| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 352,455 | C→T | 33.6% | Q187Q (CAG→CAA) | lpxH ← | UDP‑2,3‑diacylglucosamine pyrophosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 352,455 | 0 | C | T | 33.6% | 64.8 / 44.3 | 83 | Q187Q (CAG→CAA) | lpxH | UDP‑2,3‑diacylglucosamine pyrophosphatase |
| Reads supporting (aligned to +/- strand): ref base C (25/30); new base T (15/13); total (40/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
TGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTAC > minE/352390‑352519 | tGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGa > 1:233464/1‑69 (MQ=255)tGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGa > 1:355117/1‑69 (MQ=255)tGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGa > 1:368519/1‑69 (MQ=255)tGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGa > 1:2161243/1‑69 (MQ=255)tGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGa > 1:1703525/1‑69 (MQ=255)tGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGa > 1:2971904/1‑69 (MQ=255)tGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGa > 1:1584228/1‑69 (MQ=255)tGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGa > 1:1572293/1‑69 (MQ=255)tGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGa > 1:2911171/1‑69 (MQ=255)tGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGa > 1:660713/1‑69 (MQ=255)tGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGa > 1:1338909/1‑69 (MQ=255)tGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGa > 1:689433/1‑69 (MQ=255)tGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGa > 1:824120/1‑69 (MQ=255)tGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGa > 1:1125509/1‑69 (MQ=255)tGCTGATTGGCGATAAGTTCATGCACCGACGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGa > 1:187892/1‑69 (MQ=255) tAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGATGTTTTTCCATCg < 1:2351003/69‑1 (MQ=255) tAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGATGTTTTTCCATCg < 1:1042087/69‑1 (MQ=255) tAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGATGTTTTTCCATCg < 1:2392042/69‑1 (MQ=255) tAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGATGTTTTTCCATCg < 1:2601780/69‑1 (MQ=255) tAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGATGTTTTTCCATCg < 1:258635/69‑1 (MQ=255) tAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGATGTTTTTCCATCg < 1:2145286/69‑1 (MQ=255) tAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGATGTTTTTCCATCg < 1:2050340/69‑1 (MQ=255) tAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGATGTTTTTCCATCg < 1:2453035/69‑1 (MQ=255) tAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGATGTTTTTCCATCg < 1:2579340/69‑1 (MQ=255) tAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGATGTTTTTCCATCg < 1:1836907/69‑1 (MQ=255) tAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGATGTTTTTCCATCg < 1:807502/69‑1 (MQ=255) tAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGATGTTTTTCCATCg < 1:662418/69‑1 (MQ=255) tAAGTTCATGCAACGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACTTGATGTTTTTCCATCg < 1:1445575/69‑1 (MQ=255) aaGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGc < 1:3227603/69‑1 (MQ=255) aaGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGc < 1:505690/69‑1 (MQ=255) aaGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGc < 1:1726168/69‑1 (MQ=255) aaGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGc < 1:1120719/69‑1 (MQ=255) aaGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGc < 1:548842/69‑1 (MQ=255) ttCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACt > 1:2031118/1‑69 (MQ=255) ttCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACt > 1:2548919/1‑69 (MQ=255) ttCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACt > 1:2482277/1‑69 (MQ=255) ttCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACt > 1:1687941/1‑69 (MQ=255) ttCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACt > 1:3225051/1‑69 (MQ=255) ttCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATAGCACt > 1:302952/1‑69 (MQ=255) ttCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCAATCGCACt > 1:2844791/1‑69 (MQ=255) ttCATGCACCGACGGGCGATGGGTATGACCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACt > 1:101046/1‑69 (MQ=255) ttCATGAACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACt > 1:1447/1‑69 (MQ=255) tGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTcc > 1:990674/1‑69 (MQ=255) tGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTcc > 1:118268/1‑69 (MQ=255) tGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTcc > 1:2010698/1‑69 (MQ=255) tGCCAGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTcc > 1:1271611/1‑69 (MQ=255) cccGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCa > 1:559990/1‑68 (MQ=255) cGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATg < 1:2316138/68‑1 (MQ=255) gATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATg > 1:1125830/1‑64 (MQ=255) gATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATc > 1:2558225/1‑67 (MQ=255) gATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATc > 1:1541206/1‑67 (MQ=255) gATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATc > 1:1866575/1‑67 (MQ=255) gATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATc > 1:2181500/1‑67 (MQ=255) aTTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAtt < 1:591112/69‑1 (MQ=255) aTTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAtt < 1:2950927/69‑1 (MQ=255) aTTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAtt < 1:3014401/69‑1 (MQ=255) aTTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAtt < 1:310148/69‑1 (MQ=255) aTTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAtt < 1:1395545/69‑1 (MQ=255) aTTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAtt < 1:2378718/69‑1 (MQ=255) aTTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAtt < 1:3391095/69‑1 (MQ=255) aTTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAtt < 1:3407288/69‑1 (MQ=255) ttGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAttt < 1:2212914/69‑1 (MQ=255) ttGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAttt < 1:2712135/69‑1 (MQ=255) ttGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAtt < 1:633852/68‑1 (MQ=255) tGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTa > 1:446210/1‑69 (MQ=255) tGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTa > 1:1912277/1‑69 (MQ=255) tGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTa > 1:769863/1‑69 (MQ=255) tGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTa > 1:2410964/1‑69 (MQ=255) tGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTa > 1:3002980/1‑69 (MQ=255) gCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAttt < 1:2497992/67‑1 (MQ=255) gCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAttt < 1:414325/67‑1 (MQ=255) gCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAttt < 1:1151851/67‑1 (MQ=255) gCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAttt < 1:2520999/67‑1 (MQ=255) gCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAttt < 1:1117242/67‑1 (MQ=255) gCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAttt < 1:628796/67‑1 (MQ=255) gCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAttt < 1:2589283/67‑1 (MQ=255) gCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAttt < 1:1947943/67‑1 (MQ=255) gCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAttt < 1:2801302/67‑1 (MQ=255) gCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAttt < 1:3218079/67‑1 (MQ=255) gCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAttt < 1:3204245/67‑1 (MQ=255) gCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAttt < 1:2319048/67‑1 (MQ=255) gCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAttt < 1:2961581/67‑1 (MQ=255) gCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTAc > 1:2452455/1‑69 (MQ=255) | TGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTAC > minE/352390‑352519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |