| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 338,325 | A→G | 100% | A508A (GCT→GCC) | copA ← | copper transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 338,325 | 0 | A | G | 75.2% | 25.4 / 4.2 | 24 | A508A (GCT→GCC) | copA | copper transporter |
| Reads supporting (aligned to +/- strand): ref base A (3/3); new base G (0/18); total (3/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.88e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAA > minE/338265‑338377 | cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGGGCGTTGCAGCGCGTCGGCGTcc < 1:1805894/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:1180987/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:847268/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:641406/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:3393140/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:3390427/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:3164335/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:3084357/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:3017619/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:2882707/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:2241754/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:2218393/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:2157303/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:1828141/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:1791469/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:1605583/67‑1 (MQ=255)cagCGTCCCGGTTTTATCGAACACTACGGTGTCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:1312627/67‑1 (MQ=255) aCAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTcc > 1:1782407/1‑42 (MQ=255) aCAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAgccgcc > 1:2361376/1‑69 (MQ=255) aCAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAgccgcc > 1:2566846/1‑69 (MQ=255) gtgtCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGccgcccg < 1:633689/68‑1 (MQ=255) tCGAGCGTACTGGCGCGTTGCAGCGCGTCGGCGTcc < 1:3111119/36‑1 (MQ=25) gcgTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAaataa < 1:1567511/68‑1 (MQ=255) gcgTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAaataa < 1:1086870/68‑1 (MQ=255) | CAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAA > minE/338265‑338377 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |