| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 373,549 | G→A | 23.7% | E133E (GAG→GAA) | fepE → | regulator of length of O‑antigen component of lipopolysaccharide chains |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 373,549 | 0 | G | A | 23.7% | 144.0 / 35.8 | 110 | E133E (GAG→GAA) | fepE | regulator of length of O‑antigen component of lipopolysaccharide chains |
| Reads supporting (aligned to +/- strand): ref base G (59/24); new base A (8/18); total (67/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.04e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 125 total aligned reads displayed.
GGTTAGCTTGCTGGAAGAGTACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTT > minE/373489‑373615 | ggTTAGCTTGCTGGAAGAGTACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGaaaa > 1:2023772/1‑68 (MQ=255)ggTTAGCTTGCTGGAAGAGTACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGaaaa > 1:227277/1‑68 (MQ=255)ggTTAGCTTGCTGGAAGAGTACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGaaaa > 1:1844082/1‑68 (MQ=255)ggTTAGCTTGCTGGAAGAGTACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGaaaa > 1:3331326/1‑68 (MQ=255) ttAGCTTGCTGGAAGAGTACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATc < 1:61965/68‑1 (MQ=255) tAGCTTGCTGGAAGAGTACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATcg > 1:1795268/1‑68 (MQ=255) tAGCTTGCTGGAAGAGTACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATcg > 1:244929/1‑68 (MQ=255) tAGCTTGCTGGAAGAGTACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATcg > 1:1577565/1‑68 (MQ=255) tAGCTTGCTGGAAGAGTACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATcg > 1:3363131/1‑68 (MQ=255) tAGCTTGCTGGAAGAGTACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATc > 1:1625959/1‑67 (MQ=255) agTACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGc < 1:1027761/69‑1 (MQ=255) agTACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGc < 1:1107463/69‑1 (MQ=255) agTACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGc < 1:79417/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTTAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:2831428/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:479373/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:101392/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:1454295/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:839557/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:2769417/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:1195702/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:1592560/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:2757906/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:2412929/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:2644204/69‑1 (MQ=255) tACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCAt > 1:1843938/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATcgacga > 1:875730/1‑54 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATcgacga > 1:2429065/1‑54 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATcgacga > 1:832966/1‑54 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATcgacga < 1:2705010/54‑1 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATcgacga > 1:3074205/1‑54 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATcgacga > 1:602756/1‑54 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATcgacga > 1:546724/1‑54 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATcgacga > 1:3374615/1‑54 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATcgacga > 1:373533/1‑54 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATcgacga < 1:1245647/54‑1 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATcgacga > 1:1231593/1‑54 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATcgacga > 1:1618448/1‑54 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATcgacca < 1:643057/54‑3 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCAt > 1:2049714/1‑67 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:2179367/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:2468898/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:2426194/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:2300291/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:2277955/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:2662145/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:347173/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:2050860/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:1876394/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:1500968/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:3262185/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATc > 1:1779859/1‑68 (MQ=255) tGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcgc > 1:48746/1‑68 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc > 1:1544905/1‑69 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc > 1:1127555/1‑69 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc > 1:313586/1‑69 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc < 1:1349102/69‑1 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc > 1:288359/1‑69 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc > 1:2876191/1‑69 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc < 1:3387097/69‑1 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc < 1:44285/69‑1 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc < 1:1718465/69‑1 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc > 1:2157912/1‑69 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc > 1:1687716/1‑69 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc > 1:2685707/1‑69 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc > 1:484610/1‑69 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc < 1:2588065/69‑1 (MQ=255) ttCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCa < 1:2732224/67‑1 (MQ=255) ttCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCa < 1:3123218/67‑1 (MQ=255) ttCATCACCTTATGCGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAAt < 1:186153/69‑1 (MQ=255) catcaCCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCATCGCGCCATTg > 1:653184/1‑69 (MQ=255) catcaCCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCATCGCGCCATTg > 1:472056/1‑69 (MQ=255) catcaCCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCATCGCGCCATTg > 1:1127733/1‑69 (MQ=255) catcaCCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCATCGCGCCATTg > 1:1923087/1‑69 (MQ=255) catcaCCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCATCGCGCCATTg > 1:3104746/1‑69 (MQ=255) catcaCCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCATCGCGCCATTg > 1:2840410/1‑69 (MQ=255) caCCTTATGTGATGGATCAATTAAAAGAAGCGATAATCGACCCACTGGATCTGCa < 1:2664771/55‑1 (MQ=255) caCCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCTCAATTGTCg < 1:373303/69‑1 (MQ=255) caCCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCg < 1:1914623/69‑1 (MQ=255) caCCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCg < 1:1744742/69‑1 (MQ=255) tATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTg > 1:1416194/1‑69 (MQ=255) tATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTg > 1:1053526/1‑69 (MQ=255) tATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTg > 1:2145637/1‑69 (MQ=255) tATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTg > 1:1297706/1‑69 (MQ=255) tATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTg > 1:1508458/1‑69 (MQ=255) tgtgATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCg < 1:784554/62‑1 (MQ=255) aTGGATCAATTAAAAGACGCGAAAATCGACCCACTGGATCTGCa < 1:858057/44‑1 (MQ=21) aaaaGAGGCGAAAATCGACGAACTGGATTTGCATCGCGTAATTGTCGCATTGAGCGAAAAAATGAAAGc > 1:1330454/1‑69 (MQ=255) aaaaGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGc > 1:3382077/1‑69 (MQ=255) gagTCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:1230073/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCTAAAAAATGAAAGCGGtt < 1:3257639/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:2294863/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:2161973/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:1414444/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:659153/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:254274/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:117502/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:1978313/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:3332300/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:1704676/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:280324/69‑1 (MQ=255) | GGTTAGCTTGCTGGAAGAGTACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTT > minE/373489‑373615 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |