| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 372,467 | C→A | 62.0% | S1138S (TCC→TCA) | entF → | enterobactin synthase multienzyme complex component, ATP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 372,467 | 0 | C | A | 62.0% | 9.3 / 22.8 | 29 | S1138S (TCC→TCA) | entF | enterobactin synthase multienzyme complex component, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (4/7); new base A (7/11); total (11/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01 | |||||||||||
TGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGC > minE/372399‑372529 | tGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTcc < 1:398995/69‑1 (MQ=255) tCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTAc < 1:117501/69‑1 (MQ=255) tCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTAc < 1:3048020/69‑1 (MQ=255) tCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTAc < 1:293269/69‑1 (MQ=255) tGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGc > 1:1460471/1‑69 (MQ=255) tGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGc > 1:111651/1‑69 (MQ=255) tGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGc > 1:751419/1‑69 (MQ=255) tGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGc > 1:2113597/1‑69 (MQ=255) aCTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGCTGGCGCAggg < 1:1400537/69‑1 (MQ=255) ttGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGCTGGCGCAGGGt < 1:3197962/68‑1 (MQ=255) taCCTGCTGGGGTATTCCCTTGGCGGTACGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTg < 1:1652562/67‑1 (MQ=255) tgctgGGTTATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCg > 1:1352968/1‑61 (MQ=255) tgctgGGTTATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAAc > 1:524889/1‑69 (MQ=39) tgctgGGTTATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAAc > 1:1826873/1‑69 (MQ=39) tgctgGGTTATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAAc > 1:2272703/1‑69 (MQ=39) tgctgGGTTATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAAc > 1:2465860/1‑69 (MQ=39) tgctgGGTTATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAAc > 1:2579821/1‑69 (MQ=39) tgctgGGTTATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAAc > 1:3429452/1‑69 (MQ=39) gctgGGGTATTCCCTTGGCGGTACGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTg < 1:2007268/62‑1 (MQ=255) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:583376/68‑1 (MQ=39) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:3382497/68‑1 (MQ=39) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:92744/68‑1 (MQ=39) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:3223445/68‑1 (MQ=39) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:3130289/68‑1 (MQ=39) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:2970658/68‑1 (MQ=39) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:2522928/68‑1 (MQ=39) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:1915921/68‑1 (MQ=39) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:1812631/68‑1 (MQ=39) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:1796389/68‑1 (MQ=39) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:1521132/68‑1 (MQ=39) | TGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGC > minE/372399‑372529 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |