| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 364,740 | G→C | 52.5% | V82V (GTC→GTG) | entD ← | phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 364,740 | 0 | G | C | 52.5% | 0.4 / 45.9 | 40 | V82V (GTC→GTG) | entD | phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex |
| Reads supporting (aligned to +/- strand): ref base G (5/14); new base C (19/2); total (24/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.69e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01 | |||||||||||
GTAGTCCCACAGTGGCTAATACTGCCGTATACCTCCGCAGGCCAGACAGGTTGGCGTAGCTCGCCGATTGCGGGCACACATTTATAGCCATATTCCCGCAAAGCATA > minE/364696‑364802 | gTAGTCCCACAGTGGCTAATACTGCCGTATACCTCCGCAGGCCAGACAGGTTGGCGTAGCTCGCCGAtt > 1:868267/1‑69 (MQ=255) gTCCCACAGTGACTAATGCTGCCGTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGCCGATTGca < 1:842280/69‑2 (MQ=255) gTCCCACAGTGACTAATGCTGCCGTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGCCGATTGca < 1:2747059/69‑2 (MQ=255) ggCTAATACTGCCGTATACCTCCGCAGGCCAGACAGGTTGGCGTAGCTCGCCGATTGCGGGCACACAtt < 1:3050386/69‑1 (MQ=255) ggCTAATACTGCCGTATACCTCCGCAGGCCAGACAGGTTGGCGTAGCTCGCCGATTGCGGGCACACAtt > 1:3345122/1‑69 (MQ=255) gCTAATACTGCCGTATACCTCCGCAGGCCAGACAGGTTGGCGTAGCTCGCCGATTGCGGGCACACAtt < 1:3231182/68‑1 (MQ=255) cTGCCGTATACCTCCGCAGGCCAGACAGGTTGGCGTAGCTCGCCGATTGCGGGCACACATTTATAGCCa < 1:924579/69‑1 (MQ=255) cTGCCGTATACCTCCGCAGGCCAGACAGGTTGGCGTAGCTCGCCGATTGCGGGCACACATTTATAGCCa < 1:1718848/69‑1 (MQ=255) gTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGcc > 1:907978/1‑39 (MQ=38) gTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGCCg > 1:2723589/1‑40 (MQ=255) gTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGCCg > 1:1324089/1‑40 (MQ=255) gTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGCCg > 1:2747654/1‑40 (MQ=255) gTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGCCg > 1:501845/1‑40 (MQ=255) gTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGCCg > 1:535365/1‑40 (MQ=255) gTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGCCg > 1:547107/1‑40 (MQ=255) gTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGCCg > 1:611268/1‑40 (MQ=255) gTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGCCg > 1:627802/1‑40 (MQ=255) gTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGCCg > 1:1307723/1‑40 (MQ=255) gTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGCCg > 1:2412729/1‑40 (MQ=255) gTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGCCg > 1:1398994/1‑40 (MQ=255) gTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGCCg > 1:1538609/1‑40 (MQ=255) gTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGCCg > 1:1611816/1‑40 (MQ=255) gTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGCCg > 1:171937/1‑40 (MQ=255) gTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGCCg > 1:2029426/1‑40 (MQ=255) gTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGCCg > 1:2608165/1‑40 (MQ=255) gTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGCCg > 1:1873060/1‑40 (MQ=255) gTATACCCCCGCAGGCCACACAGGTTGGCGTAGCTCGCCg > 1:1756182/1‑40 (MQ=255) ccTCCGCAGGCCAGACAGGTTGGCGTAGCTCGCCGATTGCGGGCACACATTTATAGCCATATTCCCg > 1:2796758/1‑67 (MQ=255) ccTCCGCAGGCCAGACAGGTTGGCGTAGCTCGCCGATTGCGGGCACACATTTATAGCCATATTCCCg > 1:2828972/1‑67 (MQ=255) tCCGCAGGCCAGACAGGTTGGCGTAGCTCGCCGATTGCGGGCACACATTTATAGCCATATTCCCGCaa > 1:975566/1‑68 (MQ=255) ccGCAGGCCAGACAGGTTGGCGTAGCTCGCCGATTGCGGGCACACATTTATAGCCATATTCCCGCAAAg < 1:1937173/69‑1 (MQ=255) aGGCCAGACAGGTTGGCGTAGCTCGCCGATTGCGGGCACACATTTATAGCCATATTCCCGCAAAGCATa < 1:365064/69‑1 (MQ=255) aGGCCAGACAGGTTGGCGTAGCTCGCCGATTGCGGGCACACATTTATAGCCATATTCCCGCAAAGCATa < 1:583609/69‑1 (MQ=255) aGGCCAGACAGGTTGGCGTAGCTCGCCGATTGCGGGCACACATTTATAGCCATATTCCCGCAAAGCATa < 1:1836675/69‑1 (MQ=255) aGGCCAGACAGGTTGGCGTAGCTCGCCGATTGCGGGCACACATTTATAGCCATATTCCCGCAAAGCATa < 1:218543/69‑1 (MQ=255) aGGCCAGACAGGTTGGCGTAGCTCGCCGATTGCGGGCACACATTTATAGCCATATTCCCGCAAAGCATa < 1:3224092/69‑1 (MQ=255) aGGCCAGACAGGTTGGCGTAGCTCGCCGATTGCGGGCACACATTTATAGCCATATTCCCGCAAAGCATa < 1:2324045/69‑1 (MQ=255) aGGCCAGACAGGTTGGCGTAGCTCGCCGATTGCGGGCACACATTTATAGCCATATTCCCGCAAAGCATa < 1:2995032/69‑1 (MQ=255) aGGCCAGACAGGTTGGCGTAGCTCGCCGATTGCGGGCACACATTTATAGCCATATTCCCGCAAAGCATa < 1:2434770/69‑1 (MQ=255) aGGCCAGACAGGTTGGCGTAGCTCGCCGATTGCGGGCACACATTTATAGCCATATTCCCGCAAAGCATa < 1:2605144/69‑1 (MQ=255) | GTAGTCCCACAGTGGCTAATACTGCCGTATACCTCCGCAGGCCAGACAGGTTGGCGTAGCTCGCCGATTGCGGGCACACATTTATAGCCATATTCCCGCAAAGCATA > minE/364696‑364802 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |