| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 37,432 | G→A | 29.8% | T211T (ACC→ACT) | caiA ← | crotonobetaine reductase subunit II, FAD‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 37,432 | 0 | G | A | 29.8% | 108.8 / 47.8 | 107 | T211T (ACC→ACT) | caiA | crotonobetaine reductase subunit II, FAD‑binding |
| Reads supporting (aligned to +/- strand): ref base G (31/44); new base A (8/24); total (39/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
TGACGCGGTTAAAGCCGTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGG > minE/37365‑37497 | tGACGCGGTTAAAGCCGTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAg > 1:2944513/1‑68 (MQ=255)tGACGCGGTTAAAGCCGTCACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAg > 1:101666/1‑68 (MQ=255) cgcgGTTAAAGCCGTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTg < 1:2327954/68‑1 (MQ=255) cgcgGTTAAAGCCGTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTg < 1:2189252/68‑1 (MQ=255) cgcgGTTAAAGCCGTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTg < 1:2165581/68‑1 (MQ=255) cgcgGTTAAAGCCGTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTg < 1:1601182/68‑1 (MQ=255) cgcgGTTAAAGCCGTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTg < 1:356851/68‑1 (MQ=255) cgcgGTTAAAGCCGTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTg < 1:1187588/68‑1 (MQ=255) cgcgGTTAAAGCCGTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTg < 1:565332/68‑1 (MQ=255) gcgGTTAAAGCCGTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTg < 1:1078281/67‑1 (MQ=255) gcgGTTAAAGCCGTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTg < 1:1047134/67‑1 (MQ=255) tAAAGCCGTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTcac > 1:426293/1‑69 (MQ=255) tAAAGCCGTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTcac > 1:2144217/1‑69 (MQ=255) tAAAGCCGTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTcac > 1:2782151/1‑69 (MQ=255) tAAAGCCGTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTcac > 1:1576008/1‑69 (MQ=255) tAAAGCCGTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTcac > 1:3154593/1‑69 (MQ=255) tAAAGCCCTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTcac > 1:1569869/1‑69 (MQ=255) gTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCt < 1:444650/69‑1 (MQ=255) gTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCt < 1:2761992/69‑1 (MQ=255) gTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCt < 1:253246/69‑1 (MQ=255) gTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCt < 1:2411992/69‑1 (MQ=255) gTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCt < 1:1632656/69‑1 (MQ=255) gTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCt < 1:3264355/69‑1 (MQ=255) gTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCt < 1:38832/69‑1 (MQ=255) gTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCt < 1:1301974/69‑1 (MQ=255) gTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCt < 1:1271663/69‑1 (MQ=255) gTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCt < 1:1267153/69‑1 (MQ=255) ttACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCt < 1:426277/68‑1 (MQ=255) ttACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCt < 1:286080/68‑1 (MQ=255) aCCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCTATc < 1:1631394/69‑1 (MQ=255) aCCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCTATc < 1:2755165/69‑1 (MQ=255) aCCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCTATc > 1:2131286/1‑69 (MQ=255) ttCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCTATCCa < 1:1043098/68‑1 (MQ=255) ttCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCTATCCa < 1:265709/68‑1 (MQ=255) ttCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCTATCCa < 1:9419/68‑1 (MQ=255) ttCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCTATCCa < 1:3090367/68‑1 (MQ=255) ttCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCTATCCa < 1:3249974/68‑1 (MQ=255) ttCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCTATCCa < 1:50306/68‑1 (MQ=255) tGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCt > 1:1655793/1‑69 (MQ=255) tGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCt > 1:1768941/1‑69 (MQ=255) tGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCt > 1:3017195/1‑69 (MQ=255) tGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCt > 1:2940352/1‑69 (MQ=255) tGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCt > 1:281711/1‑69 (MQ=255) tCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCTATc > 1:961263/1‑50 (MQ=255) tCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCtt > 1:1179893/1‑68 (MQ=255) tCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCtt > 1:2739384/1‑68 (MQ=255) tCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCtt > 1:2708981/1‑68 (MQ=255) tttCTCGTCCAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCttt > 1:2226269/1‑67 (MQ=255) tttCTCGTCCAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCttt > 1:750660/1‑67 (MQ=255) tttCTCGTCCAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCttt > 1:2911759/1‑67 (MQ=255) tttCTCGTCCAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCttt > 1:2420628/1‑67 (MQ=255) tttCTCGTCCAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCttt > 1:1603682/1‑67 (MQ=255) tttCTCGTCCAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCttt > 1:2770404/1‑67 (MQ=255) tttCTCGTCCAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCt > 1:840955/1‑65 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTGACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:1160932/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:3368508/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:3311848/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:328709/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:1385630/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:1127574/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:1351664/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:3225633/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:2290164/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:507732/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:896166/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:47650/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:1845813/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:1922517/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:1963319/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:2068281/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:2964624/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:2072325/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:786625/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:720287/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:755419/68‑1 (MQ=255) ccAGTTCCACGTCGTCAAAAGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTg < 1:61714/68‑1 (MQ=255) tCCACGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACt > 1:839973/1‑69 (MQ=255) tCCACGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACt > 1:1203361/1‑69 (MQ=255) tCCACGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACt > 1:2150578/1‑69 (MQ=255) tCCACGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACt > 1:5804/1‑69 (MQ=255) ccACGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGa > 1:3300899/1‑44 (MQ=255) ccACGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACt > 1:3424906/1‑68 (MQ=255) ccACGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACt > 1:324876/1‑68 (MQ=255) ccACGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACt > 1:288898/1‑68 (MQ=255) aCGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACttt < 1:1666485/68‑1 (MQ=255) aCGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACttt < 1:753535/68‑1 (MQ=255) aCGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACttt < 1:1590749/68‑1 (MQ=255) aCGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACttt < 1:2770957/68‑1 (MQ=255) aCGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACttt < 1:2673906/68‑1 (MQ=255) aCGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACttt < 1:2665358/68‑1 (MQ=255) aCGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACttt < 1:2559783/68‑1 (MQ=255) aCGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACttt < 1:2445440/68‑1 (MQ=255) aCGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACttt < 1:2420517/68‑1 (MQ=255) aCGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACttt < 1:2389828/68‑1 (MQ=255) cgtcgtCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACttt < 1:2332457/67‑1 (MQ=255) tcAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACttt < 1:2444106/62‑1 (MQ=255) aaaGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCgg > 1:1230968/1‑69 (MQ=255) aaaGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCgg > 1:2598965/1‑69 (MQ=255) aaaGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCgg > 1:2246102/1‑69 (MQ=255) aaaGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCgg > 1:2396291/1‑69 (MQ=255) | TGACGCGGTTAAAGCCGTTACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGGTGATTTCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGG > minE/37365‑37497 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |