| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 333,415 | G→A | 30.7% | A11A (GCG→GCA) | ushA → | bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 333,415 | 0 | G | A | 30.7% | 59.6 / 29.5 | 65 | A11A (GCG→GCA) | ushA | bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase |
| Reads supporting (aligned to +/- strand): ref base G (12/33); new base A (6/14); total (18/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGCTCTGGCGTATGAGCAGGATAAAACC > minE/333348‑333475 | tttCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAgcgc > 1:897202/1‑69 (MQ=255)tttCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAgcgc > 1:2118365/1‑69 (MQ=255)tttCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAgcgc > 1:2165426/1‑69 (MQ=255)tttCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAgcgc > 1:455519/1‑69 (MQ=255)tttCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAgcgc > 1:57027/1‑69 (MQ=255)tttCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGAAGCGGGGCGTGGCGTTAgcgc > 1:3217576/1‑69 (MQ=255)tttCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATCGCAGCGGGGCGTGGCGTTAgcgc > 1:136183/1‑69 (MQ=255)tttCAACACGTTGAAATCAGGTCACGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAgcgc > 1:2104150/1‑69 (MQ=255) ttGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCACTGTTAACcac > 1:643400/1‑69 (MQ=255) ttGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCACTGTTAACcac > 1:1285374/1‑69 (MQ=255) ttGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCACTGTTAACcac > 1:1056155/1‑69 (MQ=255) ttGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCACTGTTAACcac > 1:1000765/1‑69 (MQ=255) ttGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCACTGTTAACcac > 1:2550298/1‑69 (MQ=255) ttGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCACTGATAACcac > 1:246615/1‑69 (MQ=255) aaTCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACAtt < 1:559597/68‑1 (MQ=255) aTCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTa < 1:1262903/69‑1 (MQ=255) aTCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTa < 1:698943/69‑1 (MQ=255) aTCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTa < 1:3224734/69‑1 (MQ=255) aTCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTa < 1:1167183/69‑1 (MQ=255) aTCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTa < 1:2569753/69‑1 (MQ=255) gtcaggGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTg > 1:1077119/1‑69 (MQ=255) gtcaggGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTg > 1:2835964/1‑69 (MQ=255) gagaAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGa < 1:3277696/69‑1 (MQ=255) gagaAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGa < 1:721817/69‑1 (MQ=255) gagaAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGa < 1:2441227/69‑1 (MQ=255) gagaAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGa < 1:1015862/69‑1 (MQ=255) agaAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGaa < 1:1974373/69‑1 (MQ=255) agaAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGaa < 1:2077448/69‑1 (MQ=255) agaAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGaa < 1:1090833/69‑1 (MQ=255) agaAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGaa < 1:661313/69‑1 (MQ=255) agaAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGaa < 1:2130401/69‑1 (MQ=255) agaAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGaa < 1:1419013/69‑1 (MQ=255) agaAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGaa < 1:1439044/69‑1 (MQ=255) agaAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGaa < 1:1453953/69‑1 (MQ=255) agaAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGaa < 1:2250232/69‑1 (MQ=255) agaAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGaa < 1:2422132/69‑1 (MQ=255) gaAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGaa < 1:2868988/68‑1 (MQ=255) tATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGc < 1:418288/69‑1 (MQ=255) tATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGc < 1:1280147/69‑1 (MQ=255) tGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGctc > 1:1023183/1‑69 (MQ=255) tGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGctc > 1:2624033/1‑69 (MQ=255) tGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGctc > 1:1398251/1‑69 (MQ=255) tGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGctc > 1:1206759/1‑69 (MQ=255) tGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGctc > 1:1431407/1‑69 (MQ=255) tGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGctc > 1:1147216/1‑69 (MQ=255) ttattGCAGCTGGGCGTGGCGTTAGCACTGTTAACcac < 1:3061377/38‑1 (MQ=38) ttattGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGCTCTTGCg < 1:3387408/69‑1 (MQ=255) ttattGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGCTCTGGCg < 1:1332288/69‑1 (MQ=255) ttattGCAGCGGGGCGTGGCGTTAGCACTGTTAACcac < 1:1731591/38‑1 (MQ=255) ttattGCAGCGGGGCGTGGCGTTAGCACTGTTAACcac < 1:342003/38‑1 (MQ=255) ttattGCAGCGGGGCGTGGCGTTAGCACTGTTAACcac < 1:3363480/38‑1 (MQ=255) ttattGCAGCGGGGCGTGGCGTTAGCACTGTTAACcac < 1:618646/38‑1 (MQ=255) ttattGCAGCGGGGCGTGGCGTTAGCACTGTTAACcac < 1:2763923/38‑1 (MQ=255) ttattGCAGCGGGGCGTGGCGTTAGCACTGTTAACcac < 1:66676/38‑1 (MQ=255) ttattGCAGCGGGGCGTGGCGTTAGCACTGTTAACcac < 1:262874/38‑1 (MQ=255) ttattGCAGCGGGGCGTGGCGTTAGCACTGTTAACcac < 1:2216023/38‑1 (MQ=255) ttattGCAGCGGGGCGTGGCGTTAGCACTGTTAACcac < 1:2221558/38‑1 (MQ=255) ttattGCAGCGGGGCGTGGCGTTAGCACTGTTAACcac < 1:1773385/38‑1 (MQ=255) ttattGCAGCGGGGCGTGGCGTTAGCACTGTTAACcac < 1:1477112/38‑1 (MQ=255) ttattGCAGCGGGGCGTGGCGTTAGCACTGTTAACcac < 1:2461442/38‑1 (MQ=255) ttattGCAGCGGGGCGTGGCGTTAGCACTGTCAACcac < 1:435289/38‑1 (MQ=38) gcgtTAGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGCTCTGGCGTATGAGCAGGATAAAAcc > 1:1952211/1‑69 (MQ=255) gcgtTAGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGCTCTGGCGTATGAGCAGGATAAAAcc > 1:3424761/1‑69 (MQ=255) gcgtTAGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGCTCTGGCGTATGAGCAGGATAAAAcc > 1:303416/1‑69 (MQ=255) gcgtTAGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGCTCTGGCGTATGAGCAGGATAAAAcc > 1:2920059/1‑69 (MQ=255) aGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGCTCTGGCg < 1:1689585/46‑1 (MQ=255) aGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGCTCTGGCg < 1:3205715/46‑1 (MQ=255) aGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGCTCTGGCg < 1:3137958/46‑1 (MQ=255) aGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGCTCTGGCg < 1:1181918/46‑1 (MQ=255) aGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGCTCTGGCg < 1:1879804/46‑1 (MQ=255) aGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGCTCTGGCg < 1:2657338/46‑1 (MQ=255) aGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGCTCTGGCg < 1:2501422/46‑1 (MQ=255) aGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGCTCTGGCg < 1:1663265/46‑1 (MQ=255) gcTGTTAACCACATTTACACTGGCGAGTGAAACTGc < 1:2525461/36‑1 (MQ=255) | TTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTGCAGCGGGGCGTGGCGTTAGCGCTGTTAACCACATTTACACTGGCGAGTGAAACTGCTCTGGCGTATGAGCAGGATAAAACC > minE/333348‑333475 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |