| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 354,519 | C→T | 38.7% | R278R (CGC→CGT) | cysS → | cysteinyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 354,519 | 0 | C | T | 38.7% | 45.9 / 53.0 | 85 | R278R (CGC→CGT) | cysS | cysteinyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (14/38); new base T (19/14); total (33/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.33e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTAT > minE/354451‑354579 | cACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTgcgc < 1:2687502/69‑1 (MQ=255)cACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTgcgc > 1:2440654/1‑69 (MQ=255)cACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTgcgc > 1:2261986/1‑69 (MQ=255)cACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTgcgc > 1:883165/1‑69 (MQ=255)cACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTgcgc < 1:1831887/69‑1 (MQ=255) gatggtgatggtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGc > 1:3434034/1‑68 (MQ=255) gtgatggtTGACCGCGAGAAGATGTCCAAATCGCTTGGTAACTTCTTTACCGTGCGCGATGTGCTGaaa < 1:2064157/69‑1 (MQ=255) gtgatggtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGGTGaaa < 1:2625940/69‑1 (MQ=255) gtgatggtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGaaa < 1:998890/69‑1 (MQ=255) gtgatggtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGaaa < 1:2206775/69‑1 (MQ=255) gtgatggtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGaaa < 1:2186396/69‑1 (MQ=255) gtgatggtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGaaa < 1:3434243/69‑1 (MQ=255) gtgatggtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGaaa < 1:371494/69‑1 (MQ=255) gtgatggtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGaaa < 1:1639643/69‑1 (MQ=255) gtgatggtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGaaa < 1:1208413/69‑1 (MQ=255) gtgatggtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGaaa < 1:1428794/69‑1 (MQ=255) gatggtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGaaa < 1:3065381/67‑1 (MQ=255) attgtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAAtac < 1:2101674/66‑1 (MQ=255) atggtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAAtac < 1:3269909/69‑1 (MQ=255) atggtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAAtac < 1:2818854/69‑1 (MQ=255) atggtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAAtac < 1:3154716/69‑1 (MQ=255) atggtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAAtac < 1:1285522/69‑1 (MQ=255) atggtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAAtac < 1:825544/69‑1 (MQ=255) tggtTGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAtac > 1:975862/1‑68 (MQ=255) tggtTGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAtac > 1:1623802/1‑68 (MQ=255) tggtTGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAtac > 1:1372647/1‑68 (MQ=255) tggtTGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAtac > 1:3084287/1‑68 (MQ=255) tggtTGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAtac > 1:2064074/1‑68 (MQ=255) tggtTGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAtac > 1:2289937/1‑68 (MQ=255) tggtTGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAtac > 1:3013652/1‑68 (MQ=255) tggtTGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAtac > 1:2802933/1‑68 (MQ=255) tggtTGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAtac > 1:2782569/1‑68 (MQ=255) tggtTGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAtac > 1:2769218/1‑68 (MQ=255) tggtTGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAtac > 1:3327219/1‑68 (MQ=255) tggtTGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAtac > 1:450180/1‑68 (MQ=255) tggtTGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAtac > 1:1704700/1‑68 (MQ=255) tggtTGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAtac > 1:3393803/1‑68 (MQ=255) tggtTGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAtac > 1:1724244/1‑68 (MQ=255) tggtTGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAtac > 1:1560094/1‑68 (MQ=255) tggtTGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAtac > 1:346634/1‑68 (MQ=255) tggtTGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAtac > 1:1791371/1‑68 (MQ=255) tggtTGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAtac > 1:433740/1‑68 (MQ=255) gtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAAtactac > 1:1271981/1‑69 (MQ=255) tgcccGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGaaa < 1:12639/58‑1 (MQ=255) tGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAta < 1:798050/63‑1 (MQ=255) tGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAta < 1:3304961/63‑1 (MQ=255) tGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAta < 1:3426158/63‑1 (MQ=255) tGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAta < 1:439861/63‑1 (MQ=255) tGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAta < 1:3369772/63‑1 (MQ=255) tGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAta < 1:3219873/63‑1 (MQ=255) tGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAta < 1:1191972/63‑1 (MQ=255) tGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAta < 1:630835/63‑1 (MQ=255) tGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAta < 1:1145980/63‑1 (MQ=255) tGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAta < 1:1438285/63‑1 (MQ=255) tGACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAta < 1:2139808/63‑1 (MQ=255) gACCGCGAGAAGATGTCTAAATCGCTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAta < 1:1936163/62‑1 (MQ=255) aTGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTg > 1:1303297/1‑69 (MQ=255) aTGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTg > 1:1580406/1‑69 (MQ=255) cAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGtt < 1:2073716/68‑1 (MQ=255) gCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGtt > 1:1775102/1‑62 (MQ=255) gCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGtt > 1:2377693/1‑62 (MQ=255) gCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGtt > 1:1663877/1‑62 (MQ=255) gCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGtt > 1:658294/1‑62 (MQ=255) gCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCt < 1:2008539/69‑1 (MQ=255) gCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCt < 1:428542/69‑1 (MQ=255) gCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCt < 1:2653220/69‑1 (MQ=255) gCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCt < 1:1838746/69‑1 (MQ=255) gCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCt < 1:1971512/69‑1 (MQ=255) gCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCt < 1:2903534/69‑1 (MQ=255) gCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCt < 1:329321/69‑1 (MQ=255) gCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCt < 1:3255532/69‑1 (MQ=255) gCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCt < 1:2139751/69‑1 (MQ=255) gCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCt < 1:1286440/69‑1 (MQ=255) gCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCt < 1:2303165/69‑1 (MQ=255) cTGGGTAACTTCTTCACCGTGCGTGATGTGCTGAAAta < 1:1828409/38‑1 (MQ=38) gggTAACTTCTTCACCGTGCGTGATGTGCTGAAAta < 1:2142185/36‑1 (MQ=37) gTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCt < 1:1937059/64‑1 (MQ=255) ttcttTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGc < 1:180755/69‑1 (MQ=255) ttcttTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGc < 1:1838335/69‑1 (MQ=255) ttcttTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGc < 1:677788/69‑1 (MQ=255) ttcttTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGc < 1:1573101/69‑1 (MQ=255) tcttTACCGTGCGCGATGTGCTTAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGcc < 1:745480/69‑1 (MQ=255) tcttTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGcc < 1:1706025/69‑1 (MQ=255) tcttTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGcc < 1:681498/69‑1 (MQ=255) cGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTAt > 1:466793/1‑67 (MQ=255) cGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTAt > 1:629064/1‑67 (MQ=255) cGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTAt > 1:2051529/1‑67 (MQ=255) cGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTAt > 1:318684/1‑67 (MQ=255) cGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTAt > 1:93128/1‑67 (MQ=255) cGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCATGATGTCGGGCCACTAt > 1:1731332/1‑67 (MQ=255) cgcgATGTGCTGAAATACTACGACGCGGAAACCGGGCGTTACTTCCt < 1:2583363/47‑1 (MQ=255) | CACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTAT > minE/354451‑354579 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |