| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 368,298 | A→G | 47.1% | Q196R (CAG→CGG) | fes → | enterobactin/ferric enterobactin esterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 368,298 | 0 | A | G | 47.1% | 4.0 / 74.1 | 85 | Q196R (CAG→CGG) | fes | enterobactin/ferric enterobactin esterase |
| Reads supporting (aligned to +/- strand): ref base A (10/35); new base G (24/16); total (34/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.57e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
CGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAAC > minE/368233‑368363 | cGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCa < 1:2534407/69‑1 (MQ=255)cGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCa < 1:2388570/69‑1 (MQ=255)cGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCa < 1:1515564/69‑1 (MQ=255)cGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCa < 1:1627810/69‑1 (MQ=255) gATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCa < 1:1319481/68‑1 (MQ=255) gAATTTTGGGCGCAGAGTATGCCCGTCTGGCCTGCGCTGACTTCGCTGACCCATCGTCGGCAACTtccg > 1:2832946/1‑68 (MQ=255) gAATTTTGGGCGCAGAGTATGCCCGTCTGGCCTGCGCTGACTTCGCTGACCCATCGTCGGCAACTtccg > 1:601425/1‑68 (MQ=255) gAATTTTGGGCGCAGAGTATGCCCGTCTGGCCTGCGCTGACTTCGCTGACCCATCGTCGGCAACTtccg > 1:2610701/1‑68 (MQ=255) gAATTTTGGGCGCAGAGTATGCCCGTCTGGCCTGCGCTGACTTCGCTGACCCATCGTCGGCAACTtccg > 1:2668660/1‑68 (MQ=255) gAATTTTGGGCGCAGAGTATGCCCGTCTGGCCTGCGCTGACTTCGCTGACCCATCGTCGGCAACTtccg > 1:774876/1‑68 (MQ=255) gAATTTTGGGCGCAGAGTATGCCCGTCTGGCCTGCGCTGACTTCGCTGACCCATCGTCGGCAACTtccg > 1:59905/1‑68 (MQ=255) gAATTTTGGGCGCAGAGTATGCCCGTCTGGCCTGCGCTGACTTCGCTGACCCATCGTCGGCAACTtccg > 1:542407/1‑68 (MQ=255) gAATTTTGGGCGCAGAGTATGCCCGTCTGGCCTGCGCTGACTTCGCTGACCCATCGTCGGCAACTtccg > 1:2721580/1‑68 (MQ=255) gAATTTTGGGCGCAGAGTATGCCCGTCTGGCCTGCGCTGACTTCGCTGACCCATCGTCGGCAACTtccg > 1:2960252/1‑68 (MQ=255) gAATTTTGGGCGCAGAGTATGCCCGTCTGGCCTGCGCTGACTTCGCTGACCCATCGTCGGCAACTtccg > 1:1713084/1‑68 (MQ=255) gAATTTTGGGCGCAGAGTATGCCCGTCTGGCCTGCGCTGACTTCGCTGACCCATCGTCGGCAACTtccg > 1:1649051/1‑68 (MQ=255) gAATTTTGGGCGCAGAGTATGCCCGTCTGGCCTGCGCTGACTTCGCTGACCCATCGTCGGCAACTtccg > 1:1339338/1‑68 (MQ=255) gAATTTTGGGCGCAGAGTATGCCCGTCTGGCCTGCGCTGACTTCGCTGACCCATCGTCGGCAACTtccg > 1:967674/1‑68 (MQ=255) cgcAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCgtg < 1:2121258/68‑1 (MQ=255) gcAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTa < 1:52024/69‑1 (MQ=255) gcAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTa < 1:2748389/69‑1 (MQ=255) gcAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTa < 1:840204/69‑1 (MQ=255) gcAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTa < 1:3273989/69‑1 (MQ=255) aTGCCCGTCTTGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTg < 1:2099350/69‑1 (MQ=255) aTGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTg < 1:250146/69‑1 (MQ=255) aTGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTg < 1:2695411/69‑1 (MQ=255) aTGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTg < 1:2972953/69‑1 (MQ=255) aTGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTg < 1:3240526/69‑1 (MQ=255) aTGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTg < 1:342638/69‑1 (MQ=255) aTGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTg < 1:3354972/69‑1 (MQ=255) aTGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTg < 1:2443152/69‑1 (MQ=255) aTGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTg < 1:2375731/69‑1 (MQ=255) aTGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTg < 1:2313638/69‑1 (MQ=255) aTGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTg < 1:2183376/69‑1 (MQ=255) aTGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTg < 1:20400/69‑1 (MQ=255) tGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGa > 1:1195527/1‑69 (MQ=255) tGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGa > 1:3404640/1‑69 (MQ=255) tGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGa > 1:599304/1‑69 (MQ=255) tGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCt < 1:668986/69‑1 (MQ=255) tGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCt < 1:133834/69‑1 (MQ=255) ggCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCt < 1:2528138/68‑1 (MQ=255) cAGTGCTGACTTCGCTGAGCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCTATc < 1:1210909/68‑1 (MQ=255) cAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCTATc < 1:248475/68‑1 (MQ=255) cAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCTATc < 1:3044544/68‑1 (MQ=255) cAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCTATc < 1:1816131/68‑1 (MQ=255) cAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCTATc < 1:2261867/68‑1 (MQ=255) gCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCTATCGacac < 1:2066387/69‑1 (MQ=255) gCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACCCTATCGacac < 1:2055963/69‑1 (MQ=255) gccccATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACcgcg > 1:1298579/3‑68 (MQ=255) gACCCATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATc > 1:2601069/1‑43 (MQ=38) gACCCATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACcgcg > 1:2739987/1‑68 (MQ=255) gACCCATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACcgcg > 1:2637229/1‑68 (MQ=255) gACCCATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACcgcg > 1:3253542/1‑68 (MQ=255) gACCCATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACcgcg > 1:3069608/1‑68 (MQ=255) gACCCATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACcgcg > 1:2971765/1‑68 (MQ=255) gACCCATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACcgcg > 1:2774855/1‑68 (MQ=255) gACCCATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACcgcg > 1:206325/1‑68 (MQ=255) gACCCATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACcgcg > 1:538785/1‑68 (MQ=255) gACCCATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACcgc > 1:784986/1‑67 (MQ=255) ccATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGccc < 1:845311/68‑1 (MQ=255) ccATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGccc < 1:485121/68‑1 (MQ=255) ccATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGccc < 1:833075/68‑1 (MQ=255) ccATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGccc < 1:417460/68‑1 (MQ=255) ccATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGccc < 1:2693437/68‑1 (MQ=255) ccATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGccc < 1:3309335/68‑1 (MQ=255) ccATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGccc < 1:1311128/68‑1 (MQ=255) ccATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGccc < 1:1491254/68‑1 (MQ=255) ccATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGccc < 1:2181869/68‑1 (MQ=255) ccATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGccc < 1:2603216/68‑1 (MQ=255) ccATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGccc < 1:2650567/68‑1 (MQ=255) ccATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGccc < 1:2675791/68‑1 (MQ=255) ccATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGccc < 1:1085036/68‑1 (MQ=255) ccATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGccc < 1:2730244/68‑1 (MQ=255) ccATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGccc < 1:3248105/68‑1 (MQ=255) ccATCGTCGGCAACTTCCGCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGccc < 1:3313382/68‑1 (MQ=255) tCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGa < 1:592988/69‑1 (MQ=255) tCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGa < 1:1970775/69‑1 (MQ=255) tCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGa < 1:1230733/69‑1 (MQ=255) gTCAGCAACTTCCTCCCGCCGTGTATGTGTTGGTCGACGCTATCGACACCACGCCCCGCGCCCCCGAAc > 1:2431871/1‑69 (MQ=255) gTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGaa > 1:535894/1‑68 (MQ=255) gTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAAc > 1:2753359/1‑69 (MQ=255) gTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAAc > 1:3266201/1‑69 (MQ=255) gTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAAc > 1:3431746/1‑69 (MQ=255) gTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAAc > 1:980503/1‑69 (MQ=255) gTCAGCAACTTCCTACCGCCGTGTATGTGTTGATCGACGc > 1:2201133/1‑40 (MQ=255) | CGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAAC > minE/368233‑368363 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |