Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 314,859 T→C 100% N210N (AAT→AAC)  acrR → DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE314,8590TC75.2% 14.8 / 0.9 16N210N (AAT→AAC) acrRDNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/4);  new base C (12/0);  total (12/4)
Fisher's exact test for biased strand distribution p-value = 5.49e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTC  >  minE/314809‑314918
                                                  |                                                           
tACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATaa                                           <  1:2512815/69‑1 (MQ=255)
tACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATaa                                           <  1:969039/69‑1 (MQ=255)
                        tATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAtttt                   <  1:2865687/69‑1 (MQ=255)
                               tgtgCCCCCCGCTTCGTAACCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAATTTTCCTGGa            >  1:1175826/1‑69 (MQ=255)
                               tgtgCCCCACGCTTCGTAACCCTGCCACTAACGAATAACCCTGAAt                                   >  1:654962/1‑46 (MQ=255)
                               tgtgCCCCACGCTTCGTAACCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAATTTTCCt               >  1:2852774/1‑66 (MQ=255)
                               tgtgCCCCACGCTTCGTAACCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAATTTTCCTgg             >  1:863432/1‑68 (MQ=255)
                               tgtgCCCCACGCTTCGTAACCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAATTTTCCTGGa            >  1:2043711/1‑69 (MQ=255)
                               tgtgCCCCACGCTTCGTAACCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAATTTTCCTGGa            >  1:246458/1‑69 (MQ=255)
                               tgtgCCCCACGCTTCGTAACCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAATTTTCCTGGa            >  1:2704841/1‑69 (MQ=255)
                               tgtgCCCCACGCTTCGTAACCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAATTTTCCTGGa            >  1:2810613/1‑69 (MQ=255)
                               tgtgCCCCACGCTTCGTAACCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAATTTTCCTGGa            >  1:3406758/1‑69 (MQ=255)
                               tgtgCCCCACGCTTCGTAACCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAATTTTCCTGGa            >  1:653848/1‑69 (MQ=255)
                               tgtgCCCCACGCTTCGTAACCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAATTTTCCTGGa            >  1:697805/1‑69 (MQ=255)
                               tgtgCCCCACGCTTCGTAACCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAATTTTCCTGGa            >  1:831639/1‑69 (MQ=255)
                                         gCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTtcgtc  <  1:1046274/69‑1 (MQ=255)
                                                  |                                                           
TACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTC  >  minE/314809‑314918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑