| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 370,232 | G→A | 38.3% | E393E (GAG→GAA) | entF → | enterobactin synthase multienzyme complex component, ATP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 370,232 | 0 | G | A | 38.3% | 40.0 / 45.0 | 73 | E393E (GAG→GAA) | entF | enterobactin synthase multienzyme complex component, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (38/7); new base A (23/5); total (61/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
TTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATG > minE/370177‑370298 | ttAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATa < 1:285940/69‑1 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATaaa > 1:1204462/1‑59 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGt > 1:1019910/1‑65 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:3332373/1‑68 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:2717090/1‑68 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:2909629/1‑68 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:2283278/1‑68 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:2197721/1‑68 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:2193751/1‑68 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:2189874/1‑68 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:2934442/1‑68 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:2995588/1‑68 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:1953514/1‑68 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:416198/1‑68 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:1579225/1‑68 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:487565/1‑68 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:543680/1‑68 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:862325/1‑68 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:1060568/1‑68 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:595170/1‑68 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:1522210/1‑68 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:1524177/1‑68 (MQ=255) aaCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:531243/1‑68 (MQ=255) aaCTGGACCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTAc > 1:4530/1‑68 (MQ=255) aCTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTAc < 1:2660115/67‑1 (MQ=255) aCTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTAc < 1:3065079/67‑1 (MQ=255) aCTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTAc < 1:2255740/67‑1 (MQ=255) aCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTACg < 1:1007917/68‑1 (MQ=255) aCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTACg < 1:1817471/68‑1 (MQ=255) aCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTACg < 1:3198189/68‑1 (MQ=255) aCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTACg < 1:2417327/68‑1 (MQ=255) aCTGGCCCTGTTCCCGGATGAACACGGTGATTTGAGTATTGAAATCCTCGCCAATAAACAGCGTTACg < 1:1365916/68‑1 (MQ=255) ggCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATg > 1:1109412/1‑68 (MQ=255) gCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATg > 1:1804682/1‑67 (MQ=255) gCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGa > 1:73429/1‑68 (MQ=255) gCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGa > 1:637007/1‑68 (MQ=255) gCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGa > 1:602488/1‑68 (MQ=255) gCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGa > 1:427149/1‑68 (MQ=255) gCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGa > 1:565487/1‑68 (MQ=255) gCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGa > 1:3244935/1‑68 (MQ=255) gCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGa > 1:262478/1‑68 (MQ=255) gCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGa > 1:2616922/1‑68 (MQ=255) gCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGa > 1:1416495/1‑68 (MQ=255) gCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGa > 1:1668498/1‑68 (MQ=255) gCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGa > 1:2172252/1‑68 (MQ=255) gCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGa > 1:1156995/1‑68 (MQ=255) gCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGa > 1:2492057/1‑68 (MQ=255) gCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGa > 1:2026858/1‑68 (MQ=255) gCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGa > 1:1791278/1‑68 (MQ=255) gCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGa > 1:2437281/1‑68 (MQ=255) gTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATa > 1:156599/1‑47 (MQ=255) gTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATa > 1:3043109/1‑47 (MQ=255) gTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATa > 1:2880753/1‑47 (MQ=255) gTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATa > 1:2788787/1‑47 (MQ=255) gTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATa > 1:1726588/1‑47 (MQ=255) tGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATcc > 1:1797272/1‑68 (MQ=255) tGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATcc > 1:78516/1‑68 (MQ=255) tGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATcc > 1:418560/1‑68 (MQ=255) tGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATcc > 1:2492228/1‑68 (MQ=255) tGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATcc > 1:2940374/1‑68 (MQ=255) tGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATcc > 1:2582137/1‑68 (MQ=255) tGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATcc > 1:2145911/1‑68 (MQ=255) gTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTg < 1:2505991/69‑1 (MQ=255) gTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTg < 1:1045032/69‑1 (MQ=255) tGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTg < 1:3389511/68‑1 (MQ=255) ttGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCACCGTTAATCCAg > 1:508837/1‑50 (MQ=255) ttGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGaa > 1:2499147/1‑67 (MQ=255) ttGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGaa > 1:2975915/1‑67 (MQ=255) ttGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGaa > 1:2629321/1‑67 (MQ=255) ttGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGaa > 1:1135837/1‑67 (MQ=255) ttGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGaa > 1:3259978/1‑67 (MQ=255) ttGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGaa > 1:1533986/1‑67 (MQ=255) ttGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGaa > 1:3426116/1‑67 (MQ=255) gagaTCCTCTCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGc < 1:1573009/60‑1 (MQ=255) gagaTCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGc < 1:2706657/60‑1 (MQ=255) gagaTCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGc < 1:3242526/60‑1 (MQ=255) gagaTCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGc < 1:436726/60‑1 (MQ=255) gagaTCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGc < 1:3014112/60‑1 (MQ=255) gagaTCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAAt > 1:380967/1‑68 (MQ=255) gagaTCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATg > 1:863394/1‑69 (MQ=255) gagaTCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATg > 1:1479182/1‑69 (MQ=255) gagaTCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATg > 1:80316/1‑69 (MQ=255) gagaTCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATg > 1:1416090/1‑69 (MQ=255) gagaTCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATg > 1:489977/1‑69 (MQ=255) gagaTCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATg > 1:3346143/1‑69 (MQ=255) gagaTCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATg > 1:2061851/1‑69 (MQ=255) gagaTCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATg > 1:2159764/1‑69 (MQ=255) gagaTCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATg > 1:2316455/1‑69 (MQ=255) gagaTCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATg > 1:2908322/1‑69 (MQ=255) gagaTCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATg > 1:2862685/1‑69 (MQ=255) gagaTCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATg > 1:2852040/1‑69 (MQ=255) gagaTCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATg > 1:2460236/1‑69 (MQ=255) gagaTCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATg > 1:2548277/1‑69 (MQ=255) | TTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATG > minE/370177‑370298 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |