| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 372,440 | T→C | 33.3% | H1129H (CAT→CAC) | entF → | enterobactin synthase multienzyme complex component, ATP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 372,440 | 0 | T | C | 33.3% | 54.8 / 37.6 | 72 | H1129H (CAT→CAC) | entF | enterobactin synthase multienzyme complex component, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (26/22); new base C (16/8); total (42/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
CAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGCTGGCGCAGGGT > minE/372372‑372491 | cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAGCAACCGCAt > 1:1907533/1‑69 (MQ=255)cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:3166169/1‑69 (MQ=255)cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:1475018/1‑69 (MQ=255)cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:1486327/1‑69 (MQ=255)cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:2208952/1‑69 (MQ=255)cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:2481857/1‑69 (MQ=255)cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:2888820/1‑69 (MQ=255)cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:3031336/1‑69 (MQ=255)cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:3143441/1‑69 (MQ=255)cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:277768/1‑69 (MQ=255)cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:3249783/1‑69 (MQ=255)cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:358798/1‑69 (MQ=255)cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:531600/1‑69 (MQ=255)cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:796480/1‑69 (MQ=255)cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:79649/1‑69 (MQ=255)cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:99062/1‑69 (MQ=255)cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:990802/1‑69 (MQ=255)cAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt > 1:1383404/1‑69 (MQ=255) aCGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGc < 1:189660/69‑1 (MQ=255) aCGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGc < 1:97167/69‑1 (MQ=255) cggcAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCtt < 1:529112/69‑1 (MQ=255) cggcAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCtt < 1:2477982/69‑1 (MQ=255) cggcAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCtt < 1:1069181/69‑1 (MQ=255) aaaCCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCttatta < 1:1847800/69‑1 (MQ=255) aaaCCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCttatta < 1:3423884/69‑1 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTgggg < 1:2634187/69‑1 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTgggg < 1:1972450/69‑1 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTgggg < 1:2205727/69‑1 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTgggg < 1:3349000/69‑1 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:3254701/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:1100941/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:2690055/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:2208275/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:529773/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:1097823/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:3113843/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:1294558/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:583034/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:769735/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:840003/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:3213330/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:1806779/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:1660773/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:1532792/1‑65 (MQ=255) gAAGTCTGCGAAGCGCATCTGGCAACCTTACTTGAACAACAACCGCACGGCCCATATTACctgct > 1:1284053/1‑65 (MQ=255) aaGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTgggg < 1:3122691/68‑1 (MQ=255) aaGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGt > 1:2118446/1‑69 (MQ=255) tGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTcc < 1:398995/69‑1 (MQ=255) aGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCGCtt < 1:2995029/44‑1 (MQ=255) tCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTAc < 1:3048020/69‑1 (MQ=255) tCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTAc < 1:293269/69‑1 (MQ=255) tCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTAc < 1:117501/69‑1 (MQ=255) tGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGc > 1:111651/1‑69 (MQ=255) tGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGc > 1:1460471/1‑69 (MQ=255) tGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGc > 1:751419/1‑69 (MQ=255) tGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGc > 1:2113597/1‑69 (MQ=255) tGGCAACCTTACTTGAACAACAACCGCACGGCCCatat < 1:3372855/38‑1 (MQ=25) tGGCAACCTTACTTGAACAACAACCGCACGGCCCatat < 1:343887/38‑1 (MQ=25) tGGCAACCTTACTTGAACAACAACCGCACGGCCCatat < 1:542860/38‑1 (MQ=25) tGGCAACCTTACTTGAACAACAACCGCACGGCCCatat < 1:850982/38‑1 (MQ=25) tGGCAACCTTACTTGAACAACAACCGCACGGCCCatat < 1:1044338/38‑1 (MQ=25) tGGCAACCTTACTTGAACAACAACCGCACGGCCCatat < 1:1870220/38‑1 (MQ=25) tGGCAACCTTACTTGAACAACAACCGCACGGCCCatat < 1:2673422/38‑1 (MQ=25) tGGCAACCTTACTTGAACAACAACCGCACGGCCCatat < 1:3099236/38‑1 (MQ=25) gCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:3175504/1‑53 (MQ=255) gCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:989472/1‑53 (MQ=255) gCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:1901464/1‑53 (MQ=255) gCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:2790243/1‑53 (MQ=255) gCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:1292728/1‑53 (MQ=255) gCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:3370508/1‑53 (MQ=255) gCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:2904687/1‑53 (MQ=255) gCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:1260134/1‑53 (MQ=255) gCAACGTTACTTGAACAAAAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:3056951/1‑53 (MQ=255) gCAACGTTAATTGAACAACAACCGCATGGCCCTTATTAc > 1:1082974/1‑39 (MQ=255) aCTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGCTGGCGCAggg < 1:1400537/69‑1 (MQ=255) ttGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGCTGGCGCAGGGt < 1:3197962/68‑1 (MQ=255) gAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:1442405/1‑41 (MQ=255) gAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:1538793/1‑41 (MQ=255) gAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:3164740/1‑41 (MQ=255) gAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc < 1:1598536/41‑1 (MQ=255) gAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:1089326/1‑41 (MQ=255) gAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:2257210/1‑41 (MQ=255) gAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:2404802/1‑41 (MQ=255) gAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc < 1:2540721/41‑1 (MQ=255) gAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:2566572/1‑41 (MQ=255) gAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:2620021/1‑41 (MQ=255) gAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc < 1:3131442/41‑1 (MQ=255) gAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:1034948/1‑41 (MQ=255) gAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:386230/1‑41 (MQ=255) gAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTc > 1:2842887/1‑41 (MQ=255) | CAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGCTGGCGCAGGGT > minE/372372‑372491 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |