| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 372,419 | G→C | 25.2% | T1122T (ACG→ACC) | entF → | enterobactin synthase multienzyme complex component, ATP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 372,419 | 0 | G | C | 25.2% | 155.6 / 53.3 | 99 | T1122T (ACG→ACC) | entF | enterobactin synthase multienzyme complex component, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (56/18); new base C (17/8); total (73/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01 | |||||||||||
AATGGCCCCATGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGC > minE/372360‑372480 | aaTGGCCCCATGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGa > 1:3355157/1‑68 (MQ=255) gCCCCATGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGaacaac < 1:2212775/69‑1 (MQ=255) tGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGc > 1:2720023/1‑69 (MQ=255) tGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGc > 1:376769/1‑69 (MQ=255) tGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGc > 1:3002487/1‑69 (MQ=255) gCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGaac > 1:1755027/1‑59 (MQ=255) gCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCa > 1:3005076/1‑69 (MQ=255) gCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCa > 1:2294720/1‑69 (MQ=255) gCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCa > 1:2208046/1‑69 (MQ=255) gCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCa > 1:2321132/1‑69 (MQ=255) gCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCa > 1:1977913/1‑69 (MQ=255) gCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCa > 1:2728037/1‑69 (MQ=255) gCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCa > 1:2732047/1‑69 (MQ=255) gCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCa > 1:2949730/1‑69 (MQ=255) gCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCa > 1:1724724/1‑69 (MQ=255) gCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCa > 1:3165746/1‑69 (MQ=255) gCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCa > 1:660780/1‑69 (MQ=255) gCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCa > 1:3288251/1‑69 (MQ=255) gCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCa > 1:3318361/1‑69 (MQ=255) gCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCa > 1:1274096/1‑69 (MQ=255) gCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCa > 1:1135770/1‑69 (MQ=255) gCAGACGGCGGCAAACCTGGATGAAGTCTGAGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCa > 1:2024109/1‑69 (MQ=255) cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTg > 1:2760722/1‑55 (MQ=255) cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAGCAACCGCAt > 1:1907533/1‑69 (MQ=255) cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCa > 1:1154641/1‑68 (MQ=255) cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:358798/1‑69 (MQ=255) cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:99062/1‑69 (MQ=255) cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:79649/1‑69 (MQ=255) cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:796480/1‑69 (MQ=255) cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:2481857/1‑69 (MQ=255) cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:531600/1‑69 (MQ=255) cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:3166169/1‑69 (MQ=255) cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:277768/1‑69 (MQ=255) cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:2888820/1‑69 (MQ=255) cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:3249783/1‑69 (MQ=255) cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:990802/1‑69 (MQ=255) cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:3031336/1‑69 (MQ=255) cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:3143441/1‑69 (MQ=255) cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:1475018/1‑69 (MQ=255) cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:1383404/1‑69 (MQ=255) cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:1486327/1‑69 (MQ=255) cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:2208952/1‑69 (MQ=255) aCGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGc < 1:97167/69‑1 (MQ=255) aCGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGc < 1:189660/69‑1 (MQ=255) cggcAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCtt < 1:529112/69‑1 (MQ=255) cggcAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCtt < 1:1069181/69‑1 (MQ=255) cggcAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCtt < 1:2477982/69‑1 (MQ=255) aaaCCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCttatta < 1:3423884/69‑1 (MQ=255) aaaCCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCttatta < 1:1847800/69‑1 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTgggg < 1:1972450/69‑1 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTgggg < 1:2634187/69‑1 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTgggg < 1:3349000/69‑1 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTgggg < 1:2205727/69‑1 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGaacaaca > 1:127925/1‑41 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:3113843/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:583034/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:1097823/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:3213330/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:1294558/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:3254701/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:1100941/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:1284053/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:529773/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:769735/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:2208275/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:2690055/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:840003/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:1806779/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:1532792/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:1660773/1‑65 (MQ=255) aaGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTgggg < 1:3122691/68‑1 (MQ=255) aaGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGt > 1:2118446/1‑69 (MQ=255) tGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTcc < 1:398995/69‑1 (MQ=255) aGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCGCtt < 1:2995029/44‑1 (MQ=255) tCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTAc < 1:293269/69‑1 (MQ=255) tCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTAc < 1:3048020/69‑1 (MQ=255) tCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTAc < 1:117501/69‑1 (MQ=255) tGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGc > 1:751419/1‑69 (MQ=255) tGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGc > 1:2113597/1‑69 (MQ=255) tGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGc > 1:1460471/1‑69 (MQ=255) tGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGc > 1:111651/1‑69 (MQ=255) tGGCAACCTTACTTGAACAACAACCGCACGGCCCatat < 1:850982/38‑1 (MQ=25) tGGCAACCTTACTTGAACAACAACCGCACGGCCCatat < 1:542860/38‑1 (MQ=25) tGGCAACCTTACTTGAACAACAACCGCACGGCCCatat < 1:1044338/38‑1 (MQ=25) tGGCAACCTTACTTGAACAACAACCGCACGGCCCatat < 1:343887/38‑1 (MQ=25) tGGCAACCTTACTTGAACAACAACCGCACGGCCCatat < 1:3372855/38‑1 (MQ=25) tGGCAACCTTACTTGAACAACAACCGCACGGCCCatat < 1:3099236/38‑1 (MQ=25) tGGCAACCTTACTTGAACAACAACCGCACGGCCCatat < 1:1870220/38‑1 (MQ=25) tGGCAACCTTACTTGAACAACAACCGCACGGCCCatat < 1:2673422/38‑1 (MQ=25) gCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:1260134/1‑53 (MQ=255) gCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:3370508/1‑53 (MQ=255) gCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:1292728/1‑53 (MQ=255) gCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:3175504/1‑53 (MQ=255) gCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:2904687/1‑53 (MQ=255) gCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:2790243/1‑53 (MQ=255) gCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:989472/1‑53 (MQ=255) gCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:1901464/1‑53 (MQ=255) gCAACGTTACTTGAACAAAAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:3056951/1‑53 (MQ=255) gCAACGTTAATTGAACAACAACCGCATGGCCCTTATTAc > 1:1082974/1‑39 (MQ=255) | AATGGCCCCATGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGC > minE/372360‑372480 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |