| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 372,206 | T→C | 53.4% | R1051R (CGT→CGC) | entF → | enterobactin synthase multienzyme complex component, ATP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 372,206 | 0 | T | C | 53.4% | 2.8 / 35.0 | 45 | R1051R (CGT→CGC) | entF | enterobactin synthase multienzyme complex component, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (3/18); new base C (15/9); total (18/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.90e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
ATGGTCGCGTCAACTGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGCCCGACGCTGTTTT > minE/372141‑372261 | aTGGTCGCGTCAACTGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTAt > 1:2230390/1‑68 (MQ=255)aTGGTCGCGTCAACTGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTAt > 1:3239559/1‑68 (MQ=255) tGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTATGGGATTCGAAACCAt < 1:197968/69‑1 (MQ=255) tGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTATGGGATTCGAAACCAt < 1:2309926/69‑1 (MQ=255) tGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTATGGGATTCGAAACCAt < 1:2306106/69‑1 (MQ=255) tGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTATGGGATTCGAAACCAt < 1:281463/69‑1 (MQ=255) tGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTATGGGATTCGAAACCAt < 1:2881767/69‑1 (MQ=255) tGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTATGGGATTCGAAACCAt < 1:3121694/69‑1 (MQ=255) tGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTATGGGATTCGAAACCAt < 1:2067988/69‑1 (MQ=255) tGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTATGGGATTCGAAACCAt < 1:2034153/69‑1 (MQ=255) tGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTATGGGATTCGAAACCAt < 1:1817343/69‑1 (MQ=255) tGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTATGGGATTCGAAACCAt < 1:362101/69‑1 (MQ=255) tGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTATGGGATTCGAAACCAt < 1:570380/69‑1 (MQ=255) tGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTATGGGATTCGAAACCAt < 1:772583/69‑1 (MQ=255) cAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTATGGGATTCGAAACCATTCTGc < 1:1727888/68‑1 (MQ=255) cAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTATGGGATTCGAAACCATTCTGc < 1:388006/68‑1 (MQ=255) aaCTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTATGGGATTCGAAACCATTCTGCCGt < 1:680813/69‑1 (MQ=255) aaCTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTATGGGATTCGAAACCATTCTGCCGt < 1:1010944/69‑1 (MQ=255) aaCTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTATGGGATTCGAAACCATTCTGCCGt < 1:101375/69‑1 (MQ=255) agaagaCAGCACCCAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGt > 1:2308787/1‑55 (MQ=255) agaagaCAGCACCCAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTa > 1:3369180/1‑56 (MQ=255) agaagaCAGCACCCAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTa > 1:946361/1‑56 (MQ=255) agaagaCAGCACCCAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTa > 1:736375/1‑56 (MQ=255) agaagaCAGCACCCAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTa > 1:833993/1‑56 (MQ=255) agaagaCAGCACCCAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTa > 1:2673109/1‑56 (MQ=255) agaagaCAGCACCCAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTa > 1:2663979/1‑56 (MQ=255) agaagaCAGCACCCAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTa > 1:2412051/1‑56 (MQ=255) agaagaCAGCACCCAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTa > 1:2199741/1‑56 (MQ=255) agaagaCAGCACCCAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTa > 1:211849/1‑56 (MQ=255) agaagaCAGCACCCAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTa > 1:2076057/1‑56 (MQ=255) agaagaCAGCACCCAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTa > 1:2015204/1‑56 (MQ=255) agaagaCAGCACCCAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTa > 1:112366/1‑56 (MQ=255) agaagaCAGCACCCAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTa > 1:1534047/1‑56 (MQ=255) agaagaCAGCACCCAGCGCATGGGATTCGAAACCATTCTGCCGATGCGTGAAGGTa > 1:774415/1‑56 (MQ=255) aagaCAGCACCCGGCGTATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGCCCGACGCtgt > 1:139486/1‑69 (MQ=255) aCAGCACCCGGCGTATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGCCCGACGCtgtttt < 1:1034361/69‑1 (MQ=255) cccAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGc < 1:455430/50‑1 (MQ=39) cccAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGc < 1:1609220/50‑1 (MQ=39) cccAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGc < 1:1341762/50‑1 (MQ=39) cccAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGc < 1:732111/50‑1 (MQ=39) cccAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGc < 1:1317809/50‑1 (MQ=39) cccAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGc < 1:1751787/50‑1 (MQ=39) cccAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGc < 1:2265164/50‑1 (MQ=39) cccAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGc < 1:1121821/50‑1 (MQ=39) cccAGCGCATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGc < 1:2285973/50‑1 (MQ=39) | ATGGTCGCGTCAACTGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGCGTATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGCCCGACGCTGTTTT > minE/372141‑372261 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |