Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 365,118 | G→T | 35.7% | intergenic (‑133/+33) | entD ← / ← fepA | phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex/iron‑enterobactin outer membrane transporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 365,118 | 0 | G | T | 35.7% | 87.2 / 60.8 | 84 | intergenic (‑133/+33) | entD/fepA | phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex/iron‑enterobactin outer membrane transporter |
Reads supporting (aligned to +/- strand): ref base G (29/25); new base T (18/12); total (47/37) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.50e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 |
TATTGAATTGGCAAGATTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGT > minE/365057‑365183 | tatTGAATTGGCAAGATTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCa > 1:3302608/1‑69 (MQ=255) gAATTGGCAAGATTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACttt < 1:1466419/69‑1 (MQ=255) gAATTGGCAAGATTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACttt < 1:3394292/69‑1 (MQ=255) gAATTGGCAAGATTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACttt < 1:3120798/69‑1 (MQ=255) aTTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCt > 1:2235634/1‑69 (MQ=255) aTTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCt > 1:86094/1‑69 (MQ=255) aTTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCt > 1:2623761/1‑69 (MQ=255) tttttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTg > 1:2637766/1‑69 (MQ=255) ccGGATAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCa < 1:2160782/68‑1 (MQ=255) ccGGATAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCa < 1:1434323/68‑1 (MQ=255) cGGATAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGc > 1:2827592/1‑66 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCATGAACTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGa < 1:967423/69‑1 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCATGAACTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGa < 1:2325290/69‑1 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCATGAACTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGa < 1:2666137/69‑1 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCATGAACTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGa < 1:2863787/69‑1 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCATGAACTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGa < 1:3381361/69‑1 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCATGAACTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGa < 1:927047/69‑1 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCATGAACTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGa < 1:740186/69‑1 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCATGAACTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGa < 1:1502301/69‑1 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCATGAACTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGa < 1:1529655/69‑1 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCATGAACTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGa < 1:1584568/69‑1 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCATGAACTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGa < 1:3368534/69‑1 (MQ=255) tAAGGCGTTCACGCCGCATCCGGCATGAACTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGa < 1:1029349/68‑1 (MQ=255) gCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTg < 1:78020/68‑1 (MQ=255) gCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTg < 1:2604239/68‑1 (MQ=255) gCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTg < 1:823174/68‑1 (MQ=255) cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt > 1:3034848/1‑69 (MQ=255) cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt > 1:2855850/1‑69 (MQ=255) cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt > 1:409340/1‑69 (MQ=255) cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt > 1:3171043/1‑69 (MQ=255) cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt > 1:2634055/1‑69 (MQ=255) cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt > 1:2591460/1‑69 (MQ=255) cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt > 1:2396832/1‑69 (MQ=255) cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt > 1:2542890/1‑69 (MQ=255) cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt > 1:1661740/1‑69 (MQ=255) cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt > 1:1718799/1‑69 (MQ=255) cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt > 1:1942381/1‑69 (MQ=255) cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt > 1:2092800/1‑69 (MQ=255) cATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTg < 1:207253/56‑1 (MQ=255) aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:2084405/69‑1 (MQ=255) aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:1756462/69‑1 (MQ=255) aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:2323848/69‑1 (MQ=255) aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:1507019/69‑1 (MQ=255) aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:2976494/69‑1 (MQ=255) aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:1490904/69‑1 (MQ=255) aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:2783450/69‑1 (MQ=255) aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:204085/69‑1 (MQ=255) aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:263629/69‑1 (MQ=255) aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:261508/69‑1 (MQ=255) aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:252310/69‑1 (MQ=255) aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:1167182/69‑1 (MQ=255) aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:2496100/69‑1 (MQ=255) aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:2430136/69‑1 (MQ=255) aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTAGGTGTTTACGCTCa < 1:616472/69‑1 (MQ=255) ccGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCata < 1:3193085/69‑1 (MQ=255) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGCAGTGAGTATTAATGCTCATATACCacgt > 1:1190122/1‑69 (MQ=21) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCAtata > 1:1401212/1‑63 (MQ=21) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:395254/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:114176/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:159115/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:862452/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:57234/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:1256798/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:1260868/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:3095133/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:2281444/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:1881992/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:195893/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:3284071/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:2608967/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:209894/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:2100706/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:2172339/1‑69 (MQ=25) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa > 1:332060/1‑59 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa > 1:3188574/1‑59 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa > 1:1863334/1‑59 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa > 1:536719/1‑59 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgt > 1:2620567/1‑69 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgt > 1:2268017/1‑69 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgt > 1:2417860/1‑69 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgt > 1:800278/1‑69 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgt > 1:1616896/1‑69 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgt > 1:3278883/1‑69 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacg > 1:2517876/1‑68 (MQ=255) | TATTGAATTGGCAAGATTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGT > minE/365057‑365183 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |