Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 330,091 G→A 39.2% I539I (ATC→ATT ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE330,0910GA39.2% 43.0 / 54.6 84I539I (ATC→ATTybaLpredicted transporter with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base G (37/14);  new base A (25/8);  total (62/22)
Fisher's exact test for biased strand distribution p-value = 8.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

CGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCAT  >  minE/330028‑330158
                                                               |                                                                   
cGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAttt                                                                 >  1:3328118/1‑68 (MQ=255)
cGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTc                                                                >  1:200522/1‑69 (MQ=255)
cGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTc                                                                >  1:1243440/1‑69 (MQ=255)
cGTTTACCCCGTCACCACCACACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTc                                                                >  1:112643/1‑69 (MQ=255)
    tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg                                                             >  1:762698/1‑68 (MQ=255)
    tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg                                                             >  1:675627/1‑68 (MQ=255)
    tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg                                                             >  1:47616/1‑68 (MQ=255)
    tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg                                                             >  1:1692395/1‑68 (MQ=255)
    tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg                                                             >  1:390972/1‑68 (MQ=255)
    tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg                                                             >  1:2641170/1‑68 (MQ=255)
    tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg                                                             >  1:758217/1‑68 (MQ=255)
    tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg                                                             >  1:1485952/1‑68 (MQ=255)
    tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg                                                             >  1:2731272/1‑68 (MQ=255)
    tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg                                                             >  1:2873537/1‑68 (MQ=255)
    tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg                                                             >  1:988223/1‑68 (MQ=255)
    tACCTCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACg                                                             >  1:776971/1‑68 (MQ=255)
    tACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGc                                                            >  1:2167659/1‑69 (MQ=255)
         cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc                                                       <  1:138649/69‑1 (MQ=255)
         cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc                                                       <  1:2568494/69‑1 (MQ=255)
         cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc                                                       <  1:1272331/69‑1 (MQ=255)
         cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc                                                       <  1:2496977/69‑1 (MQ=255)
         cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc                                                       <  1:1665315/69‑1 (MQ=255)
         cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc                                                       <  1:143188/69‑1 (MQ=255)
          gTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc                                                       <  1:75724/68‑1 (MQ=255)
                accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCAc                                                 >  1:1842343/1‑68 (MQ=255)
                accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt                                                >  1:2730085/1‑69 (MQ=255)
                accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt                                                >  1:3122226/1‑69 (MQ=255)
                accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt                                                >  1:3034461/1‑69 (MQ=255)
                accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt                                                >  1:286429/1‑69 (MQ=255)
                accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt                                                >  1:701007/1‑69 (MQ=255)
                accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt                                                >  1:1131793/1‑69 (MQ=255)
                accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt                                                >  1:1975736/1‑69 (MQ=255)
                accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt                                                >  1:1142553/1‑69 (MQ=255)
                accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt                                                >  1:1405140/1‑69 (MQ=255)
                accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt                                                >  1:1574012/1‑69 (MQ=255)
                accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt                                                >  1:151846/1‑69 (MQ=255)
                accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt                                                <  1:2144532/69‑1 (MQ=255)
                accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt                                                <  1:94123/69‑1 (MQ=255)
                accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt                                                <  1:3372513/69‑1 (MQ=255)
                accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt                                                <  1:1533423/69‑1 (MQ=255)
                accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt                                                <  1:158595/69‑1 (MQ=255)
                accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt                                                <  1:73868/69‑1 (MQ=255)
                 ccTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt                                                <  1:1526361/68‑1 (MQ=255)
                  cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCAt                                                    >  1:191140/1‑63 (MQ=255)
                  cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc                                              >  1:630373/1‑69 (MQ=255)
                  cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc                                              >  1:2950079/1‑69 (MQ=255)
                  cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc                                              >  1:994613/1‑69 (MQ=255)
                  cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc                                              >  1:1012258/1‑69 (MQ=255)
                  cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc                                              >  1:1493923/1‑69 (MQ=255)
                  cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc                                              >  1:2393704/1‑69 (MQ=255)
                  cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc                                              >  1:15031/1‑69 (MQ=255)
                  cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc                                              >  1:2845257/1‑69 (MQ=255)
                  cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc                                              >  1:2862289/1‑69 (MQ=255)
                     aCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt                                                <  1:679639/64‑1 (MQ=255)
                          ccgGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCg                                      <  1:1228569/69‑1 (MQ=255)
                          ccgGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCg                                      <  1:3420763/69‑1 (MQ=255)
                                      ccAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCg                                      <  1:347468/57‑1 (MQ=255)
                                         gcagTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGa                       <  1:1046596/69‑1 (MQ=255)
                                         gcagTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGa                       <  1:2922782/69‑1 (MQ=255)
                                               ccAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc                  >  1:3386311/1‑68 (MQ=255)
                                               ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGAtt                                        >  1:1638598/1‑46 (MQ=38)
                                               ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGAtata                   >  1:2756057/1‑67 (MQ=255)
                                               ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc                  >  1:130077/1‑68 (MQ=255)
                                               ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc                  >  1:288622/1‑68 (MQ=255)
                                               ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc                  >  1:991835/1‑68 (MQ=255)
                                               ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc                  >  1:1986958/1‑68 (MQ=255)
                                               ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc                  >  1:201030/1‑68 (MQ=255)
                                               ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc                  >  1:285523/1‑68 (MQ=255)
                                               ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc                  >  1:3080263/1‑68 (MQ=255)
                                               ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc                  >  1:414879/1‑68 (MQ=255)
                                               ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc                  >  1:2616492/1‑68 (MQ=255)
                                               ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc                  >  1:2543979/1‑68 (MQ=255)
                                               ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTAGCACCACGTTCGGTGATATAc                  >  1:918687/1‑68 (MQ=255)
                                                 aGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGc                <  1:351258/68‑1 (MQ=255)
                                                       gTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc                  >  1:2448074/1‑60 (MQ=255)
                                                       gTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCGCTTCa         >  1:757166/1‑69 (MQ=255)
                                                       gTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCGCTTCa         >  1:753197/1‑69 (MQ=255)
                                                       gTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTc          >  1:736606/1‑68 (MQ=255)
                                                       gTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCa         >  1:2792814/1‑69 (MQ=255)
                                                       gTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCa         >  1:794266/1‑69 (MQ=255)
                                                       gTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCa         >  1:2420887/1‑69 (MQ=255)
                                                       gTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCa         >  1:2893188/1‑69 (MQ=255)
                                                       gTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCa         >  1:1005477/1‑69 (MQ=255)
                                                              cGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCAt  <  1:953057/69‑1 (MQ=255)
                                                               |                                                                   
CGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCAT  >  minE/330028‑330158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: