| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 41,783 | G→A | 29.8% | A306A (GCG→GCA) | fixB → | predicted electron transfer flavoprotein, NAD/FAD‑binding domain and ETFP adenine nucleotide‑binding domain‑like |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 41,783 | 0 | G | A | 29.8% | 86.5 / 38.4 | 87 | A306A (GCG→GCA) | fixB | predicted electron transfer flavoprotein, NAD/FAD‑binding domain and ETFP adenine nucleotide‑binding domain‑like |
| Reads supporting (aligned to +/- strand): ref base G (44/17); new base A (20/6); total (64/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01 | |||||||||||
AATGCGCCGATCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAGGGCTGCATTTTGGCCCTGCCGCTGA > minE/41718‑41845 | aaTGCGCCGATCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTg > 1:3383659/1‑69 (MQ=255)aaTGCGCCGATCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTg > 1:68711/1‑69 (MQ=255)aaTGCGCCGATCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTg > 1:1781012/1‑69 (MQ=255)aaTGCGCCGATCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTg > 1:948441/1‑69 (MQ=255)aaTGCGCCGATCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTg > 1:1366517/1‑69 (MQ=255)aaTGCGCCGATCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTg > 1:1342692/1‑69 (MQ=255)aaTGCGCCGATCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTg > 1:3277789/1‑69 (MQ=255)aaTGCGCCGATCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTg > 1:309959/1‑69 (MQ=255)aaTGCGCCGATCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATACTTCCGGCGCTg > 1:1970532/1‑69 (MQ=255)aaTGCGCCGATCTTCCAGTAAGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCGGGCGCTg > 1:3368097/1‑69 (MQ=255) cTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCGGCttt > 1:2065295/1‑69 (MQ=255) cTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGc > 1:1994262/1‑66 (MQ=255) cTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCttt > 1:97628/1‑69 (MQ=255) cTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCttt > 1:2817911/1‑69 (MQ=255) cTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCttt > 1:3178346/1‑69 (MQ=255) cTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCttt > 1:2089086/1‑69 (MQ=255) cTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGAAGCttt > 1:2980801/1‑69 (MQ=255) cTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTACCGGCGCTGACCGCAGCttt > 1:559659/1‑69 (MQ=255) cgcgGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTg > 1:2541926/1‑69 (MQ=255) cgcgGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTg > 1:633605/1‑69 (MQ=255) cgcgGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTg > 1:2229156/1‑69 (MQ=255) cgcgGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTg > 1:617965/1‑69 (MQ=255) cgcgGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTg > 1:26335/1‑69 (MQ=255) cgcgGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTg > 1:2667937/1‑69 (MQ=255) cgcgGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTg > 1:2815889/1‑69 (MQ=255) cgcgGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTg > 1:1032300/1‑69 (MQ=255) cgcgGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTg > 1:1847353/1‑69 (MQ=255) cgcgGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTg > 1:155909/1‑69 (MQ=255) ggATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATc < 1:2630492/69‑1 (MQ=255) ggATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATc < 1:3076643/69‑1 (MQ=255) ggATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATc < 1:284968/69‑1 (MQ=255) ggATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATc < 1:240733/69‑1 (MQ=255) ggATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCTCAGCTTTAGCGCGTTGAt < 1:1597436/68‑1 (MQ=255) ggATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGAt < 1:2640897/68‑1 (MQ=255) ggATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGAt < 1:2806198/68‑1 (MQ=255) ggATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGAt < 1:2818955/68‑1 (MQ=255) ggATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGAt < 1:3384603/68‑1 (MQ=255) ggATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGAt < 1:589713/68‑1 (MQ=255) ttACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCAc < 1:3369831/69‑1 (MQ=255) ttACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCAc < 1:1328052/69‑1 (MQ=255) ttACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCAc < 1:2180474/69‑1 (MQ=255) ttACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCAc < 1:2005427/69‑1 (MQ=255) aCGGCATTGTTGGCGCCGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGATCCActc > 1:790267/1‑69 (MQ=255) aCGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGtt > 1:886644/1‑60 (MQ=255) aCGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGATCCActc > 1:2931326/1‑69 (MQ=255) aCGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGATCCActc > 1:1227182/1‑69 (MQ=255) aCGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGATCCActc > 1:1300206/1‑69 (MQ=255) aCGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGATCCActc > 1:1201578/1‑69 (MQ=255) aCGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGATCCActc > 1:1165684/1‑69 (MQ=255) aCGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGATCCActc > 1:1089514/1‑69 (MQ=255) aCGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGATCCActc > 1:259421/1‑69 (MQ=255) aCGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGATCCActc > 1:2791091/1‑69 (MQ=255) aCGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGATCCActc > 1:1368325/1‑69 (MQ=255) aCGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGATCCActc > 1:13883/1‑69 (MQ=255) aCGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGATCCActc > 1:2540740/1‑69 (MQ=255) aCGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGATCCActc > 1:410030/1‑69 (MQ=255) aCGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGATCCActc > 1:901882/1‑69 (MQ=255) aCGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGATCCActc > 1:876434/1‑69 (MQ=255) aCGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGATCCActc > 1:1807144/1‑69 (MQ=255) aCGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGATCCActc > 1:1976475/1‑69 (MQ=255) aCGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGATCCAct > 1:2569140/1‑68 (MQ=255) aCGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCACTGACCGCAGCTTTAGCGCGTTGATCCAct > 1:3072726/1‑68 (MQ=255) gttgGCGACGCCGTGAAGATCCTTCTGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAggg > 1:980984/1‑69 (MQ=255) gttgGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAggg > 1:987577/1‑69 (MQ=255) gttgGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAggg > 1:685396/1‑69 (MQ=255) gttgGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAggg > 1:329421/1‑69 (MQ=255) gttgGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAggg > 1:3168147/1‑69 (MQ=255) gttgGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAggg > 1:3187465/1‑69 (MQ=255) gttgGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAggg > 1:1251705/1‑69 (MQ=255) gttgGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAggg > 1:1420349/1‑69 (MQ=255) gttgGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAggg > 1:2252810/1‑69 (MQ=255) gttgGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAggg > 1:2012346/1‑69 (MQ=255) gttgGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAggg > 1:1650989/1‑69 (MQ=255) gttgGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAggg > 1:1657647/1‑69 (MQ=255) gttgGCGACGCCGTGAAGATCATTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAggg > 1:2802266/1‑69 (MQ=255) ccTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAGGGCTGCATTTTGGCCCTGcc > 1:1420824/1‑67 (MQ=255) ccTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAGGGCTGCATTTTGGCCCTGCCg > 1:2557186/1‑68 (MQ=255) ccTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAGGGCTGCATTTTGGCCCTGCCg > 1:3413987/1‑68 (MQ=255) cTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAGGGCTGCATTTTGGCCCTGCCGCt < 1:1062446/69‑1 (MQ=255) cTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAGGGCTGCATTTTGGCCCTGCCGCt < 1:1624125/69‑1 (MQ=255) cTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAGGGCTGCATTTTGGCCCTGCCGCt < 1:1881242/69‑1 (MQ=255) cTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAGGGCTGCATTTTGGCCCTGCCGCt < 1:2099761/69‑1 (MQ=255) cTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAGGGCTGCATTTTGGCCCTGCCGCt < 1:2160103/69‑1 (MQ=255) cTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAGGGCTGCATTTTGGCCCTGCCGCt < 1:239615/69‑1 (MQ=255) cTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAGGGCTGCATTTTGGCCCTGCCGCt < 1:2582700/69‑1 (MQ=255) ttCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAGGGCTGCATTTTGGCCCTGCCGCt < 1:2281487/68‑1 (MQ=255) tCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAGGGCTGCATTTTGGCCCTGCCGCTGa < 1:1948745/69‑1 (MQ=255) | AATGCGCCGATCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAGGGCTGCATTTTGGCCCTGCCGCTGA > minE/41718‑41845 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |