| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 354,219 | A→G | 24.4% | P178P (CCA→CCG) | cysS → | cysteinyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 354,219 | 0 | A | G | 24.4% | 145.2 / 42.3 | 119 | P178P (CCA→CCG) | cysS | cysteinyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base A (50/39); new base G (17/12); total (68/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
AGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGG > minE/354158‑354279 | aGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATgg > 1:1670794/1‑66 (MQ=255)aGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATgg > 1:956290/1‑66 (MQ=255)aGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATgg > 1:244028/1‑66 (MQ=255)aGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATg > 1:1316307/1‑65 (MQ=255)aGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACt > 1:938568/1‑69 (MQ=255)aGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACt > 1:2240989/1‑69 (MQ=255)aGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACt > 1:135487/1‑69 (MQ=255)aGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACt > 1:203896/1‑69 (MQ=255)aGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACt > 1:1706227/1‑69 (MQ=255)aGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACt > 1:1000664/1‑69 (MQ=255) gCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:61843/69‑1 (MQ=255) gCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:325632/69‑1 (MQ=255) gCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:2797215/69‑1 (MQ=255) gCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:2555750/69‑1 (MQ=255) gCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:2174658/69‑1 (MQ=255) gCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:1796752/69‑1 (MQ=255) caggctggCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:1308686/65‑1 (MQ=255) caggcaggCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:1007797/65‑1 (MQ=255) caggcaggCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:1372126/65‑1 (MQ=255) caggcaggCGCGCGCGTTGACGTGGGCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:1472008/65‑1 (MQ=255) ggcaggcGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGt > 1:2166890/1‑68 (MQ=255) aggcGCGCGCGTTGACGTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTc > 1:97766/1‑66 (MQ=255) aggcGCGCGCGTTGACGTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTc > 1:1317385/1‑66 (MQ=255) aggcGCGCGCGTTGACGTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTc > 1:2531926/1‑66 (MQ=255) aggcGCGCGCGTTGACGTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTc > 1:185581/1‑66 (MQ=255) aggcGCGCGCGTTGACGTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTc > 1:653914/1‑66 (MQ=255) aggcGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTc > 1:330867/1‑66 (MQ=255) aggcGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTc > 1:106732/1‑66 (MQ=255) aggcGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTc > 1:635605/1‑66 (MQ=255) aggcGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTc > 1:730615/1‑66 (MQ=255) cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAAt > 1:1025390/1‑39 (MQ=255) cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATgg > 1:2469725/1‑41 (MQ=255) cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATg > 1:2500638/1‑40 (MQ=255) cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAaga > 1:2777846/1‑69 (MQ=255) cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAaga > 1:2547181/1‑69 (MQ=255) cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAaga > 1:312134/1‑69 (MQ=255) cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAaga > 1:36842/1‑69 (MQ=255) cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAaga > 1:3288541/1‑69 (MQ=255) cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAaga > 1:1836659/1‑69 (MQ=255) cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAaga > 1:1735512/1‑69 (MQ=255) cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAaga > 1:741292/1‑69 (MQ=255) cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAaga > 1:747763/1‑69 (MQ=255) gACGTGGTCGACGACAAACGCAACCCAATGGACtt > 1:2429038/1‑35 (MQ=255) gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGtt > 1:2624533/1‑39 (MQ=255) gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTc > 1:2683209/1‑40 (MQ=255) gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTc > 1:3084166/1‑40 (MQ=255) gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTc > 1:549147/1‑40 (MQ=255) gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTc > 1:1250210/1‑40 (MQ=255) gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTc > 1:736082/1‑40 (MQ=255) gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTc > 1:1049578/1‑40 (MQ=255) gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTc > 1:1550898/1‑40 (MQ=255) gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTc > 1:2317606/1‑40 (MQ=255) gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTc > 1:1948838/1‑40 (MQ=255) gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTc > 1:1357475/1‑40 (MQ=255) gTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc > 1:3171272/1‑69 (MQ=255) gTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc > 1:253220/1‑69 (MQ=255) gTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc > 1:3103028/1‑69 (MQ=255) gTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc > 1:2583872/1‑69 (MQ=255) gTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc > 1:1323459/1‑69 (MQ=255) gTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc > 1:1385910/1‑69 (MQ=255) gTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc > 1:271400/1‑69 (MQ=255) gTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc > 1:2854343/1‑69 (MQ=255) gTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:1718131/44‑1 (MQ=255) ggTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTg < 1:2382000/69‑1 (MQ=255) ggTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTg < 1:2723788/69‑1 (MQ=255) gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:1753295/60‑1 (MQ=255) gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:1686094/60‑1 (MQ=255) gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:1812161/60‑1 (MQ=255) gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:811256/60‑1 (MQ=255) gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:342903/60‑1 (MQ=255) gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:2095796/60‑1 (MQ=255) gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:1673709/60‑1 (MQ=255) gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:3270814/60‑1 (MQ=255) gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:1670195/60‑1 (MQ=255) gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:1084824/60‑1 (MQ=255) gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:3095181/60‑1 (MQ=255) gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:230083/60‑1 (MQ=255) gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:2975167/60‑1 (MQ=255) gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTgg > 1:2176943/1‑69 (MQ=255) gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTgg > 1:864206/1‑69 (MQ=255) gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTgg > 1:1962489/1‑69 (MQ=255) gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTgg > 1:298108/1‑69 (MQ=255) gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTg < 1:1785776/68‑1 (MQ=255) tCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:2953829/59‑1 (MQ=255) tCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:1924824/59‑1 (MQ=255) acAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTg < 1:524019/61‑1 (MQ=255) acAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGtctc < 1:2082216/69‑1 (MQ=255) cAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGtctc < 1:559802/68‑1 (MQ=255) cGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTgg > 1:1297734/1‑69 (MQ=255) aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:2387406/50‑1 (MQ=255) aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:83637/50‑1 (MQ=255) aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:2339712/50‑1 (MQ=255) aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:3246438/50‑1 (MQ=255) aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:1648309/50‑1 (MQ=255) aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:2988961/50‑1 (MQ=255) aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:407226/50‑1 (MQ=255) aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:347277/50‑1 (MQ=255) aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:2150937/50‑1 (MQ=255) aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:3422843/50‑1 (MQ=255) aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:211268/50‑1 (MQ=255) | AGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGG > minE/354158‑354279 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |