Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 365,160 G→A 27.7% T744T (ACC→ACT fepA ← iron‑enterobactin outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE365,1600GA27.7% 104.9 / 37.3 94T744T (ACC→ACTfepAiron‑enterobactin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (30/37);  new base A (18/8);  total (48/46)
Fisher's exact test for biased strand distribution p-value = 3.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01

Only 100 of 106 total aligned reads displayed.

CACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAGGTATACGCCCCGGC  >  minE/365097‑365216
                                                               |                                                        
cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt                                                     >  1:3171043/1‑69 (MQ=255)
cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt                                                     >  1:2591460/1‑69 (MQ=255)
cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt                                                     >  1:2542890/1‑69 (MQ=255)
cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt                                                     >  1:2634055/1‑69 (MQ=255)
cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt                                                     >  1:1661740/1‑69 (MQ=255)
cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt                                                     >  1:2855850/1‑69 (MQ=255)
cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt                                                     >  1:1718799/1‑69 (MQ=255)
cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt                                                     >  1:3034848/1‑69 (MQ=255)
cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt                                                     >  1:2396832/1‑69 (MQ=255)
cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt                                                     >  1:409340/1‑69 (MQ=255)
cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt                                                     >  1:2092800/1‑69 (MQ=255)
        aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa                                             <  1:1756462/69‑1 (MQ=255)
        aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa                                             <  1:1507019/69‑1 (MQ=255)
        aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa                                             <  1:2496100/69‑1 (MQ=255)
        aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa                                             <  1:2430136/69‑1 (MQ=255)
        aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa                                             <  1:2323848/69‑1 (MQ=255)
        aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa                                             <  1:252310/69‑1 (MQ=255)
        aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa                                             <  1:2084405/69‑1 (MQ=255)
        aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa                                             <  1:204085/69‑1 (MQ=255)
        aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa                                             <  1:261508/69‑1 (MQ=255)
        aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa                                             <  1:263629/69‑1 (MQ=255)
        aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa                                             <  1:2976494/69‑1 (MQ=255)
        aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa                                             <  1:1167182/69‑1 (MQ=255)
        aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTAGGTGTTTACGCTCa                                             <  1:616472/69‑1 (MQ=255)
          ccGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCata                                           <  1:3193085/69‑1 (MQ=255)
                  aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGCAGTGAGTATTAATGCTCATATACCacgt                                   >  1:1190122/1‑69 (MQ=21)
                  aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCAtata                                         >  1:1401212/1‑63 (MQ=21)
                  aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt                                   >  1:862452/1‑69 (MQ=25)
                  aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt                                   >  1:2608967/1‑69 (MQ=25)
                  aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt                                   >  1:209894/1‑69 (MQ=25)
                  aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt                                   >  1:2100706/1‑69 (MQ=25)
                  aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt                                   >  1:2172339/1‑69 (MQ=25)
                  aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt                                   >  1:2281444/1‑69 (MQ=25)
                  aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt                                   >  1:57234/1‑69 (MQ=25)
                  aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt                                   >  1:3284071/1‑69 (MQ=25)
                  aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt                                   >  1:3095133/1‑69 (MQ=25)
                  aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt                                   >  1:114176/1‑69 (MQ=25)
                  aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt                                   >  1:159115/1‑69 (MQ=25)
                  aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt                                   >  1:1260868/1‑69 (MQ=25)
                  aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt                                   >  1:1256798/1‑69 (MQ=25)
                  aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt                                   >  1:195893/1‑69 (MQ=25)
                  aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt                                   >  1:1881992/1‑69 (MQ=25)
                  aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa                                             >  1:3188574/1‑59 (MQ=255)
                  aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa                                             >  1:536719/1‑59 (MQ=255)
                  aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa                                             >  1:332060/1‑59 (MQ=255)
                  aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa                                             >  1:1863334/1‑59 (MQ=255)
                  aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgt                                   >  1:2620567/1‑69 (MQ=255)
                  aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgt                                   >  1:1616896/1‑69 (MQ=255)
                  aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgt                                   >  1:2417860/1‑69 (MQ=255)
                  aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgt                                   >  1:3278883/1‑69 (MQ=255)
                  aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgt                                   >  1:2268017/1‑69 (MQ=255)
                  aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgt                                   >  1:800278/1‑69 (MQ=255)
                  aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacg                                    >  1:2517876/1‑68 (MQ=255)
                        gcgcACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTcc                             >  1:2136116/1‑69 (MQ=255)
                        gcgcACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTcc                             >  1:1173313/1‑69 (MQ=255)
                        gcgcACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTcc                             >  1:1749554/1‑69 (MQ=255)
                        gcgcACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTcc                             >  1:11437/1‑69 (MQ=255)
                           cACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGTGTGTTTACGCTCATATACCACGTACGTCCCgg                          <  1:900934/69‑1 (MQ=255)
                           cACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCgg                          <  1:1236411/69‑1 (MQ=255)
                           cACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCgg                          <  1:1258097/69‑1 (MQ=255)
                           cACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCgg                          <  1:894142/69‑1 (MQ=255)
                           cACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCgg                          <  1:2875507/69‑1 (MQ=255)
                           cACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCgg                          <  1:1392747/69‑1 (MQ=255)
                           cACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCgg                          <  1:1584762/69‑1 (MQ=255)
                            aCTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa                                             <  1:892423/49‑1 (MQ=255)
                                  tCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCACGTACGTCCCGGCTCGtt                    <  1:2083893/68‑1 (MQ=255)
                                  tCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCACGTACGTCCCGGCTCGtt                    <  1:1401423/68‑1 (MQ=255)
                                  tCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCACGTACGTCCCGGCTCGtt                    <  1:882900/68‑1 (MQ=255)
                                  tCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCACGTACGTCCCGGCTCGtt                    <  1:809667/68‑1 (MQ=255)
                                  tCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCACGTACGTCCCGGCTCGtt                    <  1:1886658/68‑1 (MQ=255)
                                  tCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCACGTACGTCCCGGCTCGtt                    <  1:762665/68‑1 (MQ=255)
                                  tCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCACGTACGTCCCGGCTCGtt                    <  1:68819/68‑1 (MQ=255)
                                  tCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCACGTACGTCCCGGCTCGtt                    <  1:1859740/68‑1 (MQ=255)
                                  tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:1181239/67‑1 (MQ=255)
                                  tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:1930080/67‑1 (MQ=255)
                                  tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:1195690/67‑1 (MQ=255)
                                  tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:2402551/67‑1 (MQ=255)
                                  tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:73610/67‑1 (MQ=255)
                                  tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:3051948/67‑1 (MQ=255)
                                  tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:1296697/67‑1 (MQ=255)
                                  tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:2935291/67‑1 (MQ=255)
                                  tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:1978392/67‑1 (MQ=255)
                                  tCAACAATCTGACGTTAGCATCAGAAGTGGGTCTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:3409283/67‑1 (MQ=255)
                                    aacaaTCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTtat                  <  1:691065/68‑1 (MQ=255)
                                    aacaaTCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTtat                  <  1:1483408/68‑1 (MQ=255)
                                      caaTCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAgg               >  1:2801040/1‑69 (MQ=255)
                                      caaTCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAgg               >  1:1500183/1‑69 (MQ=255)
                                         tCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa                                             <  1:1611466/36‑1 (MQ=255)
                                                               ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:1911197/38‑1 (MQ=255)
                                                               ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:3343355/38‑1 (MQ=255)
                                                               ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:2090025/38‑1 (MQ=255)
                                                               ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:678745/38‑1 (MQ=255)
                                                               ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:1867394/38‑1 (MQ=255)
                                                               ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:2499216/38‑1 (MQ=255)
                                                               ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:2567703/38‑1 (MQ=255)
                                                               ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:2910499/38‑1 (MQ=255)
                                                               ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:2027965/38‑1 (MQ=255)
                                                               ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:1017309/38‑1 (MQ=255)
                                                               ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:2732662/38‑1 (MQ=255)
                                                               ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt                     <  1:2050489/38‑1 (MQ=255)
                                                               |                                                        
CACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAGGTATACGCCCCGGC  >  minE/365097‑365216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: