| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 331,222 | A→G | 24.9% | V162V (GTT→GTC) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 331,222 | 0 | A | G | 24.9% | 102.8 / 31.3 | 88 | V162V (GTT→GTC) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base A (18/48); new base G (5/17); total (23/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
AGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTA > minE/331156‑331286 | agTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAAc < 1:1627926/69‑1 (MQ=255)agTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAAc < 1:1899591/69‑1 (MQ=255)agTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAAc < 1:2194958/69‑1 (MQ=255)agTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAAc < 1:1755538/69‑1 (MQ=255)agTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAAc < 1:2247438/69‑1 (MQ=255)agTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAAc < 1:1668880/69‑1 (MQ=255)agTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAAc < 1:1640162/69‑1 (MQ=255)agTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAAc < 1:2399584/69‑1 (MQ=255)agTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAAc < 1:384112/69‑1 (MQ=255)agTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAAc < 1:2782213/69‑1 (MQ=255)agTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAAc < 1:1417233/69‑1 (MQ=255)agTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAAc < 1:2950690/69‑1 (MQ=255)agTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAAc < 1:1260591/69‑1 (MQ=255)agTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAAc < 1:1128145/69‑1 (MQ=255)agTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAAc < 1:1106623/69‑1 (MQ=255) gTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAAcc > 1:2421848/1‑69 (MQ=255) gTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAAc < 1:561779/68‑1 (MQ=255) ggCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCTACCGCGGGTAGCAATACCAGCGTCAGGACCAt > 1:2881164/1‑69 (MQ=255) ggCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCTACCGCGGGTAGCAATACCAGCGTCAGGACCAt > 1:2107080/1‑69 (MQ=255) ggCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCTACCGCGGGTAGCAATACCAGCGTCAGGACCAt > 1:3187285/1‑69 (MQ=255) ggCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCTACCGCGGGTAGCAATACCAGCGTCAGGACCAt > 1:1674164/1‑69 (MQ=255) ggCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCTACCGCGGGTAGCAATACCAGCGTCAGGACCAt > 1:22846/1‑69 (MQ=255) aaaGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTa < 1:3138667/68‑1 (MQ=255) aTCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTc < 1:2912723/69‑1 (MQ=255) aTCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTc < 1:511011/69‑1 (MQ=255) aTCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTc < 1:107855/69‑1 (MQ=255) gTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATca > 1:3159453/1‑69 (MQ=255) gTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATca > 1:3172695/1‑69 (MQ=255) gTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATca > 1:1442489/1‑69 (MQ=255) tCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAAc < 1:1725189/44‑1 (MQ=255) ccATCATTCCTGCCACTGCGGGCAGCAACTCCAGCGTCAGAACCATTACCAGGTCTTCCACAATcaacc < 1:2822889/69‑1 (MQ=255) ccATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCTCAATcaacc > 1:1100292/1‑69 (MQ=255) ccATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATcaagc > 1:724166/1‑67 (MQ=255) ccATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATcaacc < 1:1952123/69‑1 (MQ=255) ccATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATcaacc > 1:275062/1‑69 (MQ=255) ccATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATcaacc > 1:170691/1‑69 (MQ=255) ccATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATcaacc < 1:2934221/69‑1 (MQ=255) ccATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATcaacc < 1:51721/69‑1 (MQ=255) ccATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATcaacc < 1:1296442/69‑1 (MQ=255) gCCACTGCGGTCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCg < 1:1970638/69‑1 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGGACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCg < 1:2313503/69‑1 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCaaccaacc < 1:3230003/62‑1 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCaaccaacc < 1:67764/62‑1 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCaaccaacc < 1:478540/62‑1 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCaaccaacc > 1:2078198/1‑62 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCaaccaacc < 1:2809508/62‑1 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCaaccaacc < 1:191972/62‑1 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCaaccaacc < 1:1089112/62‑1 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGc < 1:3259317/68‑1 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGc < 1:271006/68‑1 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCg < 1:1328558/69‑1 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCg < 1:1499726/69‑1 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCg < 1:2481332/69‑1 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCg < 1:2585690/69‑1 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCg < 1:256417/69‑1 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCg < 1:1794323/69‑1 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCg < 1:181793/69‑1 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCg < 1:1837688/69‑1 (MQ=255) cGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATgg > 1:2404113/1‑60 (MQ=255) cGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGcc > 1:1605932/1‑69 (MQ=255) cGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGcc > 1:730854/1‑69 (MQ=255) cGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGcc > 1:1631935/1‑69 (MQ=255) cGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGcc > 1:229411/1‑69 (MQ=255) cGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGcc > 1:225420/1‑69 (MQ=255) cGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGcc > 1:2967247/1‑69 (MQ=255) agcaACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCCACCGATGGCGATTTGCCCAc > 1:1745750/1‑67 (MQ=255) agcaACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACg > 1:1962801/1‑68 (MQ=255) gcaATACCAGCGTCAGGACCATCACCATGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCt < 1:2395847/69‑1 (MQ=255) gcaATACCAGCGTCAGGACCATCACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCt < 1:2222830/69‑1 (MQ=255) gcaATACCAGCGTCAGGACCATCACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCt < 1:926420/69‑1 (MQ=255) gcaATACCAGCGTCAGGACCATCACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCt < 1:865938/69‑1 (MQ=255) gcaATACCAGCGTCAGGACCATCACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCt < 1:1492920/69‑1 (MQ=255) gcaATACCAGCGTCAGGACCATCACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCt < 1:1588060/69‑1 (MQ=255) gcaATACCAGCGTCAGGACCATCACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCt < 1:1729718/69‑1 (MQ=255) gcaATACCAGCGTCAGGACCATCACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCt < 1:562040/69‑1 (MQ=255) gcaATACCAGCGTCAGGACCATCACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCt < 1:3143420/69‑1 (MQ=255) gcaATACCAGCGTCAGGACCATCACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCt < 1:2360230/69‑1 (MQ=255) gcaATACCAGCGTCAGGACCATCACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCt < 1:2361429/69‑1 (MQ=255) gcaATACCAGCGTCAGGACCATCACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCt < 1:2654218/69‑1 (MQ=255) gcaATACCAGCGTCAGGACCATCACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCt < 1:33472/69‑1 (MQ=255) gcaATACCAGCGTCAGGACCATCACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCt < 1:2909792/69‑1 (MQ=255) gcaATACCAGCGTCAGGACCATCACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCt < 1:3255524/69‑1 (MQ=255) gcaATACCAGCGTCAGGACCATCACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCt < 1:301900/69‑1 (MQ=255) caACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTg < 1:660252/69‑1 (MQ=255) caACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTg < 1:292556/69‑1 (MQ=255) tCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCaatta < 1:2122848/69‑1 (MQ=255) tCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCaatta < 1:580323/69‑1 (MQ=255) tCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCaatta < 1:3310646/69‑1 (MQ=255) | AGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTA > minE/331156‑331286 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |