| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 333,931 | C→T | 42.8% | Y183Y (TAC→TAT) | ushA → | bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 333,931 | 0 | C | T | 42.8% | 6.8 / 23.1 | 35 | Y183Y (TAC→TAT) | ushA | bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase |
| Reads supporting (aligned to +/- strand): ref base C (6/14); new base T (7/8); total (13/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
CAGGATCTGAAAATTGCCGTTATTGGGCTGACAACCGATGACACAGCAAAAATTGGTAACCCGGAATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGC > minE/333863‑333992 | cAGGATCTGAAAATTGCCGTTATTGGGCTGACAACCGATGACACAGCAAAAATTGGTAACCCGGAATAc < 1:2547042/69‑1 (MQ=255)cAGGATCTGAAAATTGCCGTTATTGGGCTGACAACCGATGACACAGCAAAAATTGGTAACCCGGAATAc < 1:1483399/69‑1 (MQ=255) aaaaTTGCCGTTATTGGCCTGACGACGGATGACACAGCCAAAATTGGTAATCCGGAATATTTCACTGat > 1:2729891/1‑69 (MQ=255) aaaaTTGCCGTTATTGGCCTGACGACGGATGACACAGCCAAAATTGGTAATCCGGAATATTTCACTGat > 1:2558430/1‑69 (MQ=255) aaaaTTGCCGTTATTGGCCTGACGACGGATGACACAGCCAAAATTGGTAATCCGGAATATTTCACTGat > 1:480439/1‑69 (MQ=255) aaaaTTGCCGTTATTGGCCTGACGACGGATGACACAGCCAAAATTGGTAATCCGGAATATTTCACTGat > 1:1595216/1‑69 (MQ=255) aaaaTTGCCGTTATTGGCCTGACGACGGATGACACAGCCAAAATTGGTAATCCGGAATATTTCACTGat > 1:1758389/1‑69 (MQ=255) aaaaTTGCCGTTATTGGCCTGACGACGGATGACACAGCCAAAATTGGTAATCCGGAATATTTCACTGat > 1:1762190/1‑69 (MQ=255) aaaaTTGCCGTTATTGGCCTGACGACGGATGACACAGCCAAAATTGGTAATCCGGAATATTTCACTGa > 1:1938558/1‑68 (MQ=255) aaTTGCCGTTATTGGGCTGACAACCGATGACACAGCAAAAATTGGTAACCCGGAATACTTCACt < 1:1309223/64‑1 (MQ=255) aTTGGGCTGACAACCGATGACACAGCAAAAATTGGTAACCCGGAATACTTCACTGATATCGAATTTc > 1:1188132/1‑67 (MQ=255) gggCTGACAACCGATGACACAGCAAAAATTGGTAACCCGGAATACTTCACTGATATCGAATTTCGTaa > 1:609918/1‑68 (MQ=255) gggCTGACAACCGATGACACAGCAAAAATTGGTAACCCGGAATACTTCACTGATATCGAATTTCGTAAg > 1:1195916/1‑69 (MQ=255) gggCTGACAACCGATGACACAGCAAAAATTGGTAACCCGGAATACTTCACTGATATCGAATTTCGTAAg > 1:1776793/1‑69 (MQ=255) cacaGCAAAAATTGGTAACCCGGAATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATgaagcga < 1:2792971/68‑1 (MQ=255) aGCAAAAATTGGTAACCCGGAATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCt < 1:43925/69‑1 (MQ=255) aGCAAAAATTGGTAACCCGGAATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCt < 1:3066819/69‑1 (MQ=255) aGCAAAAATTGGTAACCCGGAATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCt < 1:1212938/69‑1 (MQ=255) aGCAAAAATTGGTAACCCGGAATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCt < 1:1075727/69‑1 (MQ=255) gCAAAAATTGGTAACCCGGAATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCt > 1:2540587/1‑68 (MQ=255) gCAAAAATTGGTAACCCGGAATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTg > 1:1940635/1‑69 (MQ=255) aaaaaTTGGTAACCCGGAATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGt < 1:3123465/69‑1 (MQ=255) aaaaaTTGGTAACCCGGAATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGt < 1:121868/69‑1 (MQ=255) aaaaaTTGGTAACCCGGAATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGt < 1:2119162/69‑1 (MQ=255) aaaaaTTGGTAACCCGGAATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGt < 1:2481927/69‑1 (MQ=255) aaaaaTTGGTAACCCGGAATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGt < 1:1126872/69‑1 (MQ=255) aaaaaTTGGTAACCCGGAATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGt < 1:3038500/69‑1 (MQ=255) aTTGGTAATCCGGAATATTTCACTGATATCGAATTTCGTAAGCCCGCTGATGAAGCGAAGCTGGTGAtt < 1:923368/69‑1 (MQ=255) aTTGGTAATCCGGAATATTTCACTGATATCGAATTTCGTAAGCCCGCTGATGAAGCGAAGCTGGTGAtt < 1:2991566/69‑1 (MQ=255) aTTGGTAATCCGGAATATTTCACTGATATCGAATTTCGTAAGCCCGCTGATGAAGCGAAGCTGGTGAtt < 1:2586594/69‑1 (MQ=255) aTTGGTAATCCGGAATATTTCACTGATATCGAATTTCGTAAGCCCGCTGATGAAGCGAAGCTGGTGAtt < 1:195712/69‑1 (MQ=255) aTTGGTAATCCGGAATATTTCACTGATATCGAATTTCGTAAGCCCGCTGATGAAGCGAAGCTGGTGAtt < 1:1865253/69‑1 (MQ=255) aTTGGTAATCCGGAATATTTCACTGATATCGAATTTCGTAAGCCCGCTGATGAAGCGAAGCTGGTGAtt < 1:1801350/69‑1 (MQ=255) aTTGGTAATCCGGAATATTTCACTGATATCGAATTTCGTAAGCCCGCTGATGAAGCGAAGCTGGTGAtt < 1:1153499/69‑1 (MQ=255) aTTGGTAATCCGGAATATTTCACTGATATCGAATTTCGTAAGCCCGCTGATGAAGCGAAGCTGGTGAtt < 1:1144383/69‑1 (MQ=255) cccGGAATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGt < 1:3232371/57‑1 (MQ=255) cGGAATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGc < 1:1090385/69‑1 (MQ=255) | CAGGATCTGAAAATTGCCGTTATTGGGCTGACAACCGATGACACAGCAAAAATTGGTAACCCGGAATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGC > minE/333863‑333992 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |