| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 318,020 | C→A | 18.3% | R1006S (CGT→AGT) | kefA → | fused mechanosensitive channel proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 318,020 | 0 | C | A | 18.3% | 202.4 / 28.3 | 98 | R1006S (CGT→AGT) | kefA | fused mechanosensitive channel proteins |
| Reads supporting (aligned to +/- strand): ref base C (53/27); new base A (13/5); total (66/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01 | |||||||||||
GTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGA > minE/317959‑318083 | gTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTTATCCGTCTCgg < 1:3408611/69‑1 (MQ=255)gTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCgg < 1:2824269/69‑1 (MQ=255)gTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCgg < 1:30068/69‑1 (MQ=255) tGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCg > 1:2096330/1‑68 (MQ=255) tGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCg > 1:246237/1‑68 (MQ=255) tGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGt > 1:2711941/1‑69 (MQ=255) tGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGt > 1:2952089/1‑69 (MQ=255) tGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGt > 1:3385996/1‑69 (MQ=255) aCCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGcc < 1:2805123/69‑1 (MQ=255) tGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGtctc > 1:1052792/1‑50 (MQ=255) tGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTcc > 1:1187303/1‑68 (MQ=255) tGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTcc > 1:79314/1‑68 (MQ=255) tGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCg > 1:3285628/1‑69 (MQ=255) tGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCg > 1:440128/1‑69 (MQ=255) tGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCg > 1:470243/1‑69 (MQ=255) tGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCg > 1:2797753/1‑69 (MQ=255) tGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCg > 1:860661/1‑69 (MQ=255) tGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCg > 1:2422422/1‑69 (MQ=255) tGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCg > 1:2399784/1‑69 (MQ=255) tGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCg > 1:983103/1‑69 (MQ=255) tGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCg > 1:1870321/1‑69 (MQ=255) tGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCg > 1:105508/1‑69 (MQ=255) tGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCg > 1:1058494/1‑69 (MQ=255) tGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCg > 1:1465617/1‑69 (MQ=255) cAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGCTc > 1:759444/1‑68 (MQ=255) cAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATc > 1:1488045/1‑68 (MQ=255) cAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATc > 1:766963/1‑68 (MQ=255) cAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATc > 1:1705665/1‑68 (MQ=255) cAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATc > 1:2419653/1‑68 (MQ=255) cAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATc > 1:1099054/1‑68 (MQ=255) cAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATc > 1:3011911/1‑68 (MQ=255) cAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATc > 1:1438541/1‑68 (MQ=255) cAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATc > 1:2239972/1‑68 (MQ=255) cGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAg < 1:2237404/69‑1 (MQ=255) gTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAg < 1:2714390/68‑1 (MQ=255) gTTGACTGACACTACTACGCGTCTGGTGATCAGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGt > 1:2906074/1‑69 (MQ=255) gTTGACTGACACTACTACGCGTCTGGTGATCAGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGt > 1:1664306/1‑69 (MQ=255) gTTGACTGACACTACTACGCGTCTGGTGATCAGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGt > 1:3141189/1‑69 (MQ=255) gTTGACTGACACTACTACGCGTCTGGTGATCAGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGt > 1:1293916/1‑69 (MQ=255) gTTGACTGACACTACTACGCGTCTGGTGATCAGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGt > 1:2813422/1‑69 (MQ=255) gTTGACTGACACTACTACGCGTCTGGTGATCAGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGt > 1:160606/1‑69 (MQ=255) gTTGACTGACACTACTACGCGTCTGGTGATCAGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGt > 1:3323189/1‑69 (MQ=255) gTTGACTGACACTACTACGCGTCTGGTGATCAGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGt > 1:3393341/1‑69 (MQ=255) gTTGACTGACACTACTACGCGTCTGGTGATCAGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGt > 1:1208380/1‑69 (MQ=255) gTTGACTGACACTACTACGCGTCTGGTGATCAGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGt > 1:369362/1‑69 (MQ=255) gTTGACTGACACTACTACGCGTCTGGTGATCAGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGt > 1:386917/1‑69 (MQ=255) gTTGACTGACACTACTACGCGTCTGGTGATCAGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGt > 1:533284/1‑69 (MQ=255) tgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTa < 1:3305630/68‑1 (MQ=255) tgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTa < 1:1875517/68‑1 (MQ=255) tgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTa < 1:3371527/68‑1 (MQ=255) tgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTa < 1:1325123/68‑1 (MQ=255) tgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTa < 1:28166/68‑1 (MQ=255) tgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTa < 1:1014403/68‑1 (MQ=255) tgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTa < 1:1562134/68‑1 (MQ=255) tgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTa < 1:2639433/68‑1 (MQ=255) ctactaCGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAgtgt < 1:2775049/69‑1 (MQ=255) ctactaCGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAgtgt < 1:2372144/69‑1 (MQ=255) ctactaCGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAgtgt < 1:2563559/69‑1 (MQ=255) ctactaCGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAgtgt < 1:3255049/69‑1 (MQ=255) tactacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGtt < 1:393057/69‑1 (MQ=255) tactacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGtt < 1:2406459/69‑1 (MQ=255) tactacGCGTCTGGTGATCAGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGtt < 1:2103086/69‑1 (MQ=255) tactacGCGTCTGGTGATCAGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGtt < 1:453513/69‑1 (MQ=255) tactacGCGTCTGGTGATCAGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGtt < 1:381387/69‑1 (MQ=255) actacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGaaa > 1:3101522/1‑52 (MQ=255) actacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGtt > 1:2813329/1‑68 (MQ=255) actacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTa > 1:2000654/1‑69 (MQ=255) actacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTa > 1:2089856/1‑69 (MQ=255) actacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTa > 1:537574/1‑69 (MQ=255) actacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTa > 1:3095041/1‑69 (MQ=255) actacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTa > 1:1847519/1‑69 (MQ=255) actacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTa > 1:972599/1‑69 (MQ=255) actacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTa > 1:804095/1‑69 (MQ=255) actacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTa > 1:2845106/1‑69 (MQ=255) actacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTa > 1:93945/1‑69 (MQ=255) actacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTa > 1:2257120/1‑69 (MQ=255) actacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTa > 1:2190660/1‑69 (MQ=255) actacGCGTCTGGTGATCAGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGtt < 1:815244/68‑1 (MQ=255) tacGCGTCTGGTGATCAGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGtt < 1:2254366/66‑1 (MQ=255) gcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTa < 1:3218217/56‑1 (MQ=255) gTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGgcggcg > 1:2627167/1‑69 (MQ=255) gTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGgcggcg > 1:982414/1‑69 (MQ=255) gTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGgcggcg > 1:2943948/1‑69 (MQ=255) gTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGgcggcg > 1:2614423/1‑69 (MQ=255) gTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGgcggcg > 1:3150697/1‑69 (MQ=255) gTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGgcggcg > 1:2608060/1‑69 (MQ=255) gTGATCAGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGgcggcg > 1:2616028/1‑69 (MQ=255) gATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAGgtgt < 1:74115/53‑1 (MQ=255) gATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAgtgt < 1:187903/53‑1 (MQ=255) gATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAgtgt < 1:1546825/53‑1 (MQ=255) gATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAgtgt < 1:1900032/53‑1 (MQ=255) gATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAgtgt < 1:1623182/53‑1 (MQ=255) aTCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGtt > 1:2259608/1‑53 (MQ=255) aTCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGGGa > 1:3077762/1‑67 (MQ=255) aTCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGa > 1:1064626/1‑67 (MQ=255) aTCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGa > 1:1285305/1‑67 (MQ=255) aTCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGa > 1:3097408/1‑67 (MQ=255) tCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTa < 1:2128075/45‑1 (MQ=255) | GTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGA > minE/317959‑318083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |