| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 323,413 | G→A | 100% | E180E (GAG→GAA) | recR → | gap repair protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 323,413 | 0 | G | A | 80.6% | 64.4 / 8.7 | 46 | E180E (GAG→GAA) | recR | gap repair protein |
| Reads supporting (aligned to +/- strand): ref base G (0/9); new base A (5/32); total (5/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTA > minE/323349‑323478 | aGCTTTGTGCGCAATATTACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTgg < 1:2179910/69‑1 (MQ=255)aGCTTTGTGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTgg < 1:942790/69‑1 (MQ=255)aGCTTTGTGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTgg < 1:361657/69‑1 (MQ=255)aGCTTTGTGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTgg < 1:3406413/69‑1 (MQ=255)aGCTTTGTGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTgg < 1:559851/69‑1 (MQ=255)aGCTTTGTGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTgg < 1:2772653/69‑1 (MQ=255)aGCTTTGTGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTgg < 1:1798384/69‑1 (MQ=255)aGCTTTGTGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTgg < 1:3213493/69‑1 (MQ=255)aGCTTTGTGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTgg < 1:1164763/69‑1 (MQ=255) tgcgcAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTgg < 1:298722/61‑1 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:2777188/69‑2 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:2815439/69‑2 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:3259937/69‑2 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:3340692/69‑2 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:2613871/69‑2 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:3327785/69‑2 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:256539/69‑2 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:2546555/69‑2 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:247021/69‑2 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:3434965/69‑2 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:353743/69‑2 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:2323801/69‑2 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:230188/69‑2 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:2230557/69‑2 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:2216114/69‑2 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:79421/69‑2 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:1678427/69‑2 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:903662/69‑2 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:1452570/69‑2 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:1415768/69‑2 (MQ=255) cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:1289995/69‑2 (MQ=255) ccagccGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTcac < 1:3412976/69‑1 (MQ=255) agccGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGat < 1:1095693/51‑2 (MQ=255) cGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGt < 1:3411321/56‑1 (MQ=255) cGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGt < 1:2672865/56‑1 (MQ=255) cGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGt < 1:1896171/56‑1 (MQ=255) cGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGt < 1:744840/56‑1 (MQ=255) cGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGt < 1:1894710/56‑1 (MQ=255) cGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGt < 1:1528087/56‑1 (MQ=255) ggCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGt < 1:2754006/49‑1 (MQ=255) tCCGGTTGGCGGCGAGCTGGAAATGGGCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATaa < 1:719439/69‑1 (MQ=255) cgAACTGGAAATGGTCGATGGCACCACGCTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTa > 1:2764316/1‑69 (MQ=255) cgAACTGGAAATGGTCGATGGCACCACGCTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTa > 1:242358/1‑69 (MQ=255) cgAACTGGAAATGGTCGATGGCACCACGCTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTa > 1:2395826/1‑69 (MQ=255) cgAACTGGAAATGGTCGATGGCACCACGCTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTa > 1:1093683/1‑69 (MQ=255) cgAACTGGAAATGGTCGATGGCACCACGCTATCACACTCCCTTGCCGGGCGTCATAAGATTCGttttt > 1:231831/1‑68 (MQ=255) | AGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTA > minE/323349‑323478 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |