| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 331,345 | A→G | 24.3% | G121G (GGT→GGC) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 331,345 | 0 | A | G | 24.3% | 93.5 / 26.2 | 78 | G121G (GGT→GGC) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base A (21/38); new base G (14/5); total (35/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
AATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAG > minE/331282‑331390 | aattaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAGCCg < 1:168475/67‑1 (MQ=255)aattaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAGCCg < 1:2786580/67‑1 (MQ=255)aattaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAGCCg < 1:2739748/67‑1 (MQ=255)aattaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAGCCg < 1:1775281/67‑1 (MQ=255)aattaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAGCCg < 1:415207/67‑1 (MQ=255)aattaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCg < 1:1444843/67‑1 (MQ=255)aattaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCg < 1:572240/67‑1 (MQ=255)aattaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCg < 1:475439/67‑1 (MQ=255)aattaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCg < 1:1224732/67‑1 (MQ=255)aattaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGaa > 1:2354273/1‑69 (MQ=255)aattaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGaa > 1:2124132/1‑69 (MQ=255)aattaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGaa > 1:1122334/1‑69 (MQ=255)aattaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGaa > 1:2698682/1‑69 (MQ=255)aattaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGaa > 1:2372465/1‑69 (MQ=255)aattaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGaa > 1:618343/1‑69 (MQ=255)aattaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGaa > 1:704360/1‑69 (MQ=255)aattaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGa > 1:32008/1‑68 (MQ=255) taattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGTAAAGACATAAACCGAacac < 1:779474/69‑1 (MQ=255) taattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAacac < 1:1680476/69‑1 (MQ=255) taattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAacac < 1:1837038/69‑1 (MQ=255) taattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAacac < 1:1902062/69‑1 (MQ=255) taattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAacac < 1:2769932/69‑1 (MQ=255) taattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAacac < 1:2910533/69‑1 (MQ=255) taattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAacac < 1:711175/69‑1 (MQ=255) ttcttcAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGt < 1:3381562/69‑1 (MQ=255) ttcttcAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGt < 1:2464096/69‑1 (MQ=255) ttcttcAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGt < 1:3046752/69‑1 (MQ=255) ttcttcAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGt < 1:3299217/69‑1 (MQ=255) ttcttcAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGt < 1:3327394/69‑1 (MQ=255) ttcttcAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGt < 1:958634/69‑1 (MQ=255) ttcttcAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGt < 1:418646/69‑1 (MQ=255) ttcttcAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGt < 1:577782/69‑1 (MQ=255) ttcttcAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGt < 1:1023841/69‑1 (MQ=255) ttcttcAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGt < 1:1239766/69‑1 (MQ=255) ttcttcAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGt < 1:1697909/69‑1 (MQ=255) ttcttcAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGt < 1:2177363/69‑1 (MQ=255) ttcttcAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGt < 1:2293069/69‑1 (MQ=255) ttcttcAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGt < 1:2026523/69‑1 (MQ=255) tcttcAAGTGCGCGCAGTAACACCACGGTACTGGCTGTGGAAAGACATAAACCGAACACGATACCGGt < 1:2818637/68‑1 (MQ=255) aaCACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATAcc > 1:183475/1‑47 (MQ=255) aaCACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAccagcccagc > 1:3330334/1‑69 (MQ=255) aaCACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAccagcccagc < 1:1109449/69‑1 (MQ=255) aaCACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAccagcccagc > 1:1406166/1‑69 (MQ=255) aaCACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAccagcccagc > 1:1635324/1‑69 (MQ=255) aaCACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAccagcccagc > 1:1733624/1‑69 (MQ=255) aaCACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAccagcccagc < 1:2816184/69‑1 (MQ=255) aaCACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAccagcccagc < 1:2060180/69‑1 (MQ=255) aaCACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAccagcccagc > 1:2669705/1‑69 (MQ=255) aaCACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAccagcccagc > 1:2060474/1‑69 (MQ=255) aaCACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAccagcccagc > 1:2183222/1‑69 (MQ=255) aaCACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAccagcccagc > 1:267383/1‑69 (MQ=255) aaCACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAccagcccag > 1:3276963/1‑68 (MQ=255) acacCACGGTACTGGCGGTGGATAGACATAAGCCGAac > 1:3004519/1‑38 (MQ=38) acacCACGGTACTGGCGGTGGAAAGACATAAGCCGaa > 1:43924/1‑37 (MQ=255) acacCACGGTACTGGCGGTGGAAAGACATAAGCCGAac > 1:2379424/1‑38 (MQ=255) acacCACGGTACTGGCGGTGGAAAGACATAAGCCGAac > 1:2057620/1‑38 (MQ=255) acacCACGGTACTGGCGGTGGAAAGACATAAGCCGAac > 1:1327276/1‑38 (MQ=255) acacCACGGTACTGGCGGTGGAAAGACATAAGCCGAac > 1:2517660/1‑38 (MQ=255) acacCACGGTACTGGCGGTGGAAAGACATAAGCCGAac > 1:54902/1‑38 (MQ=255) acacCACGGTACTGGCGGTGGAAAGACATAAGCCGAac > 1:1655486/1‑38 (MQ=255) acacCACGGTACTGGCGGTGGAAAGACATAAGCCGAac > 1:443647/1‑38 (MQ=255) acacCACGGTACTGGCGGTGGAAAGACATAAGCCGAac > 1:360536/1‑38 (MQ=255) acacCACGGTACTGGCGGTGGAAAGACATAAGCCGAac > 1:3248923/1‑38 (MQ=255) acacCACGGTACTGGCGGTGGAAAGACATAAGCCGAac > 1:3322975/1‑38 (MQ=255) acacCACGGTACTGGCGGTGGAAAGACATAAGCCGAac > 1:3345823/1‑38 (MQ=255) acacCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCa > 1:2255450/1‑69 (MQ=255) acacCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCa > 1:1138221/1‑69 (MQ=255) acacCAAGGTACTGGCGGTGGAAAGACATAAGCCGAac > 1:3358934/1‑38 (MQ=37) gTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCagag < 1:347963/69‑1 (MQ=255) gTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCagag < 1:1617793/69‑1 (MQ=255) gTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCagag < 1:1709435/69‑1 (MQ=255) gTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCagag < 1:777025/69‑1 (MQ=255) gTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCagag < 1:956472/69‑1 (MQ=255) gTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCagag < 1:957443/69‑1 (MQ=255) gTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCagag < 1:3113407/69‑1 (MQ=255) aCTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCagag < 1:1818482/67‑1 (MQ=255) ggCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAAc > 1:1992032/1‑43 (MQ=255) aaaGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCagag < 1:93973/56‑1 (MQ=255) | AATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAG > minE/331282‑331390 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |