| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 323,771 | C→T | 50.0% | G61G (GGC→GGT) | htpG → | molecular chaperone HSP90 family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 323,771 | 0 | C | T | 50.0% | ‑2.0 / 53.0 | 60 | G61G (GGC→GGT) | htpG | molecular chaperone HSP90 family |
| Reads supporting (aligned to +/- strand): ref base C (20/10); new base T (19/11); total (39/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.35e-01 | |||||||||||
CCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACG > minE/323704‑323832 | ccTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCg < 1:3039988/69‑1 (MQ=255)ccTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCg < 1:2368393/69‑1 (MQ=255)ccTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCg < 1:2343785/69‑1 (MQ=255)ccTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCg < 1:2169860/69‑1 (MQ=255)ccTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCg < 1:1634815/69‑1 (MQ=255)ccTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCg < 1:511975/69‑1 (MQ=255) cTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCg < 1:2105823/68‑1 (MQ=255) tCCGCGCGCTTTCTAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATa < 1:3162715/69‑1 (MQ=255) tCCGCGCGCTTTCTAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATa < 1:2905506/69‑1 (MQ=255) tCCGCGCGCTTTCTAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATa < 1:2794486/69‑1 (MQ=255) tCCGCGCGCTTTCTAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATa < 1:220360/69‑1 (MQ=255) tCCGCGCGCTTTCTAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATa < 1:486067/69‑1 (MQ=255) tCCGCGCGCTTTCTAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATa < 1:1974789/69‑1 (MQ=255) tCCGCGCGCTTTCTAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATa < 1:1890602/69‑1 (MQ=255) tCCGCGCGCTTTCTAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATa < 1:16873/69‑1 (MQ=255) tCCGCGCGCTTTCTAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATa < 1:773479/69‑1 (MQ=255) tCCGCGCGCTTTCTAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGCCTCTTTCGATa < 1:2590564/69‑1 (MQ=255) tttctAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGAt > 1:1213780/3‑59 (MQ=255) tttctAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAg > 1:540227/3‑68 (MQ=255) tttctAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAg > 1:3150608/3‑68 (MQ=255) tttctAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAg > 1:3014836/3‑68 (MQ=255) tttctAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAg > 1:2920996/3‑68 (MQ=255) tttctAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAg > 1:3286877/3‑68 (MQ=255) tttctAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAg > 1:2691383/3‑68 (MQ=255) tttctAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAg > 1:2663176/3‑68 (MQ=255) tttctAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAg > 1:2658311/3‑68 (MQ=255) tttctAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAg > 1:1227794/3‑68 (MQ=255) tttctAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAg > 1:2318604/3‑68 (MQ=255) tttctAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAg > 1:551045/3‑68 (MQ=255) tttctAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAg > 1:2190357/3‑68 (MQ=255) tttctAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAg > 1:581491/3‑68 (MQ=255) tttctAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAg > 1:210505/3‑68 (MQ=255) tttctAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAg > 1:2032354/3‑68 (MQ=255) tttctAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAg > 1:567301/3‑68 (MQ=255) tttctAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAg > 1:1725471/3‑68 (MQ=255) tttctAACCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTAGTGTCTCTTTCGATAAAGACAAg > 1:3429597/3‑68 (MQ=255) ctctAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCg > 1:643241/1‑69 (MQ=255) ctctAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCg < 1:2468973/69‑1 (MQ=255) ctctAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCg > 1:2115077/1‑69 (MQ=255) ctctAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCg > 1:1100042/1‑69 (MQ=255) ctctAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCg < 1:1948892/69‑1 (MQ=255) tctAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCg < 1:223146/68‑1 (MQ=255) aaCCCGGACCTGTACGAAGGTGATGGTGAACTACGCGTTCGTGTCTCTTTCGATa < 1:1174087/55‑1 (MQ=255) tACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGCCAAGCGTACGCTGACCATCTcc > 1:2728936/1‑69 (MQ=255) tACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTcc > 1:3265303/1‑69 (MQ=255) tACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTcc > 1:399364/1‑69 (MQ=255) tACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTcc > 1:1113515/1‑69 (MQ=255) tACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTcc > 1:2856317/1‑69 (MQ=255) tACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTcc > 1:2799817/1‑69 (MQ=255) tACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTcc > 1:1906541/1‑69 (MQ=255) tACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTcc > 1:1738754/1‑69 (MQ=255) cGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCg > 1:1948099/1‑68 (MQ=255) cGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCg > 1:1690935/1‑68 (MQ=255) cGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCg > 1:1593239/1‑68 (MQ=255) cGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCg > 1:1251114/1‑68 (MQ=255) cGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCg > 1:942203/1‑68 (MQ=255) gTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACg > 1:3091112/1‑69 (MQ=255) gTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACg > 1:2241755/1‑69 (MQ=255) gTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACg > 1:1979062/1‑69 (MQ=255) gTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACg > 1:1600924/1‑69 (MQ=255) | CCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACG > minE/323704‑323832 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |