BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	38,629	A→G	32.7%	Y327Y (TAT→TAC)	caiT ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	38,629	0	A	G	32.7%	90.9 / 61.0	113	Y327Y (TAT→TAC)	caiT	predicted transporter
Reads supporting (aligned to +/- strand): ref base A (27/49); new base G (17/20); total (44/69)
Fisher's exact test for biased strand distribution p-value = 3.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

TCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGG  >  minE/38573‑38693

tCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATcacccac                                                      <  1:2807508/69‑1 (MQ=255)

tCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATcacccac                                                      <  1:3154454/69‑1 (MQ=255)

tCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATcacccac                                                      <  1:331197/69‑1 (MQ=255)

tCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATcacccac                                                      <  1:349925/69‑1 (MQ=255)

       caGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGccc                                               <  1:2071448/69‑1 (MQ=255)

       caGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGccc                                               <  1:2717203/69‑1 (MQ=255)

       caGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGccc                                               <  1:3005197/69‑1 (MQ=255)

       caGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGccc                                               <  1:2436844/69‑1 (MQ=255)

       caGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGccc                                               <  1:2298604/69‑1 (MQ=255)

       caGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGccc                                               <  1:3261894/69‑1 (MQ=255)

       caGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGccc                                               <  1:62756/69‑1 (MQ=255)

       caGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGccc                                               <  1:1494268/69‑1 (MQ=255)

       caGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGccc                                               <  1:1207922/69‑1 (MQ=255)

         gTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATATCATAAATCACCCACCATGccc                                               <  1:367754/67‑1 (MQ=255)

           aCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATa                                                                <  1:922281/48‑1 (MQ=255)

           aCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATa                                                                <  1:2114368/48‑1 (MQ=255)

           aCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATa                                                                <  1:2605935/48‑1 (MQ=255)

           aCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATa                                                                <  1:3131223/48‑1 (MQ=255)

           aCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATa                                                                <  1:3342175/48‑1 (MQ=255)

           aCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATa                                                                <  1:3161370/48‑1 (MQ=255)

            cGACCACGGGAGATGCGGGTGAGGAAGATACTCATATGGATAGCGTAAATCACCCACCATGCCCAGTAg                                          <  1:2341587/69‑1 (MQ=255)

            cGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAg                                          <  1:1444786/69‑1 (MQ=255)

            cGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAg                                          <  1:668/69‑1 (MQ=255)

            cGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAg                                          <  1:2372211/69‑1 (MQ=255)

            cGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAg                                          <  1:645755/69‑1 (MQ=255)

            cGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAg                                          <  1:1989614/69‑1 (MQ=255)

            cGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAg                                          <  1:2559068/69‑1 (MQ=255)

            cGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAg                                          <  1:800475/69‑1 (MQ=255)

            cGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAg                                          <  1:40401/69‑1 (MQ=255)

            cGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAg                                          <  1:418538/69‑1 (MQ=255)

            cGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAg                                          <  1:500674/69‑1 (MQ=255)

            cGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATa                                                                <  1:1060630/47‑1 (MQ=255)

            cGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATa                                                                <  1:3226997/47‑1 (MQ=255)

            cGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATa                                                                <  1:164216/47‑1 (MQ=255)

            cGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATa                                                                <  1:2260006/47‑1 (MQ=255)

            cGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATa                                                                <  1:1864704/47‑1 (MQ=255)

            cGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATa                                                                <  1:364562/47‑1 (MQ=255)

            cGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAg                                          <  1:3112930/69‑1 (MQ=255)

            cGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAg                                          <  1:3118034/69‑1 (MQ=255)

            cGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAg                                          <  1:342015/69‑1 (MQ=255)

            cGACCACCGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAg                                          <  1:756268/69‑1 (MQ=255)

             gACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAg                                          <  1:399174/68‑1 (MQ=255)

              aCCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAg                                          <  1:2123909/67‑1 (MQ=255)

               ccACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATa                                                                <  1:764266/44‑1 (MQ=255)

                    ggAGATGCGGGCGAGGAAGATACTCATCTGGATAGCGTAAATCACCCACCATGCCCATTAg                                          <  1:60159/61‑1 (MQ=255)

                    ggAGATGCGGGCGAGGAAGATACTCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAg                                          <  1:3212658/61‑1 (MQ=255)

                                 aGGAAGATACTCATCTGTATAGCGTAAATCACCCACCATGCCCAGTAg                                          <  1:395253/48‑1 (MQ=255)

                                   gAAGATACTCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAg                                          <  1:2307737/46‑1 (MQ=255)

                                         aCTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgccgcc             >  1:2696061/1‑69 (MQ=255)

                                         aCTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgccgcc             >  1:3026812/1‑69 (MQ=255)

                                         aCTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgccgcc             >  1:2659752/1‑69 (MQ=255)

                                         aCTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgccgcc             >  1:1966298/1‑69 (MQ=255)

                                         aCTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgccgcc             >  1:2568559/1‑69 (MQ=255)

                                         aCTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgccgcc             >  1:1440113/1‑69 (MQ=255)

                                         aCTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgccgcc             >  1:1166878/1‑69 (MQ=255)

                                         aCTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGACCAGCACTGCGGGaa                   >  1:3022598/1‑63 (MQ=255)

                                           tCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAGAACACGGTCCAg                              >  1:1376698/1‑50 (MQ=255)

                                           tCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAGAACACGGTCCAGc                             >  1:1741365/1‑51 (MQ=255)

                                           tCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGc                        >  1:759886/1‑56 (MQ=255)

                                           tCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGc                        >  1:1158022/1‑56 (MQ=255)

                                           tCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGc                        >  1:1559937/1‑56 (MQ=255)

                                           tCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGc                        >  1:939475/1‑56 (MQ=255)

                                           tCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGc                        >  1:599147/1‑56 (MQ=255)

                                           tCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGc                        >  1:156038/1‑56 (MQ=255)

                                           tCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGc                        >  1:800843/1‑56 (MQ=255)

                                           tCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGc                        >  1:483071/1‑56 (MQ=255)

                                           tCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGc                        >  1:1852055/1‑56 (MQ=255)

                                           tCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGc                        >  1:353094/1‑56 (MQ=255)

                                           tCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGc                        >  1:1036271/1‑56 (MQ=255)

                                           tCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGc                        >  1:3336283/1‑56 (MQ=255)

                                           tCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGc                        >  1:2656159/1‑56 (MQ=255)

                                           tCATCTGGATAGCGTAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGc                        >  1:3150491/1‑56 (MQ=255)

                                            cATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACgg                                   <  1:2786302/44‑1 (MQ=255)

                                            cATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACgg                                   <  1:1072988/44‑1 (MQ=255)

                                            cATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACgg                                   <  1:1282961/44‑1 (MQ=255)

                                            cATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACgg                                   <  1:2647970/44‑1 (MQ=255)

                                            cATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACgg                                   <  1:3186646/44‑1 (MQ=255)

                                            cATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACgg                                   <  1:2474009/44‑1 (MQ=255)

                                            cATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCttt          >  1:2165992/1‑69 (MQ=255)

                                            cATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCttt          >  1:2539375/1‑69 (MQ=255)

                                            cATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCttt          >  1:246799/1‑69 (MQ=255)

                                            cATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCttt          >  1:2052014/1‑69 (MQ=255)

                                            cATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCttt          >  1:2099938/1‑69 (MQ=255)

                                            cATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCttt          >  1:3325406/1‑69 (MQ=255)

                                            cATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCttt          >  1:3336910/1‑69 (MQ=255)

                                               cTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGc       <  1:2320978/69‑1 (MQ=255)

                                               cTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGc       <  1:1937139/69‑1 (MQ=255)

                                               cTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGc       <  1:2385726/69‑1 (MQ=255)

                                                tGGATAGCATAAATCACCCACCATGCCCAGTAGAACACgg                                   <  1:3005992/40‑1 (MQ=255)

                                                tGGATAGCATAAATCACCCACCATGCCCAGTAGAACACgg                                   <  1:1557100/40‑1 (MQ=255)

                                                tGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGc       <  1:2607916/68‑1 (MQ=255)

                                                tGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCg      >  1:2615541/1‑69 (MQ=255)

                                                tGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCg      >  1:2930698/1‑69 (MQ=255)

                                                tGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCg      >  1:209372/1‑69 (MQ=255)

                                                tGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCg      >  1:2858826/1‑69 (MQ=255)

                                                tGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCg      >  1:2855129/1‑69 (MQ=255)

                                                tGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCg      >  1:1576311/1‑69 (MQ=255)

                                                tGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTATAg        >  1:2896021/1‑67 (MQ=255)

                                                    tAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATgg  >  1:1694071/1‑69 (MQ=255)

                                                    tAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATgg  >  1:1772138/1‑69 (MQ=255)

TCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGG  >  minE/38573‑38693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`