 (MQ=255) tgcaCATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGata < 1:2097078/66‑1 (MQ=255) tGATCATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGata < 1:3018692/69‑1 (MQ=255) tGAACATCACCCACGCCCGACGTACGGCTGATGTCATCTTTCATATTCGCCGCCACGTAGTCGGAGata < 1:719516/69‑1 (MQ=255) tGAACATCACCCACGCCCGACGTACGGCTGATGGGATCTTTCATATTCGCCGCCACGTAGTCGGAGata < 1:2952007/69‑1 (MQ=255) tGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCgg > 1:2451945/1‑64 (MQ=255) tGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGga > 1:271446/1‑65 (MQ=255) tGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGga > 1:1324545/1‑65 (MQ=255) tGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGga > 1:1041986/1‑65 (MQ=255) tGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGga > 1:2423894/1‑65 (MQ=255) tGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGga > 1:2067554/1‑65 (MQ=255) tGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGga > 1:356922/1‑65 (MQ=255) tGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGata < 1:3304806/69‑1 (MQ=255) tGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGata < 1:2049765/69‑1 (MQ=255) tGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGata < 1:1504999/69‑1 (MQ=255) tGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGata < 1:1671504/69‑1 (MQ=255) tGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGata < 1:1923256/69‑1 (MQ=255) tGAACATCACACACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGga > 1:894548/1‑65 (MQ=255) gAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGata < 1:3349233/68‑1 (MQ=255) cACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGc > 1:2972250/1‑67 (MQ=255) cACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGc > 1:1093744/1‑67 (MQ=255) cACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGc > 1:1431821/1‑67 (MQ=255) cACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGc > 1:2464795/1‑67 (MQ=255) cACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGc > 1:1500599/1‑67 (MQ=255) cACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGc > 1:2596620/1‑67 (MQ=255) cACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGc > 1:1925894/1‑67 (MQ=255) cACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGc > 1:2713298/1‑67 (MQ=255) cACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGc > 1:2793990/1‑67 (MQ=255) cACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGc > 1:557691/1‑67 (MQ=255) cACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGc > 1:1795465/1‑67 (MQ=255) aaCGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGc > 1:3001318/2‑67 (MQ=255) tACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCAt > 1:1067052/1‑69 (MQ=255) tACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCAt > 1:1884684/1‑69 (MQ=255) cGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCg > 1:2907463/1‑69 (MQ=255) cGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCg > 1:2983938/1‑69 (MQ=255) cGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCg > 1:3241804/1‑69 (MQ=255) cGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCg > 1:409328/1‑69 (MQ=255) tGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGata < 1:3064333/41‑1 (MQ=255) gATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGtt > 1:3412142/1‑69 (MQ=255) ggCATCTTTCATATTCGCTGCCACGTAGTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGAt < 1:457795/69‑1 (MQ=255) ggCATCTTTCATATTCGCTGCCACGTAGTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGAt < 1:3045190/69‑1 (MQ=255) ggCATCTTTCATATTCGCTGCCACGTAGTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGAt < 1:2789809/69‑1 (MQ=255) ggCATCTTTCATATTCGCTGCCACGTAGTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGAt < 1:1314023/69‑1 (MQ=255) ggCATCTTTCATATTCGCTGCCACGTAGTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGAt < 1:1910809/69‑1 (MQ=255) ggCATCTTTCATATTCGCTGCCACGTAGTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGAt < 1:3038233/69‑1 (MQ=255) ggCATCTTTCATATTCGCTGCCACGTAGTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGAt < 1:833466/69‑1 (MQ=255) ggCATCTTTCATATTCGCTGCCACGTAGTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGAt < 1:696878/69‑1 (MQ=255) ggCATCTTTCATATTCGCTGCCACGTAGTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGAt < 1:740981/69‑1 (MQ=255) ggCATCTTTCATATTCGCTGCCACGTAGTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGAt < 1:3353513/69‑1 (MQ=255) ggCATCTTTCATATTCGCTGCCACGTAGTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGAt < 1:1458861/69‑1 (MQ=255) ggCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGAt < 1:3241389/69‑1 (MQ=255) ggCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGAt < 1:1163593/69‑1 (MQ=255) ggCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGAt < 1:171000/69‑1 (MQ=255) ggCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGAt < 1:1140732/69‑1 (MQ=255) ggCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGAt < 1:3193320/69‑1 (MQ=255) ggCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGAGTTGAt < 1:1410/69‑1 (MQ=255) gCATCTTTCATATTGGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATa > 1:3076006/1‑69 (MQ=255) gCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGAt < 1:1721953/68‑1 (MQ=255) aTCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAAc < 1:3137172/69‑1 (MQ=255) aTCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAAc < 1:899255/69‑1 (MQ=255) aTCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAAc < 1:1830927/69‑1 (MQ=255) aTCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAAc < 1:1948759/69‑1 (MQ=255) aTCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAAc < 1:2065203/69‑1 (MQ=255) atatTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACa > 1:2807042/1‑69 (MQ=255) atatTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACa > 1:1676559/1‑69 (MQ=255) atatTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACa > 1:1651513/1‑69 (MQ=255) atatTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACa > 1:1907548/1‑69 (MQ=255) atatTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACa > 1:306076/1‑69 (MQ=255) atatTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACa > 1:1299484/1‑69 (MQ=255) atatTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACa > 1:2631810/1‑69 (MQ=255) ttCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGAt > 1:1342920/1‑56 (MQ=255) ttCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGAt > 1:2523029/1‑56 (MQ=255) ttCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGAt > 1:2339105/1‑56 (MQ=255) ttCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGAt > 1:151954/1‑56 (MQ=255) gTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTg > 1:531880/1‑40 (MQ=38) gTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGATAAcccc > 1:3127668/1‑48 (MQ=255) gTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGATAACCCCGACAACCa > 1:1028473/1‑56 (MQ=255) gTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGATAACCCCGACAACCa > 1:681247/1‑56 (MQ=255) gTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGATAACCCCGACAACCa > 1:694062/1‑56 (MQ=255) gTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGATAACCCCGACAACCa > 1:2827876/1‑56 (MQ=255) gTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGATAACCCCGACAACCa > 1:853114/1‑56 (MQ=255) gTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGATAACCCCGACAACCa > 1:703952/1‑56 (MQ=255) gTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGATAACCCCGACAACCa > 1:148316/1‑56 (MQ=255) gTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGATAACCCCGACAACCa > 1:761173/1‑56 (MQ=255) gTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGATAACCCCGACAACCa > 1:592852/1‑56 (MQ=255) gTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGATAACCCCGACAACCa > 1:503369/1‑56 (MQ=255) gTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGATAACCCCGACAACCa > 1:1863975/1‑56 (MQ=255) gTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGATAACCCCGACAACCa > 1:2079554/1‑56 (MQ=255) gTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGATAACCCCGACAACCa > 1:2103106/1‑56 (MQ=255) gTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGATAACCCCGACAACCa > 1:2214147/1‑56 (MQ=255) gTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGATAACCCCGACAACCa > 1:2396210/1‑56 (MQ=255) gTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGATAACCCCGACAACCa > 1:2532180/1‑56 (MQ=255) gTCAGAGATATCCTCCTGCGTCATAGTGCCATCGGTGTTGATAACCCCGACAACCa > 1:2602958/1‑56 (MQ=255) gTCAGAGATATACTCCTGCGTCATAGTGCCATCGGTGTTGATATCCCCGACAACCa > 1:280040/1‑56 (MQ=255) ggAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTgg > 1:571350/1‑68 (MQ=255) ggAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTgg > 1:842894/1‑68 (MQ=255) ggAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTgg > 1:2621321/1‑68 (MQ=255) | AACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGG > minE/312343‑312475 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |