| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 346,941 | C→G | 47.4% | W224S (TGG→TCG) | ybbO ← | predicted oxidoreductase with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 346,941 | 0 | C | G | 47.4% | 1.9 / 66.3 | 57 | W224S (TGG→TCG) | ybbO | predicted oxidoreductase with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base C (12/18); new base G (7/20); total (19/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.20e-01 | |||||||||||
TTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATC > minE/346903‑347003 | ttCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGt < 1:1606600/54‑1 (MQ=255)ttCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGt < 1:1600141/54‑1 (MQ=255)ttCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAgcgg > 1:1318239/1‑69 (MQ=255)ttCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAgcgg > 1:409230/1‑69 (MQ=255)ttCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAgcgg > 1:413252/1‑69 (MQ=255)ttCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAgcgg > 1:100794/1‑69 (MQ=255)ttCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAgcgg > 1:2234890/1‑69 (MQ=255) cTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGggcggc < 1:616847/69‑1 (MQ=255) cTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGggcggc < 1:3076560/69‑1 (MQ=255) cTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGggcggc < 1:1247461/69‑1 (MQ=255) cTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGggcggc < 1:691241/69‑1 (MQ=255) cTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGggcggc < 1:1154426/69‑1 (MQ=255) cTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGggcggc < 1:2118639/69‑1 (MQ=255) cTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGggcggc < 1:2962193/69‑1 (MQ=255) cTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGggcggc < 1:2323055/69‑1 (MQ=255) cTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGggcggc < 1:1966882/69‑1 (MQ=255) cTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGggcggc < 1:1852809/69‑1 (MQ=255) cTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGggcggc < 1:2493101/69‑1 (MQ=255) cTTCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTT‑CGGTCCCAACGTAAAGCGGgcggcg < 1:530892/69‑1 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGTTCCCAACGTAAAGCGGgcggcg < 1:1838553/69‑1 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg < 1:2609368/69‑1 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg < 1:2869927/69‑1 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg < 1:2969644/69‑1 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg > 1:2473777/1‑69 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg > 1:3041730/1‑69 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg > 1:44408/1‑69 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg < 1:476639/69‑1 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg < 1:604670/69‑1 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg > 1:672423/1‑69 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg < 1:792759/69‑1 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg < 1:965883/69‑1 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg < 1:187028/69‑1 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg < 1:1115438/69‑1 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg < 1:1177022/69‑1 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg < 1:1205063/69‑1 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg < 1:1244777/69‑1 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg > 1:1613798/1‑69 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg < 1:1733536/69‑1 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg < 1:1926541/69‑1 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg > 1:214049/1‑69 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg < 1:243324/69‑1 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg < 1:2257716/69‑1 (MQ=255) ttCTCGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg > 1:2142402/1‑69 (MQ=255) tctcGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg < 1:3262494/68‑1 (MQ=255) tctcGCTAATAAAAGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGgcggcg < 1:652614/68‑1 (MQ=255) cGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGc > 1:2814209/1‑69 (MQ=255) cGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGc > 1:2340329/1‑69 (MQ=255) cGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGc > 1:3066401/1‑69 (MQ=255) aaaaGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGAtt > 1:515492/1‑69 (MQ=255) aaaaGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGAtt > 1:1801472/1‑69 (MQ=255) aaaaGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGAtt > 1:2878208/1‑69 (MQ=255) aaaaGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGAtt > 1:1192052/1‑69 (MQ=255) tACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGttt < 1:2536551/69‑1 (MQ=255) tACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGttt < 1:780959/69‑1 (MQ=255) tACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGttt < 1:3162431/69‑1 (MQ=255) aCTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTTGttt < 1:3328116/68‑1 (MQ=255) ttGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATc < 1:1133827/68‑1 (MQ=255) | TTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATC > minE/346903‑347003 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |