| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 330,082 | G→A | 44.5% | T542T (ACC→ACT) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 330,082 | 0 | G | A | 44.5% | 16.3 / 57.1 | 74 | T542T (ACC→ACT) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (28/13); new base A (25/8); total (53/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01 | |||||||||||
CGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGC > minE/330028‑330144 | cGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAttt > 1:3328118/1‑68 (MQ=255)cGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTc > 1:200522/1‑69 (MQ=255)cGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTc > 1:1243440/1‑69 (MQ=255)cGTTTACCCCGTCACCACCACACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTc > 1:112643/1‑69 (MQ=255) tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg > 1:2873537/1‑68 (MQ=255) tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg > 1:675627/1‑68 (MQ=255) tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg > 1:988223/1‑68 (MQ=255) tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg > 1:1692395/1‑68 (MQ=255) tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg > 1:758217/1‑68 (MQ=255) tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg > 1:390972/1‑68 (MQ=255) tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg > 1:2641170/1‑68 (MQ=255) tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg > 1:1485952/1‑68 (MQ=255) tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg > 1:776971/1‑68 (MQ=255) tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg > 1:762698/1‑68 (MQ=255) tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg > 1:47616/1‑68 (MQ=255) tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg > 1:2731272/1‑68 (MQ=255) tACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGc > 1:2167659/1‑69 (MQ=255) cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc < 1:143188/69‑1 (MQ=255) cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc < 1:2568494/69‑1 (MQ=255) cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc < 1:138649/69‑1 (MQ=255) cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc < 1:1665315/69‑1 (MQ=255) cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc < 1:1272331/69‑1 (MQ=255) cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc < 1:2496977/69‑1 (MQ=255) gTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc < 1:75724/68‑1 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCAc > 1:1842343/1‑68 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:2730085/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:286429/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:3122226/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:701007/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:3034461/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:1975736/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:1131793/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:1142553/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:1574012/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:151846/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:1405140/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt < 1:73868/69‑1 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt < 1:158595/69‑1 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt < 1:1533423/69‑1 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt < 1:2144532/69‑1 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt < 1:3372513/69‑1 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt < 1:94123/69‑1 (MQ=255) ccTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt < 1:1526361/68‑1 (MQ=255) cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCAt > 1:191140/1‑63 (MQ=255) cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc > 1:630373/1‑69 (MQ=255) cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc > 1:1012258/1‑69 (MQ=255) cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc > 1:994613/1‑69 (MQ=255) cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc > 1:2950079/1‑69 (MQ=255) cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc > 1:2862289/1‑69 (MQ=255) cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc > 1:1493923/1‑69 (MQ=255) cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc > 1:15031/1‑69 (MQ=255) cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc > 1:2393704/1‑69 (MQ=255) cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc > 1:2845257/1‑69 (MQ=255) aCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt < 1:679639/64‑1 (MQ=255) ccgGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCg < 1:1228569/69‑1 (MQ=255) ccgGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCg < 1:3420763/69‑1 (MQ=255) ccAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCg < 1:347468/57‑1 (MQ=255) gcagTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGa < 1:2922782/69‑1 (MQ=255) gcagTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGa < 1:1046596/69‑1 (MQ=255) ccAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:3386311/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGAtt > 1:1638598/1‑46 (MQ=38) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGAtata > 1:2756057/1‑67 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:2616492/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:2543979/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:414879/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:201030/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:1986958/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:285523/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:130077/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:288622/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:991835/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:3080263/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTAGCACCACGTTCGGTGATATAc > 1:918687/1‑68 (MQ=255) aGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGc < 1:351258/68‑1 (MQ=255) | CGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGC > minE/330028‑330144 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |