| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 334,651 | G→A | 39.7% | V423V (GTG→GTA) | ushA → | bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 334,651 | 0 | G | A | 39.7% | 36.6 / 51.8 | 78 | V423V (GTG→GTA) | ushA | bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase |
| Reads supporting (aligned to +/- strand): ref base G (10/37); new base A (10/21); total (20/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01 | |||||||||||
AGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGTGATT > minE/334595‑334714 | aGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCa > 1:181796/1‑68 (MQ=255)aGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCa > 1:2510726/1‑68 (MQ=255)aGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCa > 1:2216432/1‑68 (MQ=255)aGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCa > 1:2295014/1‑68 (MQ=255) cGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGc < 1:2591873/68‑1 (MQ=255) cGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGc < 1:30384/68‑1 (MQ=255) cGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGc < 1:1443131/68‑1 (MQ=255) cGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGc < 1:1287825/68‑1 (MQ=255) cGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGc < 1:1267618/68‑1 (MQ=255) ggAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGc < 1:1590248/67‑1 (MQ=255) ggAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGc < 1:2758037/69‑1 (MQ=255) ggAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGc < 1:1828273/69‑1 (MQ=255) aaTTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTTAAAGTGCAGCCATTCGGCaa < 1:681927/69‑1 (MQ=255) aaTTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCaa < 1:2802829/69‑1 (MQ=255) aaTTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCaa < 1:3293920/69‑1 (MQ=255) aaTTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCaa < 1:1858327/69‑1 (MQ=255) aaTTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCaa < 1:2572595/69‑1 (MQ=255) aaTTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCaa < 1:1704431/69‑1 (MQ=255) aTTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCaa < 1:1977927/68‑1 (MQ=255) aTTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCaa < 1:2914598/68‑1 (MQ=255) aTTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCaa < 1:3248331/68‑1 (MQ=255) tCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATgtggtggtg < 1:2130628/69‑1 (MQ=255) tCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATgtggtggtg < 1:2020835/69‑1 (MQ=255) gATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGAcc > 1:1662676/1‑69 (MQ=255) gATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGAcc > 1:193617/1‑69 (MQ=255) tatCAGCTATAAAAACGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGAcc > 1:61541/1‑67 (MQ=255) tatCAGCTATAAAAACGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCg > 1:912411/1‑68 (MQ=255) tatCAGCTATAAAAACGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCg > 1:770518/1‑68 (MQ=255) tatCAGCTATAAAAACGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCg > 1:2819029/1‑68 (MQ=255) tatCAGCTATAAAAACGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCg > 1:390065/1‑68 (MQ=255) tatCAGCTATAAAAACGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCg > 1:2832457/1‑68 (MQ=255) tatCAGCTATAAAAACGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCg > 1:3264027/1‑68 (MQ=255) tatCAGCTATAAAAACGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCg > 1:1586913/1‑68 (MQ=255) tatCAGCTATAAAAACGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCg > 1:3042564/1‑68 (MQ=255) tatCAGCTATAAAAACGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCg > 1:1683752/1‑68 (MQ=255) atCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCgg > 1:2689306/1‑68 (MQ=255) atCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCgg > 1:3276652/1‑68 (MQ=255) tCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGTCAATgtggtggtg < 1:362391/50‑1 (MQ=255) tCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATgtggtggtg < 1:832964/50‑1 (MQ=255) tCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATgtggtggtg < 1:2854532/50‑1 (MQ=255) tCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATgtggtggtg < 1:75283/50‑1 (MQ=255) tCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATgtggtggtg < 1:396786/50‑1 (MQ=255) tCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATgtggtggtg < 1:3393655/50‑1 (MQ=255) tCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATgtggtggtg < 1:2404645/50‑1 (MQ=255) tCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATgtggtggtg < 1:1158215/50‑1 (MQ=255) tCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATgtggtggtg < 1:145158/50‑1 (MQ=255) tCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATgtggtggtg < 1:2389302/50‑1 (MQ=255) tCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATgtggtggtg < 1:222670/50‑1 (MQ=255) tCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATgtggtggtg < 1:1475266/50‑1 (MQ=255) tCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATgtggtggtg < 1:1845486/50‑1 (MQ=255) tCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATgtggtggtg < 1:1789823/50‑1 (MQ=255) cAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATgtggtggtg < 1:1864515/49‑1 (MQ=255) aaaaCGTGCTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTaa > 1:111889/1‑61 (MQ=255) aaaaCGTGCTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtga > 1:1186152/1‑69 (MQ=255) aaaaCGTGCTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtg < 1:1486703/68‑1 (MQ=255) aaaaCGTGCTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGt < 1:2038288/67‑1 (MQ=255) aaaaCGTGCTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGt < 1:146880/67‑1 (MQ=255) aaCGTACTGAAAGTGCAGCCATTCGTCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:1493579/69‑1 (MQ=255) aaCGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:1692205/69‑1 (MQ=255) aaCGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:856090/69‑1 (MQ=255) aaCGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:2698010/69‑1 (MQ=255) aaCGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:1169241/69‑1 (MQ=255) aaCGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:2740890/69‑1 (MQ=255) aaCGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:1263650/69‑1 (MQ=255) aaCGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:638383/69‑1 (MQ=255) aaCGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:134461/69‑1 (MQ=255) aaCGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:511960/69‑1 (MQ=255) aaCGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:474601/69‑1 (MQ=255) aaCGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:1101260/69‑1 (MQ=255) aaCGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:1459883/69‑1 (MQ=255) aaCGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:2034920/69‑1 (MQ=255) aaCGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:2930692/69‑1 (MQ=255) aaCGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:2948250/69‑1 (MQ=255) aaCGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:3286693/69‑1 (MQ=255) aaCGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:2641264/69‑1 (MQ=255) aaCGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:3265895/69‑1 (MQ=255) aaCGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:1825870/69‑1 (MQ=255) aCGTACTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGtgatt < 1:2576029/68‑1 (MQ=255) | AGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGTGATT > minE/334595‑334714 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |