| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 320,261 | C→T | 100% | D127D (GAC→GAT) | apt → | adenine phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 320,261 | 0 | C | T | 78.7% | 13.6 / ‑0.7 | 14 | D127D (GAC→GAT) | apt | adenine phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (11/0); total (12/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.30e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTG > minE/320204‑320322 | gCTGGAAATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGATCTGCTGGCAAc > 1:1218770/1‑69 (MQ=255)gCTGGAAATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGATCTGCTGGCAAc > 1:1234301/1‑69 (MQ=255)gCTGGAAATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGATCTGCTGGCAAc > 1:1497605/1‑69 (MQ=255)gCTGGAAATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGATCTGCTGGCAAc > 1:1558154/1‑69 (MQ=255)gCTGGAAATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGATCTGCTGGCAAc > 1:2036913/1‑69 (MQ=255)gCTGGAAATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGATCTGCTGGCAAc > 1:2327226/1‑69 (MQ=255)gCTGGAAATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGATCTGCTGGCAAc > 1:2523923/1‑69 (MQ=255)gCTGGAAATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGATCTGCTGGCAAc > 1:2703687/1‑69 (MQ=255)gCTGGAAATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGATCTGCTGGCAAc > 1:343401/1‑69 (MQ=255)gCTGGAAATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGATCTGCTGGCAAc > 1:544822/1‑69 (MQ=255)gCTGGAAATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGATCTGCTGGCAAc > 1:571638/1‑69 (MQ=255) ttGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTAt > 1:1518823/1‑67 (MQ=255) aCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTa < 1:1429529/68‑1 (MQ=255) ggACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGgtggtg < 1:918847/68‑1 (MQ=255) | GCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTG > minE/320204‑320322 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |