| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 355,755 | C→G | 49.1% | S65T (AGC→ACC) | ybcJ ← | predicted RNA‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 355,755 | 0 | C | G | 49.1% | ‑2.3 / 63.9 | 53 | S65T (AGC→ACC) | ybcJ | predicted RNA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (19/8); new base G (21/5); total (40/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGC > minE/355687‑355819 | cAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGc > 1:2803833/1‑69 (MQ=255) cTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGAcc < 1:3352512/69‑1 (MQ=255) agagAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCtgtg < 1:2203642/60‑1 (MQ=255) agagAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCtgtg < 1:1226111/60‑1 (MQ=255) agagAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCtgtg < 1:2187109/60‑1 (MQ=255) agagAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCtgtg < 1:3411872/60‑1 (MQ=255) aCCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTc < 1:3017597/68‑1 (MQ=255) ggCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCaa > 1:1741944/1‑60 (MQ=255) ggCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCaa > 1:583547/1‑60 (MQ=255) ggCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCaa > 1:2460893/1‑60 (MQ=255) ggCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCaa > 1:3411830/1‑60 (MQ=255) ggCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCaa > 1:338332/1‑60 (MQ=255) ggCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCaa > 1:2052984/1‑60 (MQ=255) ggCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCaa > 1:1518944/1‑60 (MQ=255) ggCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCaa > 1:3043646/1‑60 (MQ=255) ggCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCaa > 1:3020728/1‑60 (MQ=255) gCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGGTGTGACCTGCaa > 1:2804041/1‑59 (MQ=255) gCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGGTGTGACCTGCaa > 1:2611145/1‑59 (MQ=255) gCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGGTGTGACCTGCaa > 1:1019049/1‑59 (MQ=255) gCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGGTGTGACCTGCaa > 1:273216/1‑59 (MQ=255) gCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGGTGTGACCTGCaa > 1:1766456/1‑59 (MQ=255) gCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGGTGTGACCTGCaa > 1:790417/1‑59 (MQ=255) gCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGGTGTGACCTGCaa > 1:1384519/1‑59 (MQ=255) gCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGGTGTGACCTGCaa > 1:1032077/1‑59 (MQ=255) gATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCg > 1:2812640/1‑69 (MQ=255) gATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCg > 1:860499/1‑69 (MQ=255) gATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCg > 1:2750624/1‑69 (MQ=255) gATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCg > 1:254914/1‑69 (MQ=255) gATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCg > 1:1635881/1‑69 (MQ=255) aCAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCAGTGTCTGACCGGc < 1:927083/68‑1 (MQ=255) aCAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGc < 1:3214766/68‑1 (MQ=255) aaGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCa > 1:1827342/1‑69 (MQ=255) gATCAGTCAACAACCTGTACGGTGTGACCTGCAAAACTCACTGTCTGACCGGCGACAATTTTGCAGCGt < 1:2272604/69‑1 (MQ=255) gATCAGGCAACAACCTGTACGGTGTGACCTGCAAAAGTCACTGTCTGACCGGCGACAATTTTGCAGCGt < 1:2623015/69‑1 (MQ=255) gATCAGGCAACAACCTGTACGGTGTGACCTGCAAAACTCACTGTCTGACCGGCGACAATTTTGCAGCGt < 1:2862691/69‑1 (MQ=255) gATCAGGCAACAACCTGTACGGTGTGACCTGCAAAACTCACTGTCTGACCGGCGACAATTTTGCAGCGt < 1:2154306/69‑1 (MQ=255) gATCAGGCAACAACCTGTACGGTGTGACCTGCAAAACTAACTGTCTGACCGGCGACAATTTTGCAGCGt < 1:1996647/69‑1 (MQ=255) tCAGGCAACAACCTGTACGGTGTGACCTGCAAAACTCACTGTCTGACCGGcgac > 1:531737/1‑54 (MQ=255) tCAGGCAACAACCTGTACGGTGTGACCTGCAAAACTCACTGTCTGACCGGCGACAATTTTGCAGCGttt > 1:2342259/1‑69 (MQ=255) tCAGGCAACAACCTGTACGGTGTGACCTGCAAAACTCACTGTCTGACCGGCGACAATTTTGCAGCGttt > 1:969996/1‑69 (MQ=255) tCAGGCAACAACCTGTACGGTGTGACCTGCAAAACTCACTGTCTGACCGGCGACAATTTTGCAGCGttt > 1:2462635/1‑69 (MQ=255) tCAGGCAACAACCTGTACGGTGTGACCTGCAAAACTCACTGTCTGACCGGCGACAATTTTGCAGCGttt > 1:2939289/1‑69 (MQ=255) tCAGGCAACAACCTGTACGGTGTGACCTGCAAAACTCACTGTCTGACCGGCGACAATTTTGCAGCGttt > 1:781122/1‑69 (MQ=255) tCAGGCAACAACCTGTACGGTGTGACCTGCAAAACTCACTGTCTGACCGGCGACAATTTTGCAGCGttt > 1:670669/1‑69 (MQ=255) tCAGGCAACAACCTGTACGGTGTGACCTGCAAAACTCACTGTCTGACCGGCGACAATTTTGCAGCGttt > 1:584122/1‑69 (MQ=255) tCAGGCAACAACCTGTACGGTGTGACCTGCAAAACTCACTGTCTGACCGGCGACAATTTTGCAGCGttt > 1:530836/1‑69 (MQ=255) tCAGGCAACAACCTGTACGGTGTGACCTGCAAAACTCACTGTCTGACCGGCGACAATTTTGCAGCGttt > 1:455004/1‑69 (MQ=255) tCAGGCAACAACCTGTACGGTGTGACCTGCAAAACTCACTGTCTGACCGGCGACAATTTTGCAGCGttt > 1:2458692/1‑69 (MQ=255) tCAGGCAACAACCTGTACGGTGTGACCTGCAAAACTCACTGTCTGACCGGCGACAATTTTGCAGCGttt > 1:2665598/1‑69 (MQ=255) tCAGGCAACAACCTGTACGGTGTGACCTGCAAAACTCACTGTCTGACCGGCGACAATTTTGCAGCGttt > 1:2018057/1‑69 (MQ=255) aacCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTc > 1:1761742/1‑69 (MQ=255) aacCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTc > 1:922190/1‑69 (MQ=255) aacCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTc > 1:3318142/1‑69 (MQ=255) tACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGc > 1:1192828/1‑69 (MQ=255) | CAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGC > minE/355687‑355819 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |