| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 355,392 | G→A | 36.5% | R79R (CGC→CGT) | ybcI ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 355,392 | 0 | G | A | 36.5% | 73.9 / 68.0 | 112 | R79R (CGC→CGT) | ybcI | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (39/32); new base A (10/31); total (49/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.79e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
Only 100 of 115 total aligned reads displayed.
GCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGAT > minE/355331‑355459 | gCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCa > 1:2154970/1‑68 (MQ=255)gCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCa > 1:825328/1‑68 (MQ=255)gCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCa > 1:2375138/1‑68 (MQ=255)gCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCa > 1:2717887/1‑68 (MQ=255)gCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCa > 1:1574160/1‑68 (MQ=255)gCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCa > 1:1553083/1‑68 (MQ=255)gCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCa > 1:1272379/1‑68 (MQ=255)gCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCa > 1:2871851/1‑68 (MQ=255) aGCAACTAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCTGCGTCCAATa > 1:2110406/1‑66 (MQ=255) aGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAAt > 1:2967291/1‑65 (MQ=255) aGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATa > 1:1052548/1‑66 (MQ=255) aGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATa > 1:3279862/1‑66 (MQ=255) aGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATa > 1:3378580/1‑66 (MQ=255) aGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATa > 1:354367/1‑66 (MQ=255) aGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATa > 1:56339/1‑66 (MQ=255) aGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATa > 1:595032/1‑66 (MQ=255) aGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATa > 1:1151225/1‑66 (MQ=255) aGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCAAGCCCTGAACCATCGGCGTCCAATa > 1:367962/1‑66 (MQ=255) gTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGc < 1:299510/69‑1 (MQ=255) gTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGc < 1:365872/69‑1 (MQ=255) gTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGc < 1:2684555/69‑1 (MQ=255) cagccagcAGCGAATCAGCCCAGCCCTGAACCATCTGCGTCCAATAAACACACATAACAGCGGGacaa > 1:945255/1‑68 (MQ=255) cagccagcAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGacaa > 1:821537/1‑68 (MQ=255) cagccagcAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGacaa > 1:1823988/1‑68 (MQ=255) cagccagcAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGacaa > 1:22232/1‑68 (MQ=255) cagccagcAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGacaa > 1:2281081/1‑68 (MQ=255) cagccagcAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGacaa > 1:295481/1‑68 (MQ=255) cagccagcAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGAGGGacaa > 1:2717976/1‑68 (MQ=255) agccagcAGCGAATCAGCCCAGCCCTGAACCATCGGCGTc > 1:2647563/1‑40 (MQ=255) agccagcAGCGAATCAGCCCAGCCCTGAACCATCGGCGTc > 1:3407467/1‑40 (MQ=255) agccagcAGCGAATCAGCCCAGCCCTGAACCATCGGCGTc > 1:970866/1‑40 (MQ=255) agccagcAGCGAATCAGCCCAGCCCTGAACCATCGGCGTc > 1:1978156/1‑40 (MQ=255) agccagcAGCGAATCAGCCCAGCCCTGAACCATCGGCGTc > 1:1694797/1‑40 (MQ=255) cagcGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCa < 1:2793965/69‑1 (MQ=255) cagcGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCa < 1:1581880/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:1551874/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:2759016/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:2913458/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:3410138/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:1507517/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:3178106/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:640676/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:2271315/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:1356096/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:253660/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:241006/69‑1 (MQ=255) gcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:400782/68‑1 (MQ=255) cGAATCAGCCCAGCCCCGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCaaa < 1:1151339/68‑1 (MQ=255) cccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCg < 1:1322520/68‑1 (MQ=255) cccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCg < 1:1318531/68‑1 (MQ=255) cccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCg < 1:3187108/68‑1 (MQ=255) cccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCg < 1:1500659/68‑1 (MQ=255) cccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCg < 1:3305325/68‑1 (MQ=255) cccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCg < 1:3348888/68‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa < 1:1550844/69‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa < 1:3274121/69‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa < 1:396411/69‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa < 1:1944302/69‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa < 1:2760963/69‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa > 1:9342/1‑69 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa > 1:1808454/1‑69 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa < 1:809405/69‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa > 1:660295/1‑69 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa < 1:17616/69‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa > 1:638704/1‑69 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa > 1:2335183/1‑69 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa < 1:2578229/69‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa < 1:117119/69‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa < 1:1236808/69‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa < 1:1634465/69‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa < 1:1609668/69‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa > 1:448348/1‑69 (MQ=255) agcgcTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACATACGCAAACACCAGCGAATg < 1:381270/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGGACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:300607/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:1057201/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:1008453/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:1386381/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:326118/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:3052067/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:3050557/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:2117549/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:2898222/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:1663231/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:2176844/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:1704614/69‑1 (MQ=255) ccTGAACCATCGGCGTCCAATAAACACACATAACTGc < 1:388916/37‑1 (MQ=255) cTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCg < 1:2259550/61‑1 (MQ=255) cTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTa > 1:2052772/1‑69 (MQ=255) cTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTa > 1:1409956/1‑69 (MQ=255) cTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTa > 1:1143590/1‑69 (MQ=255) cTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTa > 1:2153381/1‑69 (MQ=255) cTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTa > 1:635614/1‑69 (MQ=255) cTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTa > 1:2764792/1‑69 (MQ=255) cTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTa > 1:2782062/1‑69 (MQ=255) cTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTa > 1:2986999/1‑69 (MQ=255) gAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTaaa < 1:2603722/69‑1 (MQ=255) gAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTaaa < 1:1502922/69‑1 (MQ=255) ggCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGAt < 1:2210913/69‑1 (MQ=255) ggCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGAt < 1:587569/69‑1 (MQ=255) ggCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGAt < 1:1892025/69‑1 (MQ=255) | GCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGAT > minE/355331‑355459 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |