| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 355,403 | A→G | 44.1% | F76L (TTT→CTT) ‡ | ybcI ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 355,403 | 0 | A | G | 44.1% | 18.3 / 75.0 | 93 | F76L (TTT→CTT) ‡ | ybcI | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (17/35); new base G (10/31); total (27/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 121 total aligned reads displayed.
GAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACA > minE/355342‑355470 | gagaCGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAAGCATCGGCGTCCAATAAACACACAt < 1:207352/69‑1 (MQ=255) gTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGc < 1:2684555/69‑1 (MQ=255) gTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGc < 1:2219617/69‑1 (MQ=255) gTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGc < 1:365872/69‑1 (MQ=255) cagccagcAGCGAATCAGCCCAGCCCTGAACCATCTGCGTCCAATAAACACACATAACAGCGGGacaa > 1:945255/1‑68 (MQ=255) cagccagcAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGacaa > 1:700607/1‑68 (MQ=255) cagccagcAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGacaa > 1:295481/1‑68 (MQ=255) cagccagcAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGacaa > 1:22232/1‑68 (MQ=255) cagccagcAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGacaa > 1:2281081/1‑68 (MQ=255) cagccagcAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGacaa > 1:1823988/1‑68 (MQ=255) cagccagcAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGacaa > 1:821537/1‑68 (MQ=255) cagccagcAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGacaa > 1:2751018/1‑68 (MQ=255) cagccagcAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGAGGGacaa > 1:2717976/1‑68 (MQ=255) cagcGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCa < 1:1581880/69‑1 (MQ=255) cagcGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCa < 1:2793965/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:2913458/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:1356096/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:1507517/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:2759016/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:1551874/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:241006/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:3011248/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:3410138/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:640676/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:2271315/69‑1 (MQ=255) agcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:253660/69‑1 (MQ=255) gcGAATCAGCCCAGCCCTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCaa < 1:400782/68‑1 (MQ=255) cGAATCAGCCCAGCCCCGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCaaa < 1:1151339/68‑1 (MQ=255) cccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACATCAAACGCAAACACCAGCg < 1:1743274/68‑1 (MQ=255) cccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCg < 1:3305325/68‑1 (MQ=255) cccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCg < 1:3187108/68‑1 (MQ=255) cccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCg < 1:3348888/68‑1 (MQ=255) cccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCg < 1:1322520/68‑1 (MQ=255) cccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCg < 1:1500659/68‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCg < 1:545085/67‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa < 1:2578229/69‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa > 1:2335183/1‑69 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa > 1:9342/1‑69 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa > 1:660295/1‑69 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa < 1:1944302/69‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa < 1:3274121/69‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa < 1:17616/69‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa < 1:1634465/69‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa < 1:1609668/69‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa < 1:1550844/69‑1 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa > 1:448348/1‑69 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa > 1:638704/1‑69 (MQ=255) ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGaa < 1:1236808/69‑1 (MQ=255) agcgcTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACATACGCAAACACCAGCGAATg < 1:381270/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGGACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:300607/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:3050557/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:2898222/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:3052067/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:1057201/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:1008453/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:2488968/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:1704614/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:1663231/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:1386381/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:2117549/69‑1 (MQ=255) agcccTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATg < 1:2176844/69‑1 (MQ=255) ccTGAACCATCGGCGTCCAATAAACACACATAACTGc < 1:388916/37‑1 (MQ=255) cTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCg < 1:2259550/61‑1 (MQ=255) cTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAATCGCAAACACCAGCGAATGGGTa > 1:3286567/1‑69 (MQ=255) cTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTa > 1:2986999/1‑69 (MQ=255) cTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTa > 1:2153381/1‑69 (MQ=255) cTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTa > 1:635614/1‑69 (MQ=255) cTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTa > 1:1209681/1‑69 (MQ=255) cTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTa > 1:1409956/1‑69 (MQ=255) cTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTa > 1:2782062/1‑69 (MQ=255) cTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTa > 1:2764792/1‑69 (MQ=255) cTGAACCATCGACGTCCAATCAGCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTa > 1:1143590/1‑69 (MQ=255) gAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTaaa < 1:1502922/69‑1 (MQ=255) ggCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGAt < 1:2210913/69‑1 (MQ=255) ggCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGAt < 1:1892025/69‑1 (MQ=255) cAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCa > 1:1264413/1‑68 (MQ=255) aaaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGa < 1:121379/61‑1 (MQ=255) aaaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGa < 1:185176/61‑1 (MQ=255) aaaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGa < 1:3245958/61‑1 (MQ=255) aaaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGa < 1:245691/61‑1 (MQ=255) aaaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGa < 1:1866039/61‑1 (MQ=255) aaaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAAc < 1:2817212/69‑1 (MQ=255) aaaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAAc < 1:1235494/69‑1 (MQ=255) aaaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAAc < 1:1356354/69‑1 (MQ=255) aaaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAAc < 1:90868/69‑1 (MQ=255) aaaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAAc < 1:487824/69‑1 (MQ=255) aaaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAAc < 1:3092043/69‑1 (MQ=255) aaaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAAc < 1:2665004/69‑1 (MQ=255) aaaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAAc < 1:3221712/69‑1 (MQ=255) aaaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAAc < 1:3379812/69‑1 (MQ=255) aaaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAAc < 1:1754175/69‑1 (MQ=255) aaaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAAc < 1:1784441/69‑1 (MQ=255) aaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCaaaaa > 1:2338455/1‑67 (MQ=255) aaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACa > 1:1158090/1‑69 (MQ=255) aaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACa > 1:2528634/1‑69 (MQ=255) aaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACa > 1:2901486/1‑69 (MQ=255) aaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACa > 1:2074633/1‑69 (MQ=255) aaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACa > 1:516766/1‑69 (MQ=255) aaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACa > 1:3216421/1‑69 (MQ=255) aaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACa > 1:3217027/1‑69 (MQ=255) | GAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACA > minE/355342‑355470 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |