| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 365,751 | G→C | 28.3% | V547V (GTC→GTG) | fepA ← | iron‑enterobactin outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 365,751 | 0 | G | C | 28.3% | 78.3 / 35.1 | 60 | V547V (GTC→GTG) | fepA | iron‑enterobactin outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base G (22/21); new base C (6/11); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.22e-01 | |||||||||||
TGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTCCAGACCAATCTCTTTGTT > minE/365685‑365801 | tGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGAc > 1:3270949/1‑69 (MQ=255) cAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCcccc > 1:3126556/1‑69 (MQ=255) cAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCAcc > 1:2283130/1‑69 (MQ=255) cAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCAcc > 1:2493196/1‑69 (MQ=255) gCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCg > 1:2915335/1‑69 (MQ=255) cacaTAGCCCGCTTCTATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTc > 1:1533982/1‑69 (MQ=255) cacaTAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTc > 1:2741633/1‑69 (MQ=255) cacaTAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTc > 1:13988/1‑69 (MQ=255) cacaTAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAACCGTc < 1:200213/69‑1 (MQ=255) cacaTAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAACCGTc < 1:2939301/69‑1 (MQ=255) cacaTAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAACCGTc < 1:158100/69‑1 (MQ=255) cacaTAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAACCGTc < 1:3282905/69‑1 (MQ=255) cacaTAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAACCGTc < 1:3304412/69‑1 (MQ=255) cacaTAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAACCGTc < 1:3345597/69‑1 (MQ=255) cacaTAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAACCGTc < 1:3349459/69‑1 (MQ=255) cacaTAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAACCGTc < 1:399799/69‑1 (MQ=255) cacaTAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAACCGTc < 1:485492/69‑1 (MQ=255) cacaTAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAACCGTc < 1:1050299/69‑1 (MQ=255) cacaTAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGAGACGCCCGCCAGCCACCCCTc > 1:2739056/1‑69 (MQ=255) acaTAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAACCGTc < 1:1652038/68‑1 (MQ=255) tAGCCCGCTTCGATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGt > 1:2521273/1‑69 (MQ=255) tAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTcgcg > 1:2745331/1‑68 (MQ=255) tAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGt > 1:842885/1‑69 (MQ=255) tAGCCCGCTTCAATCTTATTGCGATAATCGTTAAGGAACCAGGTGACGCCCGCCGGCCACCCGTcgcg > 1:1751474/1‑68 (MQ=255) tAGCCCGCATCAATCTTATTGCGATAATCGTTACGGAAGCAGGTGAc > 1:2318634/1‑47 (MQ=255) ccGCTTCAATCTTATTGCGATAATCGTTACTGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTg < 1:2220963/68‑1 (MQ=255) ccGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTg < 1:612359/68‑1 (MQ=255) ccGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTg < 1:2640827/68‑1 (MQ=255) ccGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTg < 1:1152778/68‑1 (MQ=255) ttCAATCTTATTGGGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTc < 1:1221550/69‑1 (MQ=255) ttCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTc < 1:2303197/69‑1 (MQ=255) ttCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTc < 1:373550/69‑1 (MQ=255) ttCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTc < 1:3271339/69‑1 (MQ=255) ttCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTc < 1:1910877/69‑1 (MQ=255) ttCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTc < 1:325821/69‑1 (MQ=255) ttCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTc < 1:2705978/69‑1 (MQ=255) tCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTc < 1:1676248/68‑1 (MQ=255) aaTCTTATTGCGATAATCGTTACGGCACCAGGTCAcgcccgc > 1:2957554/1‑42 (MQ=38) aaTCTTATTGCGATAATCGTTACGGAACCAGGTCAcgcccgc > 1:1994228/1‑42 (MQ=255) aaTCTTATTGCGATAATCGTTACGGAACCAGGTCAcgcccgc > 1:388663/1‑42 (MQ=255) aaTCTTATTGCGATAATCGTTACGGAACCAGGTCAcgcccgc > 1:187827/1‑42 (MQ=255) aaTCTTATTGCGATAATCGTTACGGAACCAGGTCAcgcccgc > 1:2707086/1‑42 (MQ=255) aaTCTTATTGCGATAATCGTTACGGAACCAGGTCAcgc > 1:1692232/1‑38 (MQ=255) aTTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTg > 1:2009024/1‑55 (MQ=255) aTTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTg > 1:2426930/1‑55 (MQ=255) aTTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTCCAGACCaa > 1:1077074/1‑68 (MQ=255) aTTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTCCAGACCaa > 1:2462815/1‑68 (MQ=255) aTTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTCCAGACCaa > 1:2780260/1‑68 (MQ=255) gCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTcgct < 1:480618/48‑2 (MQ=255) gCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTcgcg < 1:2954902/48‑1 (MQ=255) gCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTcgcg < 1:2641692/48‑1 (MQ=255) gCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTcgcg < 1:194159/48‑1 (MQ=255) gCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTcgcg < 1:1282123/48‑1 (MQ=255) gATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTc < 1:1429531/55‑1 (MQ=255) aTCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTCCAGATCAATCTCTttgtt < 1:1346338/69‑1 (MQ=255) aTCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTCCAGACCaa > 1:2559137/1‑59 (MQ=255) aTCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTCCAGACCAAt > 1:1072300/1‑60 (MQ=255) aTCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTCCAGACCAAt > 1:237861/1‑60 (MQ=255) aTCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTCCAGACCAATCTCTttgtt < 1:1304709/69‑1 (MQ=255) tCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTCCAGACCAATCTCTttgtt < 1:536229/68‑1 (MQ=255) gACGCCCGCCAGCCACCCGTCGCGTTTGAACTCCAGACCaa > 1:1615818/1‑41 (MQ=255) gACGCCCGCCAGCCACCCGTCGCGTTTGAACTCCAGACCAATCTCTttgtt > 1:145905/1‑51 (MQ=255) gACGCCCGCCAGCCACCCGTCGCGTTTGAACTCCAGACCAATCTCTttgtt < 1:29644/51‑1 (MQ=255) gACGCCCGCCAGCCACCCGTCGCGTTTGAACTCCAGACCAATCTCTttgtt < 1:52527/51‑1 (MQ=255) gACGCCCGCCAGCCACCCGTCGCGTTTGAACTCCAGACCAATCTCTttgtt > 1:2626558/1‑51 (MQ=255) | TGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTCCAGACCAATCTCTTTGTT > minE/365685‑365801 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |