| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 335,821 | T→G | 59.7% | R243R (CGA→CGC) | ybaP ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 335,821 | 0 | T | G | 59.7% | 40.9 / 54.8 | 67 | R243R (CGA→CGC) | ybaP | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (3/24); new base G (16/24); total (19/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.28e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-01 | |||||||||||
CATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCAGCACATCG > minE/335764‑335887 | cATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCcggcgg < 1:627583/69‑1 (MQ=255)cATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCcggcgg < 1:425785/69‑1 (MQ=255)cATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCcggcgg < 1:196225/69‑1 (MQ=255)cATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCcggcgg < 1:1990377/69‑1 (MQ=255)cATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCcggcgg < 1:2283003/69‑1 (MQ=255)cATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCcggcgg < 1:2351681/69‑1 (MQ=255)cATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCcggcgg < 1:3143533/69‑1 (MQ=255)cATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCcggcgg < 1:124187/69‑1 (MQ=255)cATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCcggcgg < 1:273163/69‑1 (MQ=255)cATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCcggcgg < 1:3087518/69‑1 (MQ=255)cATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCcggcgg < 1:2855480/69‑1 (MQ=255)cATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCcggcgg < 1:3083545/69‑1 (MQ=255) tGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATg < 1:336186/69‑1 (MQ=255) tGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATg < 1:2858878/69‑1 (MQ=255) aGGTGTAGTGCACCGACCGCGACCACATATCGCCCcggcgg < 1:451596/41‑1 (MQ=255) cACCGAGCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCt > 1:440796/1‑69 (MQ=255) cACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGcc > 1:1754542/1‑54 (MQ=255) cACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCt > 1:1630654/1‑69 (MQ=255) cACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCt < 1:3410405/69‑1 (MQ=255) cACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCt < 1:1537217/69‑1 (MQ=255) cACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCt < 1:1522695/69‑1 (MQ=255) ccgactgCGACCACATAGCGCCCCGGCGGCATGGCGCGTAAtt < 1:3186393/43‑1 (MQ=38) ccgactgCGACCACATAGCGCCCCGGCGGCATGGCGCGTAAtt < 1:2918948/43‑1 (MQ=38) ccgactgCGACCACATAGCGCCCCGGCGGCATGGCGCGTAAtt < 1:1267840/43‑1 (MQ=38) ccgactgCGACCACATAGCGCCCCGGCGGCATGGCGCGTAAtt < 1:1075279/43‑1 (MQ=38) ccgactgCGACCACATAGCGCCCCGGCGGCATGGCGCGTAAtt < 1:2545886/43‑1 (MQ=38) ccgactgCGACCACATAGCGCCCCGGCGGCATGGCGCGTAAtt < 1:49848/43‑1 (MQ=38) ccgactgCGACCACATAGCGCCCCGGCGGCATGGCGCGTAAtt < 1:1606998/43‑1 (MQ=38) ccgactgCGACCACATAGCGCCCCGGCGGCATGGCGCGTAAtt < 1:1498672/43‑1 (MQ=38) ccgactgCGACCACATAGCGCCCCGGCGGCATGGCGCGTAAtt < 1:1999745/43‑1 (MQ=38) ccgactgCGACCACATAGCGCCCCGGCGGCATGGCGCGTAAtt < 1:3405184/43‑1 (MQ=38) ccgactgCGACCACATAGCGCCCCGGCGGCATGGCGCGTAAtt < 1:411711/43‑1 (MQ=38) ccgactgCGACCACATAGCGCCCCGGCGGCATGGCGCGTAAtt < 1:418484/43‑1 (MQ=38) accgCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc < 1:1471915/69‑1 (MQ=255) accgCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc < 1:3158607/69‑1 (MQ=255) accgCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc < 1:1230627/69‑1 (MQ=255) accgCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc < 1:928939/69‑1 (MQ=255) accgCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc < 1:1538050/69‑1 (MQ=255) accgCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc < 1:929414/69‑1 (MQ=255) tgcgACCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc > 1:2568811/2‑67 (MQ=255) tgcgACCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc > 1:823533/2‑67 (MQ=255) tgcgACCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc > 1:846401/2‑67 (MQ=255) tgcgACCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc > 1:1774170/2‑67 (MQ=255) tgcgACCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc > 1:1090771/2‑67 (MQ=255) tgcgACCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc > 1:110815/2‑67 (MQ=255) tgcgACCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc > 1:13407/2‑67 (MQ=255) tgcgACCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc > 1:1511213/2‑67 (MQ=255) tgcgACCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc > 1:1528630/2‑67 (MQ=255) tgcgACCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc > 1:1650165/2‑67 (MQ=255) tgcgACCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc > 1:1769842/2‑67 (MQ=255) tgcgACCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc > 1:2974093/2‑67 (MQ=255) tgcgACCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc > 1:2932660/2‑67 (MQ=255) tgcgACCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc > 1:2607545/2‑67 (MQ=255) tgcgACCACATAGCGCCACGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGc > 1:619087/2‑67 (MQ=255) tgcgACCACATAGCG‑CCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATc > 1:1630769/2‑69 (MQ=255) gACCACATAGCGCCCCGGCGGCATGGCGCGTAAtt < 1:2103158/35‑1 (MQ=255) aCCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCa < 1:3269013/67‑1 (MQ=255) aCCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCa < 1:1159494/67‑1 (MQ=255) aCCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCa < 1:837367/67‑1 (MQ=255) aCCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCa < 1:2777449/67‑1 (MQ=255) aCCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCa < 1:1386514/67‑1 (MQ=255) aCCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCa < 1:3108189/67‑1 (MQ=255) aCCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCa < 1:3153274/67‑1 (MQ=255) aCCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCa < 1:2375275/67‑1 (MQ=255) aCCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCa < 1:2251362/67‑1 (MQ=255) aCCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCa < 1:1945042/67‑1 (MQ=255) aCCACATAGCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCa < 1:3186593/67‑1 (MQ=255) tCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCAGCACATCg > 1:1163228/1‑67 (MQ=255) tCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCAGCACATCg > 1:3022888/1‑67 (MQ=255) | CATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCAGCACATCG > minE/335764‑335887 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |