| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 330,103 | G→A | 43.8% | G535G (GGC→GGT) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 330,103 | 0 | G | A | 43.8% | 22.0 / 62.2 | 82 | G535G (GGC→GGT) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (32/14); new base A (13/23); total (45/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.59e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAT > minE/330037‑330161 | cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc < 1:2496977/69‑1 (MQ=255)cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc < 1:1665315/69‑1 (MQ=255)cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc < 1:1272331/69‑1 (MQ=255)cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc < 1:2568494/69‑1 (MQ=255)cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc < 1:138649/69‑1 (MQ=255)cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc < 1:143188/69‑1 (MQ=255) gTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc < 1:75724/68‑1 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCAc > 1:1842343/1‑68 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:1975736/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:286429/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:2730085/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:3034461/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:1574012/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:151846/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:3122226/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:1405140/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:701007/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:1142553/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACt > 1:1131793/1‑69 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt < 1:3372513/69‑1 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt < 1:2144532/69‑1 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt < 1:1533423/69‑1 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt < 1:158595/69‑1 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt < 1:73868/69‑1 (MQ=255) accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt < 1:94123/69‑1 (MQ=255) ccTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt < 1:1526361/68‑1 (MQ=255) cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCAt > 1:191140/1‑63 (MQ=255) cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc > 1:2862289/1‑69 (MQ=255) cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc > 1:2845257/1‑69 (MQ=255) cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc > 1:2950079/1‑69 (MQ=255) cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc > 1:1012258/1‑69 (MQ=255) cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc > 1:630373/1‑69 (MQ=255) cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc > 1:994613/1‑69 (MQ=255) cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc > 1:2393704/1‑69 (MQ=255) cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc > 1:1493923/1‑69 (MQ=255) cTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc > 1:15031/1‑69 (MQ=255) aCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATAGTACGGGCAATTTCACGCTCACCCATCACt < 1:679639/64‑1 (MQ=255) ccgGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCg < 1:3420763/69‑1 (MQ=255) ccgGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCg < 1:1228569/69‑1 (MQ=255) ccAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCg < 1:347468/57‑1 (MQ=255) gcagTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGa < 1:1046596/69‑1 (MQ=255) gcagTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGa < 1:2922782/69‑1 (MQ=255) ccAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:3386311/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGAtt > 1:1638598/1‑46 (MQ=38) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGAtata > 1:2756057/1‑67 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:991835/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:2543979/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:130077/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:285523/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:201030/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:1986958/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:288622/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:414879/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:2616492/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:3080263/1‑68 (MQ=255) ccAGCATAGTACGGGCAATTTCACGCTCACCCATCACTACCTGATTAGCACCACGTTCGGTGATATAc > 1:918687/1‑68 (MQ=255) aGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGc < 1:351258/68‑1 (MQ=255) gTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc > 1:2448074/1‑60 (MQ=255) gTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCGCTTCa > 1:757166/1‑69 (MQ=255) gTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCGCTTCa > 1:753197/1‑69 (MQ=255) gTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTc > 1:736606/1‑68 (MQ=255) gTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCa > 1:794266/1‑69 (MQ=255) gTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCa > 1:1005477/1‑69 (MQ=255) gTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCa > 1:2420887/1‑69 (MQ=255) gTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCa > 1:2893188/1‑69 (MQ=255) gTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCa > 1:2792814/1‑69 (MQ=255) cGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCAt < 1:953057/69‑1 (MQ=255) tttCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAt < 1:2746077/69‑1 (MQ=255) tttCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAt < 1:2493007/69‑1 (MQ=255) tttCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAt < 1:2299957/69‑1 (MQ=255) tttCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAt < 1:2227964/69‑1 (MQ=255) tttCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAt < 1:2676155/69‑1 (MQ=255) tttCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAt < 1:1191365/69‑1 (MQ=255) tttCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAt < 1:2688208/69‑1 (MQ=255) tttCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAt < 1:3284933/69‑1 (MQ=255) tttCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAt < 1:2024178/69‑1 (MQ=255) tttCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAt < 1:465577/69‑1 (MQ=255) tttCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAt < 1:1629143/69‑1 (MQ=255) tttCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAt < 1:3153606/69‑1 (MQ=255) tttCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGGGATATACGCCACTTCATCGTCATAAt < 1:1299079/69‑1 (MQ=255) ttCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAt < 1:20899/68‑1 (MQ=255) ttCACGCTCACCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAt < 1:2799345/68‑1 (MQ=255) | CGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAT > minE/330037‑330161 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |