| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 365,168 | C→T | 26.4% | V742I (GTA→ATA) ‡ | fepA ← | iron‑enterobactin outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 365,168 | 0 | C | T | 26.4% | 116.9 / 36.9 | 98 | V742I (GTA→ATA) ‡ | fepA | iron‑enterobactin outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base C (22/50); new base T (18/8); total (40/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.45e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-01 | |||||||||||
ATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAGGTATACGCCCCGGCACCGGCGATATAGTTGGC > minE/365105‑365234 | aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:204085/69‑1 (MQ=255)aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:1490904/69‑1 (MQ=255)aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:2323848/69‑1 (MQ=255)aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:1507019/69‑1 (MQ=255)aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:2084405/69‑1 (MQ=255)aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:2430136/69‑1 (MQ=255)aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:2496100/69‑1 (MQ=255)aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:252310/69‑1 (MQ=255)aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:1756462/69‑1 (MQ=255)aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:261508/69‑1 (MQ=255)aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:263629/69‑1 (MQ=255)aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:2783450/69‑1 (MQ=255)aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:1167182/69‑1 (MQ=255)aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:2976494/69‑1 (MQ=255)aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTAGGTGTTTACGCTCa < 1:616472/69‑1 (MQ=255) ccGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCata < 1:3193085/69‑1 (MQ=255) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGCAGTGAGTATTAATGCTCATATACCacgt > 1:1190122/1‑69 (MQ=21) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCAtata > 1:1401212/1‑63 (MQ=21) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:2281444/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:395254/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:2172339/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:2100706/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:209894/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:114176/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:57234/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:3284071/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:2608967/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:3095133/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:195893/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:1881992/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:1256798/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:1260868/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:862452/1‑69 (MQ=25) aaCTACGCGCACTTTGTCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCacgt > 1:159115/1‑69 (MQ=25) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa > 1:1863334/1‑59 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa > 1:332060/1‑59 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa > 1:3188574/1‑59 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa > 1:536719/1‑59 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgt > 1:1616896/1‑69 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgt > 1:3278883/1‑69 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgt > 1:800278/1‑69 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgt > 1:2268017/1‑69 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgt > 1:2620567/1‑69 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgt > 1:2417860/1‑69 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacg > 1:2517876/1‑68 (MQ=255) gcgcACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTcc > 1:1749554/1‑69 (MQ=255) gcgcACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTcc > 1:2136116/1‑69 (MQ=255) gcgcACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTcc > 1:1173313/1‑69 (MQ=255) gcgcACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTcc > 1:2325093/1‑69 (MQ=255) gcgcACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTcc > 1:11437/1‑69 (MQ=255) cACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGTGTGTTTACGCTCATATACCACGTACGTCCCgg < 1:900934/69‑1 (MQ=255) cACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCgg < 1:1258097/69‑1 (MQ=255) cACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCgg < 1:2875507/69‑1 (MQ=255) cACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCgg < 1:1392747/69‑1 (MQ=255) cACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCgg < 1:1236411/69‑1 (MQ=255) cACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCgg < 1:1584762/69‑1 (MQ=255) cACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCgg < 1:894142/69‑1 (MQ=255) aCTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:892423/49‑1 (MQ=255) tCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCACGTACGTCCCGGCTCGtt < 1:1886658/68‑1 (MQ=255) tCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCACGTACGTCCCGGCTCGtt < 1:1401423/68‑1 (MQ=255) tCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCACGTACGTCCCGGCTCGtt < 1:882900/68‑1 (MQ=255) tCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCACGTACGTCCCGGCTCGtt < 1:809667/68‑1 (MQ=255) tCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCACGTACGTCCCGGCTCGtt < 1:2083893/68‑1 (MQ=255) tCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCACGTACGTCCCGGCTCGtt < 1:1859740/68‑1 (MQ=255) tCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCACGTACGTCCCGGCTCGtt < 1:68819/68‑1 (MQ=255) tCAACAATCTGACGTTAGCTTCAGAAGTGAGTATTAATGCTCATATACCACGTACGTCCCGGCTCGtt < 1:762665/68‑1 (MQ=255) tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:73610/67‑1 (MQ=255) tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:3051948/67‑1 (MQ=255) tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:2402551/67‑1 (MQ=255) tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:2935291/67‑1 (MQ=255) tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:1181239/67‑1 (MQ=255) tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:1195690/67‑1 (MQ=255) tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:1296697/67‑1 (MQ=255) tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:1930080/67‑1 (MQ=255) tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:1978392/67‑1 (MQ=255) tCAACAATCTGACGTTAGCATCAGAAGTGGGTCTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:3409283/67‑1 (MQ=255) aacaaTCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTtat < 1:1483408/68‑1 (MQ=255) aacaaTCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTtat < 1:691065/68‑1 (MQ=255) caaTCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAgg > 1:2801040/1‑69 (MQ=255) caaTCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAgg > 1:1500183/1‑69 (MQ=255) tCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:1611466/36‑1 (MQ=255) cATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAGGTATACGCCCCGGc < 1:969651/68‑1 (MQ=255) ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:2090025/38‑1 (MQ=255) ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:2050489/38‑1 (MQ=255) ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:2027965/38‑1 (MQ=255) ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:678745/38‑1 (MQ=255) ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:1017309/38‑1 (MQ=255) ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:3343355/38‑1 (MQ=255) ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:1911197/38‑1 (MQ=255) ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:1867394/38‑1 (MQ=255) ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:2499216/38‑1 (MQ=255) ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:2567703/38‑1 (MQ=255) ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:2732662/38‑1 (MQ=255) ggTGTTTACGCTCATATACCACGTACGTCCCGGCTCGt < 1:2910499/38‑1 (MQ=255) gtTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAGGTATACGCCCCGGCACCGGCGATATAGtt > 1:362947/1‑69 (MQ=255) gtTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAGGTATACGCCCCGGCACCGGCGATATAGtt > 1:3283090/1‑69 (MQ=255) gtTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAGGTATACGCCCCGGCACCGGCGATATAGtt > 1:2588711/1‑69 (MQ=255) tACGCTCATATACCACGTACGTCCCGGCTCGTTATAGGTATACGCCCCGGCACCGGCGATATAGTTGGc > 1:2974628/1‑69 (MQ=255) | ATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAGGTATACGCCCCGGCACCGGCGATATAGTTGGC > minE/365105‑365234 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |