Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 331,123 | T→C | 56.4% | K195K (AAA→AAG) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 331,123 | 0 | T | C | 56.4% | 24.6 / 66.0 | 87 | K195K (AAA→AAG) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
Reads supporting (aligned to +/- strand): ref base T (13/25); new base C (5/44); total (18/69) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.95e-03 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 |
CCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCAT > minE/331069‑331188 | ccACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGa < 1:378055/68‑1 (MQ=255) ccACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGa < 1:1880421/68‑1 (MQ=255) ccACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGa < 1:187748/68‑1 (MQ=255) ccACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGa < 1:1632980/68‑1 (MQ=255) ccACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGa < 1:2638145/68‑1 (MQ=255) ccACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGa < 1:2983888/68‑1 (MQ=255) ccACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGa < 1:3013669/68‑1 (MQ=255) ccACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGa < 1:2881382/68‑1 (MQ=255) ccACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGa < 1:1229393/68‑1 (MQ=255) cAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATc < 1:1340193/69‑1 (MQ=255) cAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATc < 1:2087805/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATGAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:1767707/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:2344647/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:204835/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:593805/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:551913/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:2225451/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:414332/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:617335/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:3369465/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:3075091/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:2670410/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:2715984/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:3365585/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:1994482/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:1321909/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:1438982/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:1287736/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:156661/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:1582551/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:1711497/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:1790646/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:721144/69‑1 (MQ=255) cAGACGGCGACCTACCAACATCATAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATc < 1:996039/69‑1 (MQ=255) aGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATc < 1:3404011/68‑1 (MQ=255) ggCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACt > 1:931784/1‑68 (MQ=255) ggCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACt > 1:2152242/1‑68 (MQ=255) ggCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACt > 1:2302986/1‑68 (MQ=255) ggCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACt > 1:2322369/1‑68 (MQ=255) ggCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACt > 1:1543855/1‑68 (MQ=255) ggCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACt > 1:3225150/1‑68 (MQ=255) ggCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACt > 1:818182/1‑68 (MQ=255) ggCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACt > 1:1321650/1‑68 (MQ=255) tAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATCg > 1:1061900/1‑47 (MQ=255) tAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCca > 1:2895158/1‑69 (MQ=255) tAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCca > 1:700023/1‑69 (MQ=255) tAATGGCGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCca > 1:1653105/1‑69 (MQ=255) tAATGGAGATAAATGCGATCACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCca > 1:1400822/1‑69 (MQ=255) aaTGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCcac < 1:824046/69‑1 (MQ=255) aaTGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCcac < 1:75132/69‑1 (MQ=255) aaTGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCcac < 1:3100918/69‑1 (MQ=255) aaTGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCcac < 1:447890/69‑1 (MQ=255) aaTGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCcac < 1:2683791/69‑1 (MQ=255) aaTGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCcac < 1:2468192/69‑1 (MQ=255) aaTGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCcac < 1:2926565/69‑1 (MQ=255) aaTGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCcac < 1:2469121/69‑1 (MQ=255) aaTGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCcac < 1:1494948/69‑1 (MQ=255) aTGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCcac < 1:2928831/68‑1 (MQ=255) aTGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCcac < 1:86912/68‑1 (MQ=255) aTGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCcac < 1:1446152/68‑1 (MQ=255) tGCGATCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTc > 1:485887/1‑69 (MQ=255) tGCGATCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTc > 1:788194/1‑69 (MQ=255) aTCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcat < 1:3286989/68‑1 (MQ=255) cACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat > 1:3254932/1‑69 (MQ=255) cACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat > 1:1314196/1‑69 (MQ=255) cACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatca > 1:1218473/1‑68 (MQ=255) cACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:2015822/69‑1 (MQ=255) cACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:651538/69‑1 (MQ=255) cACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:671473/69‑1 (MQ=255) cACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:1116554/69‑1 (MQ=255) cACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:2010177/69‑1 (MQ=255) cACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:182579/69‑1 (MQ=255) cACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:742875/69‑1 (MQ=255) cACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:2947553/69‑1 (MQ=255) cACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:2142620/69‑1 (MQ=255) cACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:2147329/69‑1 (MQ=255) cACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:545052/69‑1 (MQ=255) cACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:2364354/69‑1 (MQ=255) cACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:2392137/69‑1 (MQ=255) cACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:392153/69‑1 (MQ=255) cACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:2514452/69‑1 (MQ=255) cACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:2786443/69‑1 (MQ=255) cACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:3194646/69‑1 (MQ=255) cACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:3191902/69‑1 (MQ=255) cACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:3079970/69‑1 (MQ=255) cACCTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:3069133/69‑1 (MQ=255) cACCTTGCCGATGGTGATCCCCATATAGACTGAAAGAGTGGCAAAGCCCACATCGCCCTGTTCcatcat < 1:2910134/69‑1 (MQ=255) | CCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCAT > minE/331069‑331188 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |