| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 372,491 | T→C | 53.0% | G1146G (GGT→GGC) | entF → | enterobactin synthase multienzyme complex component, ATP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 372,491 | 0 | T | C | 53.0% | 0.8 / 24.4 | 34 | G1146G (GGT→GGC) | entF | enterobactin synthase multienzyme complex component, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (3/13); new base C (7/11); total (10/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGCATTTCTTGGCTTGCTGGATACCTGGCC > minE/372424‑372556 | ttGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGCTGGCGCAGGGt < 1:3197962/68‑1 (MQ=255) taCCTGCTGGGGTATTCCCTTGGCGGTACGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTg < 1:1652562/67‑1 (MQ=255) tgctgGGTTATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCg > 1:1352968/1‑61 (MQ=255) tgctgGGTTATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAAc > 1:2272703/1‑69 (MQ=39) tgctgGGTTATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAAc > 1:2465860/1‑69 (MQ=39) tgctgGGTTATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAAc > 1:2579821/1‑69 (MQ=39) tgctgGGTTATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAAc > 1:1826873/1‑69 (MQ=39) tgctgGGTTATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAAc > 1:3429452/1‑69 (MQ=39) tgctgGGTTATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAAc > 1:524889/1‑69 (MQ=39) gctgGGGTATTCCCTTGGCGGTACGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTg < 1:2007268/62‑1 (MQ=255) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:3223445/68‑1 (MQ=39) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:3382497/68‑1 (MQ=39) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:3130289/68‑1 (MQ=39) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:2970658/68‑1 (MQ=39) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:2522928/68‑1 (MQ=39) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:583376/68‑1 (MQ=39) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:92744/68‑1 (MQ=39) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:1521132/68‑1 (MQ=39) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:1915921/68‑1 (MQ=39) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:1812631/68‑1 (MQ=39) ttATTCACTTGGCGGTACGCTGGCGCAGGGCATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGc < 1:1796389/68‑1 (MQ=39) tACGCTGGCGCAGGGTATTGCTGCGCGACTGCGTGCCCGTGGCGAACAGGTGGCATTTCTTGGCTTGc > 1:889250/1‑68 (MQ=255) tACGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGCATTTCTTGGCTTGc > 1:1628692/1‑68 (MQ=255) aCGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGCATTTCTTGGCTTGCTg < 1:1035685/69‑1 (MQ=255) aCGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGCATTTCTTGGCTTGCTg < 1:2247538/69‑1 (MQ=255) aCGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGCATTTCTTGGCTTGCTg < 1:843068/69‑1 (MQ=255) aCGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGCATTTCTTGGCTTGCTg < 1:1189004/69‑1 (MQ=255) aCGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGCATTTCTTGGCTTGCTg < 1:563706/69‑1 (MQ=255) aCGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGCATTTCTTGGCTTGCTg < 1:1783870/69‑1 (MQ=255) aCGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGCATTTCTTGGCTTGCTg < 1:3277477/69‑1 (MQ=255) aCGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGCATTTCTTGGCTTGCTg < 1:3168836/69‑1 (MQ=255) aCGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGCATTTCTTGGCTTGCTg < 1:2963004/69‑1 (MQ=255) aCGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGCATTTCTTGGCTTGCTg > 1:2903532/1‑69 (MQ=255) aCGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGCATTTCTTGGCTTGCTg < 1:1945761/69‑1 (MQ=255) aCGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGCATTTCTTGGCTTGCTg < 1:2066157/69‑1 (MQ=255) tATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGCATTTCTTGGCTTGCTg < 1:2786992/55‑1 (MQ=255) tATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGCATTTCTTGGCTTGCTGGATACCTGgcc > 1:1792650/1‑66 (MQ=255) tATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGCATTTCTTGGCTTGCTGGATACCTGgcc > 1:2818635/1‑66 (MQ=255) | TTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGCGGTACGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGCATTTCTTGGCTTGCTGGATACCTGGCC > minE/372424‑372556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |