| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 338,292 | A→G | 100% | T519T (ACT→ACC) | copA ← | copper transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 338,292 | 0 | A | G | 85.2% | 30.6 / ‑1.5 | 20 | T519T (ACT→ACC) | copA | copper transporter |
| Reads supporting (aligned to +/- strand): ref base A (3/0); new base G (0/17); total (3/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCC > minE/338265‑338358 | cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGGGCGTTGCAGCGCGTCGGCGTcc < 1:1805894/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:1312627/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:847268/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:641406/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:3393140/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:3390427/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:3164335/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:3084357/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:3017619/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:2882707/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:2241754/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:2218393/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:2157303/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:1828141/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:1791469/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:1605583/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:1180987/67‑1 (MQ=255) aCAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTcc > 1:1782407/1‑42 (MQ=255) aCAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAgccgcc > 1:2361376/1‑69 (MQ=255) aCAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAgccgcc > 1:2566846/1‑69 (MQ=255) | CAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCC > minE/338265‑338358 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |