| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 351,286 | A→T | 31.3% | G128G (GGT→GGA) | purK ← | N5‑carboxyaminoimidazole ribonucleotide synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 351,286 | 0 | A | T | 31.3% | 71.4 / 43.6 | 67 | G128G (GGT→GGA) | purK | N5‑carboxyaminoimidazole ribonucleotide synthase |
| Reads supporting (aligned to +/- strand): ref base A (11/35); new base T (16/5); total (27/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.02e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
TACATTCGCCGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGC > minE/351219‑351352 | tACATTCGCCGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGAc > 1:1930832/1‑69 (MQ=255)tACATTCGCCGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGAc > 1:796941/1‑69 (MQ=255)tACATTCGCCGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGAc > 1:2451658/1‑69 (MQ=255)tACATTCGCCGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGAc > 1:2507179/1‑69 (MQ=255)tACATTCGCCGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGAc > 1:182839/1‑69 (MQ=255)tACATTCGCCGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGAc > 1:279492/1‑69 (MQ=255)tACATTCGCCGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGACATTGACCGCGAc > 1:2051044/1‑69 (MQ=255)tACATTCGCCGTAACACTCTGCCGGTAACAGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGAc > 1:2706188/1‑69 (MQ=255) cGCCGTAGCATTCAGCAGGTAACTGTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGTCAt < 1:2757272/69‑1 (MQ=255) cGCCGTAGCATTCAGCAGGTAACTGTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGTCAt < 1:2780012/69‑1 (MQ=255) cGCCGTAGCATTCAGCAGGTAACTGTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGTCAt < 1:1596286/69‑1 (MQ=255) cGCCGTAGCATTCAGCAGGTAACTGTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGTCAt < 1:3193566/69‑1 (MQ=255) cGCCGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCAt < 1:3239612/69‑1 (MQ=255) gCCGTAGCATTCAGCAGGTAACTGTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGTCAt < 1:2293477/68‑1 (MQ=255) ccGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTTCGCGTAAACGCCATTGACCGCGACCGTCATa < 1:2532033/68‑1 (MQ=255) ccGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATa < 1:3134605/68‑1 (MQ=255) ccGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATa < 1:3224990/68‑1 (MQ=255) ccGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATa < 1:3251314/68‑1 (MQ=255) ccGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATa < 1:3397177/68‑1 (MQ=255) ccGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATa < 1:429586/68‑1 (MQ=255) ccGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATa < 1:461115/68‑1 (MQ=255) ccGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATa < 1:737158/68‑1 (MQ=255) ccGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATa < 1:806346/68‑1 (MQ=255) ccGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATa < 1:879667/68‑1 (MQ=255) ccGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATa < 1:2465022/68‑1 (MQ=255) ccGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATa < 1:1085674/68‑1 (MQ=255) ccGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATa < 1:139280/68‑1 (MQ=255) ccGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATa < 1:1432436/68‑1 (MQ=255) ccGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATa < 1:1643377/68‑1 (MQ=255) ccGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATa < 1:1894580/68‑1 (MQ=255) ccGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATa < 1:187543/68‑1 (MQ=255) ccGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGACATTGACCGCGACCGTCATa < 1:614350/68‑1 (MQ=255) ccGTAACACTCTGCCGGTAACTGTTCGGATTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATa < 1:219233/68‑1 (MQ=255) ccGTAACACTCTGCCGGTAACTGTTCGGATTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATa < 1:2657158/68‑1 (MQ=255) cGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATa < 1:1718491/67‑1 (MQ=255) ctctGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGt > 1:2359285/1‑69 (MQ=255) ctGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGc < 1:2847139/69‑1 (MQ=255) ctGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGc < 1:401565/69‑1 (MQ=255) ctGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGc < 1:1329288/69‑1 (MQ=255) ctGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGc < 1:2381452/69‑1 (MQ=255) gCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGc < 1:106759/67‑1 (MQ=255) gTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGt > 1:1684451/1‑42 (MQ=37) gTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGTCATAGCCACCAGTGCGGCGGTTAAc > 1:2506270/1‑67 (MQ=255) gTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGTCATAGCCACCAGTGCGGCGCTTaa > 1:1562334/1‑66 (MQ=255) gTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGTCATAGCCACCAGTGCGGCGCTTAAc > 1:2664765/1‑67 (MQ=255) gTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGTCATAGCCACCAGTGCGGCGCTTAAc > 1:3140078/1‑67 (MQ=255) gTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGTCATAGCCACCAGTGCGGCGCTTAACa > 1:1272807/1‑68 (MQ=255) gTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGTCATAGCCACCAGTGCGGCGCTTAACa > 1:2195853/1‑68 (MQ=255) gTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGTCATAGCCACCAGTGCGGCGCTTAACa > 1:1234713/1‑68 (MQ=255) gTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGTCATAGCCACCAGTGCGGCGCTTAACa > 1:2122539/1‑68 (MQ=255) gTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGTCATAGCCACCAGTGCGGCGCTTAACa > 1:1259342/1‑68 (MQ=255) gTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGTCATAGCCACCAGTGCGGCGCTTAACa > 1:2503493/1‑68 (MQ=255) gTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGTCATAGCCACCAGTGCGGCGCTTAACa > 1:3417717/1‑68 (MQ=255) gTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGTCATAGCCACCAGTGCGGCGCTTAACa > 1:1917195/1‑68 (MQ=255) gTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGTCATAGCCACCAGTGCGGCGCTTAACa > 1:1833971/1‑68 (MQ=255) gTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGTCATAGCCACCAGTGCGGCGCTTAACa > 1:274864/1‑68 (MQ=255) gTTCGGTTTCATCGGCGCGTAAACGCCATTGACCGCGTCCGTCATAGCCACCAGTGCGGCGCTTAACa > 1:1829828/1‑68 (MQ=255) gTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGc < 1:2550347/52‑1 (MQ=255) tttCATTTGCGCGTAAACGCCATTGACCGCGACCGTGATa < 1:742460/40‑1 (MQ=255) gcgTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTa < 1:865784/68‑1 (MQ=255) tAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTa < 1:2294527/65‑1 (MQ=255) cATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAac > 1:440462/1‑69 (MQ=255) gACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCAAGCTCACCTAAACGATCAAacac > 1:1250100/1‑67 (MQ=255) cgACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGc < 1:2413259/69‑1 (MQ=255) cgACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGc < 1:603868/69‑1 (MQ=255) cgACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGc < 1:115451/69‑1 (MQ=255) cgACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGc < 1:1761892/69‑1 (MQ=255) | TACATTCGCCGTAACACTCTGCCGGTAACTGTTCGGTTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGC > minE/351219‑351352 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |