Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 348,014 A→T 47.4% A104A (GCA→GCT)  gcl → glyoxylate carboligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE348,0140AT47.4% 1.8 / 74.3 67A104A (GCA→GCT) gclglyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base A (14/21);  new base T (3/28);  total (17/50)
Fisher's exact test for biased strand distribution p-value = 5.56e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01

ATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTAGATATTGAAGCA  >  minE/347952‑348065
                                                              |                                                   
aTCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTACCGGCCAGGCACcgcgc                                               >  1:862272/1‑69 (MQ=255)
aTCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTACCGGCCAGGCACcgcgc                                               >  1:1713358/1‑69 (MQ=255)
aTCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTACCGGCCAGGCACcgcgc                                               >  1:1214744/1‑69 (MQ=255)
aTCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTACCGGCCAGGCACcgcgc                                               >  1:3434968/1‑69 (MQ=255)
aTCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTACCGGCCAGGCACcgcgc                                               >  1:438927/1‑69 (MQ=255)
aTCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTACCGGCCAGGCACcgcg                                                >  1:2211181/1‑68 (MQ=255)
    cctcgcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGccc                                           >  1:3396630/4‑69 (MQ=255)
    ccGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGccc                                           >  1:2377840/1‑69 (MQ=255)
    ccGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGccc                                           >  1:154316/1‑69 (MQ=255)
        gcTCTATTCCGCTTCTGCTTATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:944241/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCCTTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:423166/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:526654/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:2210789/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:89245/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:2429663/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:2539067/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:1863059/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:2893938/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:2988314/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:3093895/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:3247222/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:1175342/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:3303594/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:1190560/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:1634391/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:124529/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:374101/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:713137/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:1328911/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:516107/69‑1 (MQ=255)
        gcTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGGCTCCGCGCGCCCGTCt                                       <  1:664198/69‑1 (MQ=255)
         ctctATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTg                                      <  1:2545438/69‑1 (MQ=255)
         ctctATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTg                                      <  1:2336178/69‑1 (MQ=255)
                ccGCTTCTGCTGATTCCATTCCTATTCTGTGCATTACCGGCCAGGAACCGCGCGCCCGTCTGCATa                                  >  1:2143714/1‑66 (MQ=255)
                       tgctgATTCCATTCCTATTCTGTGTATTACCGGCCAGGCGCCGCGCGCCCGTCt                                       <  1:2297590/54‑1 (MQ=255)
                        gctgATTCCATTCCTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGAtt                         <  1:1612403/67‑1 (MQ=255)
                        gctgATTCCATTCCTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGAtt                         <  1:137277/67‑1 (MQ=255)
                        gctgATTCCATTCCTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGAtt                         <  1:1354799/67‑1 (MQ=255)
                        gctgATTCCATTCCTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGAtt                         <  1:1860908/67‑1 (MQ=255)
                        gctgATTCCATTCCTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGAtt                         <  1:1845665/67‑1 (MQ=255)
                           gATTCCATTCCTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCa                     >  1:2563352/1‑68 (MQ=255)
                           gATTCCATTCCTATTCTGAGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAg                    >  1:234339/1‑69 (MQ=255)
                                  ttccTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGttg             <  1:1106460/69‑3 (MQ=255)
                                  ttccTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTa              <  1:44659/68‑1 (MQ=255)
                                  ttccTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTa              <  1:1402606/68‑1 (MQ=255)
                                  ttccTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTa              <  1:163518/68‑1 (MQ=255)
                                  ttccTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTa              <  1:3324377/68‑1 (MQ=255)
                                  ttccTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTa              <  1:2757462/68‑1 (MQ=255)
                                  ttccTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTa              <  1:891655/68‑1 (MQ=255)
                                  ttccTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTa              <  1:1063216/68‑1 (MQ=255)
                                  ttccTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTa              <  1:2404720/68‑1 (MQ=255)
                                  ttccTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTAg             <  1:1813881/69‑1 (MQ=255)
                                  ttccTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTAg             <  1:18005/69‑1 (MQ=255)
                                   tccTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTAGa            <  1:1413261/69‑1 (MQ=255)
                                    ccTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTAGat           >  1:3186685/1‑69 (MQ=255)
                                    ccTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTAGat           >  1:928712/1‑69 (MQ=255)
                                    ccTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTAGat           >  1:1624777/1‑69 (MQ=255)
                                    ccTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTAGat           >  1:21366/1‑69 (MQ=255)
                                    ccTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTAGat           >  1:1022427/1‑69 (MQ=255)
                                       aTTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTAGatat         <  1:2337584/68‑1 (MQ=255)
                                               taTTACCGGCCAGGCTCCGCGCGCCCGTCTGCATAAAGAAGATTTCCAGGCCGTTGATATTGAAGc   <  1:1374288/65‑1 (MQ=255)
                                               taTTACCGGCCAGGCTCCGCGCGCCCGTCTGCATAAAGAAGATTTCCAGGCCGTTGATATTGAAGc   <  1:3402523/65‑1 (MQ=255)
                                               taTTACCGGCCAGGCTCCGCGCGCCCGTCTGCATAAAGAAGATTTCCAGGCCGTTGATATTGAAGc   <  1:1652334/65‑1 (MQ=255)
                                               taTTACCGGCCAGGCTCCGCGCGCCCGTCTGCATAAAGAAGATTTCCAGGCCGTTGATATTGAAGc   <  1:307407/65‑1 (MQ=255)
                                               taTTACCGGCCAGGCTCCGCGCGCCCGTCTGCATAAAGAAGATTTCCAGGCCGTTGATATTGAAGc   <  1:2010425/65‑1 (MQ=255)
                                               taTTACCGGCCAGGCTCCGCGCGCCCGTCTGCATAAAGAAGATTTCCAGGCCGTTGATATTGAAGc   <  1:1048375/65‑1 (MQ=255)
                                               cATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTAGATATTGAAGc   <  1:2009676/66‑1 (MQ=255)
                                                              aCCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTAGATATTGAAGCa  <  1:1262124/52‑1 (MQ=255)
                                                              aCCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTAGATATTGAAGCa  >  1:1976337/1‑52 (MQ=255)
                                                              |                                                   
ATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTAGATATTGAAGCA  >  minE/347952‑348065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑